PHYTOCHEMICAL ANALYSIS AND EVALUATION OF ANTIMICROBIAL ACTIVITY IN THE WHOLE PLANT EXTRACTS OF GLORIOSA SUPERBA
Keywords:Flame lily,, Alkaloids,, Phenolic compounds,, Antibacterial,, Antifungal activity
Objectives: The aim of this research work was to carry out the phytochemical screening and to evaluate the antibacterial and antifungal activity of the whole plant extract (shoot, flower, and tuber) of the flame lily (Gloriosa superba).
Materials and Methods: In the current work, phytochemicals were extracted from different parts of the plant using different solvents dimethyl sulfoxide, ethyl acetate, and ethanol (ETOH). These phytochemicals contained alkaloids, flavonoids, terpenoids, tannins, saponins, aromatic acids, phenolic compounds, xanthoproteins, triterpenoids, amino acids, philobatinins, carbohydrate, reducing sugar, and proteins and they were separated using standard methods. Furthermore, antimicrobial activities of methanolic separation were determined using various species of bacteria and fungi. Agar well diffusion method was used for the antimicrobial activity and the zone of the inhibition was analyzed.
Results: The evaluation of preliminary phytochemical screening of extracts indicated the presence of carbohydrates, reducing sugars, proteins, amino acids, steroids, flavonoids, terpenoids, saponins, alkaloids, tannins, and phlobatannins. Tuber extracts highlighted effective antibacterial and antifungal activities compared with shoot and flower extracts against all the tested bacteria and fungi. ETOH extract of the tuber observed highest antibacterial activity against Staphylococcus aureus (19 mm), followed by Escherichia coli (18 mm), Micrococcus luteus (17 mm), Pseudomonas aeruginosa (17 mm), and Salmonella abony (16 mm) when compared to shoot and flower extracts. G. superba tuber extracts highlighted effective antifungal activities compared with shoot and flower extracts against all the tested fungi. ETOH extract of the tuber observed highest antifungal activity against Rhizopus oryzae (20.17 mm), followed by Mucor Sp. (19.87 mm), Aspergillus niger (18.02), Candida krusei (17.98 mm), and Candida albicans (16.88 mm) as compared to shoot and flower extracts.
Conclusion: Results of the current studies flame lily ETOH extracts showed that the plant has significant antimicrobial activities. The strong antibacterial and antifungal activities of flame lily are due to the presence of tannins and flavonoids present in.
Ballabh B, Chaurasia OP. Traditional medicinal plants of cold desert ladakh-used in treatment of cold, cough and fever. J Ethnopharmacol 2007;112:341-9.
Perumal Samy R, Ignacimuthu S, Sen A. Screening of 34 Indian medicinal plants for antibacterial properties. J Ethnopharmacol 1998;62:173-82.
Leena KP, Sreelakshmi KS. Phytochemical screening and in vitro cytotoxicity studies of Mussaenda frondosa Linn leaves. Res J Pharm Technol 2017;10:4227-30.
Sreesha NN, Alexeyena V, Meenu B, Greeshma R, Neeraja ED, et al. Comparative evaluation of Coriandrum sativum Linn. And Apius graveolens for antimicrobial activity. Res J Pharm Technol 2017;10:541-4.
Shaikh S, Jain V. Development and validation of RP-HPLC method for the simultaneous determination of curcumin, piperine and camphor in an ayurvedic formulation. Int J Pharm Pharm Sci 2018;10:115-21.
Maroyi A, Van der Maesen LJ. Gloriosa superba L. (family colchicaceae): Remedy or poison? Med Plants Res 2011;5:6112-21.
Trease SE, Evans D. Colchicum seed and corn. In: Pharmacognosy. 12th ed. London: Balliere Tindall; 1983. p. 593-7.
Ade R, Rai MK. Review: Current advances in Gloriosa superba L. Biodiversitas 2009;10:210-4.
Alagesaboopathi C. Antimicrobial screening of selected medicinal plants in Tamilnadu, India. Afr J Microbiol Res 2011;5:617-21.
Hemaiswarya S, Raja R, Anbazhagan C, Thiagarajan V. Antimicrobial and mutagenic properties of the root tubers of Gloriosa superba Linn. Pak J Bot 2009;41:293-9.
Srivastava UC, Chandra V. Gloriosa superba Linn. (Kalihari)-an important colchicine producing plant. J Res Indian Med 1977;10:92-5.
Edeoga HO, Okwu DE, Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. Afr J Biotechnol 2005;4:685-8.
Kala CP. Indigenous uses and sustainable harvesting of trees by local people in the pachmarhi biosphere reserve of India. Int J Med Aromat Plants 2011;1:153-61.
Evans WC. Trees and Evan Pharmacology. 14th ed. Philadelphia, PA: W.B. Saunders Co. Ltd., 2000. p. 224-93.
Ghani A. Medicinal Plants of Bangladesh with Chemical Constituents and Uses. 2nd ed. Dhaka, Bangladesh: Asiatic Society of Bangaladesh; 2003. p. 603.
Harbone JB. Phytochemical Methods-a Guide to Modern Technique of Plant Analysis. London: Chapmen and Hall; 1998. p. 182-90.
Aberkane A, Cuenca-Estrella M, Gomez-Lopez A, Petrikkou E, Mellado E, Monzón A, et al. Comparative evaluation of two different methods of inoculum preparation for antifungal susceptibility testing of filamentous fungi. J Antimicrob Chem 2002;50:719-22.
Eloff JN. Which extractant should be used for the screening and isolation of antimicrobial components from plants? J Ethnopharmacol 1998;60:1-8.
Eloff JN, Picard J, Masoko P. Resistance of animal fungal pathogens to solvents used in bioassays. S Afr J Bot 2007;73:667-9.
Prakash S, Jain AK. Antifungal activity and preliminary phytochemical studies of leaf extract of Solanum nigrumlinn. Int J Pharm Pharm Sci 2011;3:352-5.
Kalita L, Dash B, Borah U, Deka J, Dash S. Preliminary phytochemical analysis and antimicrobial activity ethanolic extracts of dried fruits of Solanum torvum (Family-Solanaceae). Int J Curr Pharm Res 2017;9:123-6.
Sivakumar T. Phytochemical screening and gas chromatography-mass spectroscopy analysis of bioactive compounds and biosynthesis of silver nanoparticles using sprout extracts of Vigna radiata L. And their antioxidant and antibacterial activity. Asian J Pharm Clin Res 2019;12:180-4.
Sivakumar T, Gajalakshmi D. Phytochemical screening and gc-ms analysis of root extract from Asparagus racemosus L. Int J Pharm Sci Res 2014;5:5245-9.
Sivakumar T, Gajalakshmi D. In vitro antioxidant and chemical constituents from the leaves of Ormocarpum cochinchinense Elumbotti. Am J Plant Physiol 2013;8:114-22.
Sivakumar T, Gajalakshmi D. Gas chromatography-mass spectroscopy analysis of Ormocarpum cochinchinense leaf extract-traditional bone healing plants. Int J Pharm Res Bio Sci 2014;3:352-9.
Sivakumar T, Panneerselvam R. Triadiadomefon mediated changes in antioxidant and Indole alkaloid content in two species of Datura. Am J Plant Physiol 2011;6:252-60.
Senthilkumar SR, Sivakumar T. Green tea (Camellia sinensis) mediated synthesis of zinc oxide (ZNO) nanoparticles and studies on their antimicrobial activities. Int J Pharm Pharm Sci 2014;6:461-5.
Bhavani D, Malairajan S, Vanitha V. Physicochemical, phytochemicals and antioxidant evaluation of Guazuma ulmifolia fruit 2018. Int J Pharm Pharm Sci 2018;10:87-91.
Kuete V, Nguemeving JR, Beng VP, Azebaze AG, Etoa FX, Meyer M. Antimicrobial activity of the methanol extracts and compounds from Vismia laurentii De Wild (Guttiferae). J Ethnopharmacol 2007;109:372-9.
Ikigai H, Nakae T, Hara Y, Shimamura T. Bactericidal catechins damage the lipid bilayer. Biochem Biophys Acta 1993;1147:132-6.
Jigna PA, Darshana JA, Sumitra CH. Efficacy of aqueous and methanol extracts of some medicinal plants for potential antibacterial activity. Turk J Biol 2005;29:203-10.
Sivakumar T, Gajalakshmi D, Subramanian VK, Palanisamy K. Tuber extract mediated biosynthesis of silver nanoparticles and its antioxidant, antibacterial activity. J Biol Sci 2015;15:68-7.
Nagarasan S, Boominathan M. Perspective pharmacological activities of Leucas aspera: An indigenous plant species. Indo Am J Pharm Res 2016;6:6567-72.
Akter M, Khan MA, Muhsin MD, Hamid K, Ullah MO, Bulbul IJ. In vitro studies on antibacterial, antifungal, and cytotoxic properties of Leucas aspera. Biol Med 2012;4:183-7.
Tschesche R. Advances in the chemistry of antibiotics substances from higher plants. Pharmacogn Phytochem 1970;3:274-89.
Netala VR, Ghosh SB, Bobbu PL, Dandu A, Tartte V. Triterpenoid saponins: A review on biosynthesis, applications and mechanism of their action. Int J Pharm Pharm Sci 2015;7:24-8.
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