PHYSICAL AND CHEMICAL CHARACTERIZATION OF GREEN SYNTHESIZED SILVER NANOPARTICLES USING STEM OF HIBISCUS VITIFOLIUS L. AND ITS ANTIMICROBIAL AND ANTIOXIDANT POTENTIAL

  • ANTONY LAWRENCE A Department of Physics, Government Arts College (Affiliated to Bharathidasan University), Tiruchirappalli, Tamil Nadu, India.
  • THOMAS JOSEPH PRAKASH J Department of Physics, Government Arts College (Affiliated to Bharathidasan University), Tiruchirappalli, Tamil Nadu, India.

Abstract

Objectives: The aim of our work was to synthesize the silver nanoparticle (AgNP) using Hibiscus vitifolius L. stem extract its characterization and evaluation of antimicrobial and antioxidant assay.


Materials and Methods: The silver nitrate (1 mM) mixed with aqueous stem extract of H. vitifolius L. after the nanoparticles is examined by Fourier-transform infrared (FT-IR), ultraviolet-visible (UV-vis) spectroscopy, field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray (EDAX), X-ray powder diffraction (XRD), dynamic light scattering (DLS), zeta potential, thermogravimetry/differential thermal analysis (TG/DTA), and differential scanning calorimetry (DSC). The aqueous stem extract is examined for phytochemical screening, gas chromatography–mass spectrometry (GC–MS) analysis, FT-IR, and UV-vis spectroscopy. The antibacterial, antifungal, and antioxidant assay were also evaluated for the AgNPs.


Results: The aqueous stem extract shows 20 compounds in GC–MS analysis. The FT-IR and UV-vis spectroscopy show the biocompounds. H. vitifolius stem extract-AgNPs (HVS-AgNPs) examined in UV and FT-IR shows the presence of AgNPs, FE-SEM shows that the particle size is 30–70 nm, EDAX shows the presence of silver metal, and XRD shows that the particles are face-centered cubic. DLS shows the hydrodynamic size 136.9 nm, zeta potential shows the stability (−18.6 mV), and TG/DTA and DSC show that the particles are stable up to 335°C. The HVS-AgNPs are also evaluated in antimicrobial and antioxidant potential and the report shows a good inhibition.


Conclusion: The stem extract of H. vitifolius L. can be used for green synthesis of AgNPs and could be used as antimicrobial and antioxidant potential.

Keywords: Antimicrobial activity,, Antioxidant assay,, Characterization,, Hibiscus vitifolius L.,, Silver nanoparticles.

Author Biographies

ANTONY LAWRENCE A, Department of Physics, Government Arts College (Affiliated to Bharathidasan University), Tiruchirappalli, Tamil Nadu, India.

PG & Research Department of Physics, Government Arts College (Affiliated to Bharathidasan University), Trichy-620022.

THOMAS JOSEPH PRAKASH J, Department of Physics, Government Arts College (Affiliated to Bharathidasan University), Tiruchirappalli, Tamil Nadu, India.

PG& Research Department of Physics,Government Arts College,Trichy-22.

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ANTONY LAWRENCE A, and THOMAS JOSEPH PRAKASH J. “PHYSICAL AND CHEMICAL CHARACTERIZATION OF GREEN SYNTHESIZED SILVER NANOPARTICLES USING STEM OF HIBISCUS VITIFOLIUS L. AND ITS ANTIMICROBIAL AND ANTIOXIDANT POTENTIAL”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 6, Apr. 2019, pp. 46-53, https://innovareacademics.in/journals/index.php/ajpcr/article/view/33113.
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