INHIBITION ACTIVITY OF WATER HYACINTH LEAF EXTRACT (EICHHORNIA CRASSIPES) AGAINST AGGREGATIBACTER ACTINOMYCETEMCOMITANS

  • YASINTA IZZAH AFIDATI Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
  • IRMA JOSEFINA SAVITRI Department of Periodontology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
  • AGUNG KRISMARIONO Department of Periodontology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.

Abstract

Objective: Water hyacinth (Eichhornia crassipes) contains bioactive compounds that have antibacterial properties. The antibacterial agent is required in the treatment of periodontitis. The aim of this study was to determine the inhibition activity of water hyacinth leaf extract against Aggregatibacter actinomycetemcomitans that was the major cause of aggressive periodontitis.


Methods: This research divided into two groups (treatment and control group). The treatment group was conducted using a serial dilution of water hyacinth leaf extract at concentrations of 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56%, and 0.78% in brain heart infusion broth media with A. actinomycetemcomitans suspension. The control group was prepared without water hyacinth leaf extract. All media were incubated at 37°C for 24 h. Inhibition activity was measured by calculates the number of bacterial colonies on Mueller-Hinton agar media. The data were statistical analysis using one-way ANOVA.


Results: There were significant differences between the treatment group (concentrations of 100% to 1.56%) and the control group (p < 0.05). Water hyacinth leaf extract at concentrations of 100% to 6.25% showed that there was no growth of A. actinomycetemcomitans.


Conclusion: The results suggest that water hyacinth leaf extract has inhibition activity against A. actinomycetemcomitans at a minimal concentration of 1.56%.

Keywords: Water hyacinth,, antibacterial,, Aggregatibacter actinomycetemcomitans.

References

1. Vargas SA, Ilyina A, Segura CE, Silva BY, Mendez GL. Etiology and microbiology of periodontal diseases: A review. Afr J Microbiol Res 2015;9:2300-6.
2. Tonetti MS, Jepsen S, Jin L, Otomo-Corgel J. Impact of the global burden of periodontal diseases on health, nutrition and wellbeing of mankind: A call for global action. J Clin Periodontol 2017;44:456-62.
3. Fine DH, Patil AG, Loos BG. Classification and diagnosis of aggressive periodontitis. J Periodontol 2018;89 Suppl 1:S103-19.
4. Johansson A. Aggregatibacter actinomycetemcomitans leukotoxin: A powerful tool with capacity to cause imbalance in the host inflammatory response. Toxins (Basel) 2011;3:242-59.
5. Lakhdar L, Farah A, Lahlou I, Rida S, Bouziane A, Ennibi O. Antibacterial effectiveness of selected Moroccan essential oils against the highly virulent jp2 clone of Aggregatibacter actinomycetemcomitans. Int J Pharm Pharm Sci 2017;9:47-51.
6. Tulika T, Mala A. Pharmaceutical potential of aquatic plant Pistia stratiotes (L.) and Eichhornia crassipes. J Plant Sci 2015;3:10-8.
7. Vanathi P, Rajiv P, Sivaraj R. Eichhornia mediated copper oxide nanoparticles: In vitro analysis of antimicrobial activity. Int J Pharm Pharm Sci 2015;7:422-4.
8. Haggag MW, Abou el Ella, SM, Abouziena HF. Phytochemical analysis, antifungal, antimicrobial activities and application of Eichhornia crassipes against some plant pathogens. Planta Daninha 2017;35:1-11.
9. Lakhdar L, Farah A, Bajjou T, Rida S, Bouziane A, Ennibi O. In vitro antibacterial activity of essentials oils from Mentha pulegium, Citrus aurantium and Cymbopogon citratus on virulent strains of Aggregatibacter actinomycetemcomitans. Int J Pharmacogn Phytochem Res 2014;6:1035-42.
10. Rampelotto PH. Resistance of microorganisms to extreme environmental conditions and its contribution to astrobiology. Sustainability 2010;2:1602-23.
11. Sriraman P, Mohanraj R, Neelakantan P. Aggregatibacter actinomycetemcomitans in periodontal disease. Res J Pharm Biol Chem Sci 2014;5:406-19.
12. Silhavy TJ, Kahne, Walker S. The bacterial cell envelope. Cold Spring Harb Perspect Biol 2010;2:1-16.
13. Thamaraiselvi, Lalitha P, Jayanthi P. Preliminary studies on phytochemicals and antimicrobial activity of solvent extracts of Eichhornia crassipes (Mart) Solms. Asian J Plant Sci Res 2012;2:115 22.
14. Lalitha P, Sripathi SK, Jayanthi P. Secondary metabolites of Eichhornia crassipes (Waterhyacinth): A review (1949 to 2011). NatProd Commun 2012;7:1249-56.
15. Sabbineni J. Phenol an effective antibacterial agent. J Med Org Chem 2016;3:182-91.
16. Liu B, Poolman B, Boersma AJ. Ionic strength sensing in living cells. ACS Chem Biol 2017;12:2510-14.
17. Micha?owicz J, Duda W. Phenols sources and toxicity. Polish J Environ Stud 2007;16:347-62.
18. Nazzaro F, Fratianni F, De Martino L, Coppola R, De Feo V. Effect of essential oils on pathogenic bacteria. Pharmaceuticals 2013;6:1451-74.
19. Jasmine R, Selvakumar BN, Daisy P. Investigating the mechanism of action of terpenoids and the effect of interfering substances on an Indian medicinal plant extract demonstrating antibacterial activity. Int J Pharm Stud Res 2011;2:19-24.
20. Cushnie TP, Lamb AJ. Antimicrobial activity of flavonoids. Int J Antimicrob Agents 2005;26:343-56.
21. Xie Y, Yang W, Tang F, Chen X, Ren L. Antibacterial activities of flavonoids: Structure-activity relationship and mechanism. Curr Med Chem 2015;22:132-49.
22. Cushnie TP, Cushnie B, Lamb AJ. Alkaloids: An overview of their antibacterial, antibiotic-enhancing and antivirulence activities. Int J Antimicrob Agents 2014;44:377-86.
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YASINTA IZZAH AFIDATI, IRMA JOSEFINA SAVITRI, and AGUNG KRISMARIONO. “INHIBITION ACTIVITY OF WATER HYACINTH LEAF EXTRACT (EICHHORNIA CRASSIPES) AGAINST AGGREGATIBACTER ACTINOMYCETEMCOMITANS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 6, Apr. 2019, pp. 122-5, https://innovareacademics.in/journals/index.php/ajpcr/article/view/33162.
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