FORMULATION DEVELOPMENT AND IN VITRO EVALUATION OF CURCUMIN-LOADED SOLID SELF-NANOEMULSIFYING DRUG DELIVERY SYSTEM FOR COLON CARCINOMA
Objective: Cancer is the deadliest disease affecting the life of the people all around the world. Colon cancer is the cancer which is affecting the colon region it is the last part of the gastrointestinal tract which is mainly responsible for the absorption of water and minerals from the food debris. Colon cancer is the second most cancer creating death in the world. It affects both male and female equally. Curcumin is a flavonoid used from decades for the treatment of various ailments including cancer. This present work is to formulate Self-nanoemulsifying drug delivery (SNEDDS) system with the help of curcumin for colon delivery.
Materials and Methods: Nanoemulsion was prepared using the curcumin pre-concentrated self-nanoemulsifying drug delivery system, with which tablets were prepared and coated with pectin followed by the evaluation test such as in vitro dissolution and cell line studies.
Results: Solubility profile of curcumin was found with a greater impact using Capmul MCM and Labrafac PG which is then added with the surfactants and co-surfactants and were converted into Nano-droplets. F1 formulation was selected after carrying out the characterisation studies and converted into a tablet dosage form and then coated with pectin, in vitro studies depicted a release of 80% in pH 6.8.
Conclusions: Formulation of a solid self-Nano emulsifying drug delivery system using curcumin was successfully carried out. From the results obtained, the formulation (F1) was selected for the formation of the tablets and the further experimental part is carried out. The tablet dosage form is then coated with pectin and used for targeting the colon cancer cells for its treatment.
2. Sinha VR, Kumria R. Polysaccharides in colon-specific drug delivery. Int J Pharm 2001;224:19-38.
3. Madhu CS, Saradha AC. Evaluation of heamagglutination and anti cancer potential from Indian dietary plants. Int J Pharm Pharm Sci 2018;10:105-8.
4. Shelat P, Mandowara VK, Gupta DG, Patel S. Formulation of curcuminoid loaded solid lipid nanoparticles in order to improve oral bioavailability. Int J Pharm Pharm Sci 2015;7:278-82.
5. Abbaspour MR, Sadeghi F, Garekani HA. Preparation and characterization of ibuprofen pellets based on eudragit RS PO and RL PO or their combination. Int J Pharm 2005;303:88-94.
6. Feng SS. Nanoparticles of biodegradable polymers for new-concept chemotherapy. Expert Rev Med Devices 2004;1:115-25.
7. Gupta AK, Pretlow TP, Schoen RE. Aberrant crypt foci: What we know and what we need to know. Clin Gastroenterol Hepatol 2007;5:526-33.
8. Abdalla A, Mäder K. Preparation and characterization of a self-emulsifying pellet formulation. Eur J Pharm Biopharm 2007;66:220-6.
9. Sahu AR, Bothara SB. Formulation and evaluation of self-microemulsifying drug delivery system of curcumin for enhanced solubility and dissolution. Asian J Pharm Clin Res 2015;29:893-913.
10. Gursoy RN, Benita S. Self-emulsifying drug delivery systems (SEDDS) for improved oral delivery of lipophilic drugs. Biomed Pharmacother 2004;58:173-82.
11. Basalious EB, Shawky N, Badr-Eldin SM. SNEDDS containing bioenhancers for improvement of dissolution and oral absorption of lacidipine. I: Development and optimization. Int J Pharm 2010;391:203 11.
12. Rakkappan C, Anbalagan S. Ultrasonic and FT-IR studies on aqueous biodegradable polymer blend solution. Am Eurasian J Agric Environ Sci 2009;4:281-4.
13. Chukwumezie BN, Wojcik M, Malak P, Adeyeye MC. Feasibility studies in spheronization and scale-up of ibuprofen microparticulates using the rotor disk fluid-bed technology. AAPS PharmSciTech 2002;3:E2.
14. Liew CV, Chua SM, Heng PW. Elucidation of spheroid formation with and without the extrusion step. AAPS PharmSciTech 2007;8:E70-81.
15. Cosijns A, Nizet D, Nikolakakis I, Vervaet C, De Beer T, Siepmann F, et al. Porous pellets as drug delivery system. Drug Dev Ind Pharm 2009;35:655-62.
16. Narkhede RS, Gujar KN, Gambhire VM. Design and evaluation of self-nanoemulsifying drug delivery systems for nebivolol hydrochloride. Asian J Pharm 2014;53:65-73.
17. Rowe RC, York P, Colbourn EA, Roskilly SJ. The influence of pellet shape, size and distribution on capsule filling a preliminary evaluation of three-dimensional computer simulation using a monte-carlo technique. Int J Pharm 2005;300:32-7.
18. Shaji J, Joshi V. Self-microemulsifying drug delivery system (SMEDDS) for improvingbioavailability of hydrophobic drugs and its potential to give sustained release dosageforms. Indian J Pharm Educ 2005;39:130-5.
19. Gohel MC, Parikh RK, Amin AF, Surati AK. Preparation and formulation optimization of sugar crosslinking gelatin microspheres of diclofenac sodium. Indian J Pharm Sci 2005;67:575-81.
20. Iosio T, Voinovich D, Grassi M, Pinto JF, Perissutti B, Zacchigna M, et al. Bi-layered self-emulsifying pellets prepared by co-extrusion and spheronization: Influence of formulation variables and preliminary study on the in vivo absorption. Eur J Pharm Biopharm 2008;69:686 97.
21. Kramar A, Turk S, Vrecer F. Statistical optimisation of diclofenac sustained release pellets coated with polymethacrylic films. Int J Pharm 2003;256:43-52.
22. Devi SK, Thiruganesh R, Suresh S. Preparation and characterization of pectin pellets of aceclofenac for colon targeted drug delivery. J Chem Pharm Res 2010;2:361-74.
23. Shishodia S, Sethi G, Aggarwal BB. Curcumin: Getting back to the roots. Ann N Y Acad Sci 2005;1056:206-17.
24. Sousa JJ, Sousa A, Podczeck F, Newton JM. Factors influencing the physical characteristics of pellets obtained by extrusion-spheronization. Int J Pharm 2002;232:91-106.
25. Turkoglu M, Ugurlu T. In vitro evaluation of pectin-HPMC compression coated 5-aminosalicylic acid tablets for colonic delivery. Eur J Pharm Biopharm 2002;53:65-73.
26. Liu A, Lou H, Zhao L, Fan P. Validated LC/MS/MS assay for curcumin and tetrahydrocurcumin in rat plasma and application to pharmacokinetic study of phospholipid complex of curcumin. J Pharm Biomed Anal 2006;40:720-7.
27. Gou M, Men K, Shi H, Xiang M, Zhang J, Song J, et al. Curcumin-loaded biodegradable polymeric micelles for colon cancer therapy in vitro and in vivo. Nanoscale 2011;3:1558-67.
28. Mohandas KM. Colorectal cancer in India: Controversies, enigmas and primary prevention. Indian J Gastroenterol 2011;30:3-6.
29. Kumar VK, Vennila S, Nalini N. Inhibitory effect of morin on DMH-induced biochemical changes and aberrant crypt foci formation in experimental colon carcinogenesis. Environ Toxicol Pharmacol 2010;29:50-7.
30. Pramod SN, Vigneshwaran V, Venkatesh YP. Immuno-modulatory effects of haeagglutinating lectins from potato (Solanum tuberosum) and garlic (Allium sativum) on human and murine lymphocytes. Int J Pharm Pharm Sci 2015;7:147-53.
31. Das S, Das MK. Synthesis and characterisation of thiolated jackfruit seed starch as a colonic drug delivery carrier. Int J Appl Pharm 2019;11:53-62.
32. Shaji J, Memon I. Recent advances in nanocarrier based therapeutical and diagnostic tools for colorectal cancer. Int J Curr Pharm Res 2015;7:9-16.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.