IN VITRO ASSESSMENT OF PHYSIOLOGICAL PROPERTIES OF ENTEROCOCCUS STRAINS OF HUMAN ORIGIN FOR POSSIBLE PROBIOTIC USE

Probiotic bacteria of human origin

  • ASHLESHA BHAGWAT Department of Food Engineering and Technology, Institute of Chemical Technology, Mumbai, Maharashtra, India.
  • NANDANWAR Y S Department of Obstetrics and Gynecology, Lokmanya Tilak Municipal Corporation General Hospital, Sion, Mumbai, Maharashtra, India.
  • RAHUL WARKE HiMedia Laboratories Pvt. Ltd., Mumbai, Maharashtra, India.
  • UDAY S ANNAPURE Department of Food Engineering and Technology, Institute of Chemical Technology, Mumbai, Maharashtra, India.

Abstract

Objective: The human vagina and gut are known to harbor a wide range of bacteria. Some useful bacteria maintain the vaginal pH around 3–4.5. This acidity of the vagina helps to inhibit pathogenic organisms that cause urogenital infections. The present study involved screening of previously identified Enterococcus strains of human origin for their probiotic physiological properties.


Materials and Methods: The strains were screened for their biofilm, antibiofilm, antagonistic, antibiotic resistance, adherence, aggregating, and hydrogen peroxide production abilities.


Results: Enterococcus canintestini S26B, Entamoeba dispar S27A, E. dispar S26A, E. dispar S20B, E. canintestini AB2, Enterococcus villorum SB2, and Enterococcus rivorum S22C displayed in vitro probiotic properties.


Conclusions: These strains can be used as probiotic candidates and may prove their potential in human or animal feed only after further clinical studies.

Keywords: Enterococcus,, Probiotic,, Biofilm,, Adherence,, Antimicrobial, Aggregation,, Hydrogen peroxide.

References

1. FAO and WHO. Health and Nutritional Properties of Probiotics in Food Including Powder Milk with Live Lactic Acid Bacteria. Córdoba: FAO WHO; 2001. p. 1-34.
2. Simpson KW, Rishniw M, Bellosa M, Liotta J, Lucio A, Baumgart M, et al. Influence of Enterococcus faecium SF68 probiotic on giardiasis in dogs. J Vet Intern Med 2009;23:476-81.
3. Pieniz S, Andreazza R, Anghinoni T, Camargo F, Brandelli A. Probiotic potential, antimicrobial and antioxidant activities of Enterococcus durans strain LAB18s. Food Control 2014;37:251-6.
4. Dos Santos KM, Vieira AD, Salles HO, Oliveira Jda S, Rocha CR, Borges Mde F, et al. Safety, beneficial and technological properties of Enterococcus faecium isolated from brazilian cheeses. Braz J Microbiol 2015;46:237-49.
5. Ming Lei, Xianjun Dai ML. Biological Characteristics and Safety Examination of Five Enterococcal strains from Probiotic Products : Five Enterococcal strains of five Enterococcal strains from probiotic products. J Food Safety 2016;35:324-335.
6. Wunderlich PF, Braun L, Fumagalli I, D’Apuzzo V, Heim F, Karly M, et al. Double-blind report on the efficacy of lactic acid-producing enterococcus SF68 in the prevention of antibiotic-associated diarrhoea and in the treatment of acute diarrhoea. J Int Med Res 1989;17:333-8.
7. Luong ML, Sareyyupoglu B, Nguyen MH, Silveira FP, Shields RK, Potoski BA, et al. Lactobacillus probiotic use in cardiothoracic transplant recipients: A link to invasive lactobacillus infection? Transpl Infect Dis 2010;12:561-4.
8. Bhardwaj S, Bhamre K, Dhawale J, Patil M, Divase S. Enterococus faecium and Enterococus faecalis, the nosocomial pathogens with special reference to multi-drug resistance and phenotypic characterization. Int J Pharm Sci Pract 2013;2:1-10.
9. EFSA. Guidance on the assessment of bacterial susceptibility to antimicrobials of human and veterinary importance prepared by the EFSA panel on additives and products of substances used in animal feed (FEEdAP). EFSA J 2012;10:2740.
10. Pettker CM, Buhimschi IA, Magloire LK, Sfakianaki AK, Hamar BD, Buhimschi CS, et al. Value of placental microbial evaluation in diagnosing intra-amniotic infection. Obstet Gynecol 2007;109:739-49.
11. Jiménez E, Fernández L, Marín ML, Martín R, Odriozola JM, Nueno- Palop C, et al. Isolation of commensal bacteria from umbilical cord blood of healthy neonates born by cesarean section. Curr Microbiol 2005;51:270-4.
12. Satokari R, Grönroos T, Laitinen K, Salminen S, Isolauri E. Bifidobacterium and Lactobacillus DNA in the human placenta. Lett Appl Microbiol 2009;48:8-12.
13. Pandey PK, Verma P, Kumar H, Bavdekar A, Patole MS, Shouche YS, et al. Comparative analysis of fecal microflora of healthy full-term Indian infants born with different methods of delivery (vaginal vs cesarean): Acinetobacter sp. Prevalence in vaginally born infants. J Biosci 2012;37:989-98.
14. Collins JK, Thornton G, Sullivan GO. Selection of probiotic strains for human applications. Int Dairy J 1998;8:487-90.
15. Bhardwaj A, Gupta H, Kapila S, Kaur G, Vij S, Malik RK. Safety assessment and evaluation of probiotic potential of bacteriocinogenic Enterococcus faecium KH 24 strain under in vitro and in vivo conditions. Int J Food Microbiol 2010;141:156-64.
16. Shukla G, Sharma G, Goyal N. Probiotic characterization of Lactobacilli and yeast strains isolated from whey beverage and therapeutic potential of Lactobacillus Yoghurt Murine Giardiasis 2010;2:248-61.
17. Sreeja S, Babu P R S, Prathab AG. The prevalence and the characterization of the Enterococcus species from various clinical samples in a tertiary care hospital. J Clin Diagn Res 2012;6:1486-8.
18. EFSA FEEDAP Panel. Scientific Opinion on the safety and efficacy of Enterococcus faecium (NCIMB 10415, DSM 22502, ATCC 53519 and ATCC 55593) as silage additives for all animal species 1. EFSA J 2013;11:1-22.
19. Eaton TJ, Gasson MJ. Molecular screening of Enterococcus virulence determinants and potential for genetic exchange between food and medical isolates. Appl Environ Microbiol 2001;67:1628-35.
20. Lebeer S, Verhoeven TL, Perea Vélez M, Vanderleyden J, De Keersmaecker SC. Impact of environmental and genetic factors on biofilm formation by the probiotic strain Lactobacillus rhamnosus GG. Appl Environ Microbiol 2007;73:6768-75.
21. Khiralla GM, Mohamed EA, Farag AG, Elhariry H. Antibiofilm effect of Lactobacillus pentosus and Lactobacillus plantarum cell-free supernatants against some bacterial pathogens. J Bio Res 2015;6:86-95.
22. Mahdhi A, Hmila Z, Behi A, Bakhrouf A. Preliminary characterization of the probiotic properties of candida famata and geobacillus thermoleovorans. Iran J Microbiol 2011;3:129-34.
23. Heikens E, Bonten MJ, Willems RJ. Enterococcal surface protein esp is important for biofilm formation of Enterococcus faecium E1162. J Bacteriol 2007;189:8233-40.
24. Valeriano VD, Parungao-Balolong MM, Kang DK. In vitro evaluation of the mucin-adhesion ability and probiotic potential of Lactobacillus mucosae LM1. J Appl Microbiol 2014;117:485-97.
25. Saranya S, Hemashenpagam N. Purification and characterization of bacteriocin produced by different Lactobacillus species isolated from fermented foods. Int J MicroBiol Res 2013;5:341-8.
26. Dias FS, Duarte WF, Schwan RF. Evaluation of adhesive properties of presumptive probiotic Lactobacillus plantarum strains. Biosci J 2013;29:1678-86.
27. Doherty SB, Auty MA, Stanton C, Ross RP, Fitzgerald GF, Brodkorb A. Survival of entrapped Lactobacillus rhamnosus GG in whey protein micro-beads during simulated ex vivo gastro-intestinal transit. Int Dairy J 2012;22:31-43.
28. Gbassi GK, Vandamme T. Probiotic encapsulation technology: From microencapsulation to release into the gut. Pharmaceutics 2012;4:149-63.
29. Yusuf MA, Ichwan SJ, Hamid TH. Anti-proliferative activities of purified bacteriocin from Enterococcus mundtii strain c4l10 isolated from the caecum of malaysian non-broiler chicken on cancer cell lines. Int J Pharm Pharm Sci 2015;7:334-7.
30. Yusuf MA, Haziyamin T, Hamid A. Detection and characterization of enterocin encoding genes in Enterococcus mundtii strain c4l10 from the cecum of non-broiler chicken. Int J Pharm Pharm Sci 2015;7:131-6.
31. Szajewska H, Canani RB, Guarino A, Hojsak I, Indrio F, Kolacek S, et al. Probiotics for the prevention of antibiotic-associated diarrhea in children. J Pediatr Gastroenterol Nutr 2016;62:495-506.
32. Franz CMAP, Muscholl-Silberhorn AB, Yousif NMK, Vancanneyt M, Swings J, Holzapfel WH. Incidence of virulence factors and antibiotic resistance among enterococci isolated from food. Appl Environ Microbiol 2001. doi:10.1128/AEM.67.9.4385-4389.2001.
33. Tendolkar PM, Baghdayan AS, Shankar N. Putative surface proteins encoded within a novel transferable locus confer a high-biofilm phenotype to Enterococcus faecalis. J Bacteriol 2006;188:2063-72.
34. Alexandre Y, Le Berre R, Barbier G, Le Blay G. Screening of Lactobacillus spp. For the prevention of Pseudomonas aeruginosa pulmonary infections. BMC Microbiol 2014;14:107.
35. Kanmani P, Satish kumar R, Yuvaraj N, Paari KA, Pattukumar V, Arul V, et al. Production and purification of a novel exopolysaccharide from lactic acid bacterium Streptococcus phocae PI80 and its functional characteristics activity in vitro. Bioresour Technol 2011;102:4827-33.
36. Adlerberth I, Cerquetti M, Poilane I, Wold A, Collignon A. Mechanisms of colonisation and colonisation resistance of the digestive tract: Part 1: Bacteria/host interactions. Microb Ecol Health Dis 2000;12:223-39.
37. Balakrishna A. In vitro evaluation of adhesion and aggregation abilities of four potential probiotic strains isolated from guppy (Poecilia reticulata). Braz Arch Biol Technol 2013;56:793-800.
38. Ramos CL, Thorsen L, Schwan RF, Jespersen L. Strain-specific probiotics properties of Lactobacillus fermentum, Lactobacillus plantarum and Lactobacillus brevis isolates from Brazilian food products. Food Microbiol 2013;36:22-9.
39. Schär-Zammaretti P, Ubbink J. The cell wall of lactic acid bacteria: Surface constituents and macromolecular conformations. Biophys J 2003;85:4076-92.
40. Agboola FM. Probiotic potentials of lactic acid bacteria isolated from vaginal swabs on selected genital pathogens. Int J Pharm Sci Res 2014;5:2642-50.
41. Kheadr E, Dabour N, Le Lay C, Lacroix C, Fliss I. Antibiotic susceptibility profile of bifidobacteria as affected by oxgall, acid, and hydrogen peroxide stress. Antimicrob Agents Chemother 2007;51:169-74.
42. Favaro L, Basaglia M, Casella S, Hue I, Dousset X, Gombossy de Melo Franco BD, et al. Bacteriocinogenic potential and safety evaluation of non-starter Enterococcus faecium strains isolated from home made white brine cheese. Food Microbiol 2014;38:228-39.
43. Furtado DN, Todorov SD, Landgraf M, Destro MT, Franco BD. Bacteriocinogenic Lactococcus lactis subsp. Lactis DF04Mi isolated from goat milk: Evaluation of the probiotic potential. Braz J Microbiol 2014;45:1047-54.
44. Del Re B, Sgorbati B, Miglioli M, Palenzona D. Adhesion, autoaggregation and hydrophobicity of 13 strains of Bifidobacterium longum. Lett Appl Microbiol 2000;31:438-42.
45. Kos B, Suskovi? J, Vukovi? S, Simpraga M, Frece J, Matosi? S, et al. Adhesion and aggregation ability of probiotic strain Lactobacillus acidophilus M92. J Appl Microbiol 2003;94:981-7.
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ASHLESHA BHAGWAT, NANDANWAR Y S, RAHUL WARKE, and UDAY S ANNAPURE. “IN VITRO ASSESSMENT OF PHYSIOLOGICAL PROPERTIES OF ENTEROCOCCUS STRAINS OF HUMAN ORIGIN FOR POSSIBLE PROBIOTIC USE”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 6, Apr. 2019, pp. 194-03, https://innovareacademics.in/journals/index.php/ajpcr/article/view/33294.
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