GAS CHROMATOGRAPHY–MASS SPECTROSCOPY ANALYSIS OF ETHANOLIC EXTRACT OF LEAVES OF CORDIA OBLIQUA WILLD
Objective: Cordia obliqua Willd. also called as clammy cherry is a flowering plant species in the genus Cordia belonging to the family Boraginaceae. This study focus to find out the active principles and its therapeutic properties.
Methods: C. obliqua was collected from B. Maduvangarai, Tamil Nadu, India. The leaves were collected and extract prepared from ethanol by hot continuous percolation method in Soxhlet apparatus for 24 h. The ethanolic extract was collected and analyzed using Perkin–Elmer gas chromatography–mass spectroscopy (GC–MS) for the identification of active biochemical constituents present in the leaves of C. obliqua Willd.
Results: GC–MS analysis of the ethanolic extract of leaves of C. obliqua showed 11 bioactive compounds, the highest compound was named as 4-pentadecyne, 15-chloro-(13.574%) and lowest was named as 2-isopropyl-5-methylcyclohexyl 3-(1-(4-chlorophenyl)-3-oxobutyl)-coumarin-4-yl carbonate (6.461%).
Conclusion: In the present study, we concluded that the phytochemical constituents of the ethanolic extract of leaves of C. obliqua by GC–MS is to furnish the scientific information to evolve potential phytomedicine.
2. Gupta R, Gupta GD. A review on plant Cordia obliqua willd. (Clammy cherry). Pharmacogn Rev 2015;9:127-31.
3. Aswal BS, Goel AK, Kulshrestha DK, Mehrotra BN, Patnaik GK. Screening of Indian plants for biological activity: Part XV. Indian J Exp Biol 1996;34:444-67.
4. Abou-Shaaban RR, Al-Angari AA, El-Tahir KE, Al-Khamis KI, Mirghani OM. Comparative hypotensive and respiratory stimulation effects of ripe and unripe fruit mucilage of Cordia myxa and Cordia obliqua in guinea pigs and rabbits. Phytother Res 1989;3 Suppl 4:126 31.
5. Sertié JA, Basile AC, Panizza S, Matida AK, Zelnik R. Anti-inflammatory activity and sub-acute toxicity of artemetin. Planta Med 1990;56:36-40.
6. Tiwari RD, Srivastava KC, Shukla S, Bajpai RK. Chemical examination of the fixed oil from the seeds of Cordia myxa. Planta Med 1967;15:240 4.
7. Tiwari KP, Srivastava SS. Chemical investigation of the stem bark of Cordia obliqua. Planta Med 1979;36:191-2.
8. Udhayaprakash NK, Bhuvaneshwari S, Balamurugan A, Radhika B, Bhagya R, Sripriya N, et al. Studies on phytochemistry of 100 plants in Chennai, India. Br J Pharm Res 2013;3 Suppl 3:407-19.
9. Agnihotri VK, Srivastava SD, Srivastava SK, Pitre S, Rusia K. Constituents from the seeds of Cordia obliqua as potential anti-inflammatory agents. Indian J Pharm Sci 1987;49:66-9.
10. Yadav R, Yadav SK. Evaluation of antimicrobial activity of seeds and leaves of Cordia obliqua Willd against some oral pathogens. Indo Am J Pharm Res 2013;3:6035-43.
11. Arinathan V, Mohan VR, De Britto AJ, Murugan C. Wild edibles used by palliyars of the Western Ghats, Tamil Nadu. Indian J Tradit Knowl 2007;6 Suppl 1:163-8.
12. Parmar C, Kaushal MK, editors. Cordia obliqua. In: Wild Fruits of the Sub-Himalayan Region. New Delhi: Kalyani Publishers; 1982. p. 136.
13. Uphof JC. Dictionary of Economic Plants. 2nd ed., Vol. 162. New York: Cramer, Wiirzburg Science; 1968. p. 890.
14. Baveja SK, Rao KV, Arora J. Examination of natural gums and mucilages as sustaining materials in tablet dosage forms. Indian J Pharm Sci 1988;50:89-92.
15. Watt G. Dictionary of the Economic Products of India. Calcutta: Department of Revenue and Agriculture, Government of India; 1889.
16. Kritikar KR, Basu BD. Indian Medicinal Plants. Dehradun: Shiva Offset Press; 1998. p. 1674-81.
17. Thirupathi K, Kumar SS, Raju VS, Ravikumar B, Krishna DR, Mohan GK. A review of medicinal plants of the genus cordia: Their chemistry and pharmacological uses. J Nat Remedies 2008;8 Suppl 1:1 10.
18. Sivakrishnan S, Kottaimuthu A. Hepatoprotective activity of ethanolic extract of aerial parts of Albizia procera Roxb (Benth.) against paracetamol induced liver toxicity on wistar rats. Int J Pharm Pharm Sci 2014;6 Suppl 1:233-8.
19. Sivakrishnan S, Kottaimuthu A, Kavitha J. GC-MS analysis of ethanolic extract of aerial parts of Albizia procera (Roxb) Benth. Int J Pharm Pharm Sci 2013;5 Suppl 3:702-4.
20. Sivakrishnan S, Kottaimuthu A, Kavitha J. Antioxidant potential, total phenolic and flavonoids content of aerial parts of ethanolic extract of Albizia procera. Asian J Pharm Clin Res 2013;6 Suppl 1:108-10.
21. Ezhilan BP, Neelamegam R. GC-MS analysis of phytocomponents in the ethanol extract of Polygonum chinense L. Pharmacogn Res 2012;4 Suppl 1:11-4.
22. Mass Spectrometry Data Centre. Eight Peak Index of Mass Spectra: The Eight Most Abundant Ions in 31,101 Mass Spectra, indexed by Molecular Weight, Elemental Composition and Most Abundant Ion (4 volume set). 2nd ed. Alder Maston: Mass Spectrometry Data Centre; 1974.
23. Uduman MS, Rathinam P, Karuru Y, Obili G, Chakka G, Janakiraman AK. GC-MS analysis of ethyl acetate extract of whole plant of Rostellularia diffusa. Pharmacogn J 2017;9 Suppl 1:70-2.
24. Patel J, Reddy V, Kumar GS, Satyasai D, Bajari B. Gas chromatography and mass spectroscopy analysis of bioactive components on the leaf extract of Terminalia coriacea: A potential folklore medicinal plant. Int J Green Pharm 2017;11 Suppl 1:140-3.
25. Sermakkani M, Thangapandian V. GC-MS analysis Cassia italica leaf methanol extract. Asian J Pharm Clin Res 2012;5 Suppl 2:90-4.
26. Sultana S, Nagarajan B. Simultaneous trace level determination of benzene and 1,2-dichloroethane by GC-HS/GC-MS in several pharmaceutical substances. Int J Appl Pharm 2019;11 Suppl 1:82-8.
27. Ashok K, Jayaprakash P. Screening of active phytocompounds by GC-MS study and antimicrobial activity in the stem of Santalum album. Int J Curr Pharm Res 2012;4 Suppl 3:43-4.
28. Bell J. Antioxidant nutrition: An adjuvant with a balance diet. J Crit Rev 2018;5 Suppl 1:4.
29. Laveena KB, Chandra M. Evaluation of bioactive compounds, antioxidant and antibacterial properties of medicinal plants Sauropus androgynus L. and Erythrina variegata L. Asian J Pharm Clin Res 2018;11 Suppl 12:313-7.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.