ISOLATION AND IN VITRO ANTIOXIDANT ACTIVITY OF FLAVONOID FROM LINDERNIA CRUSTACEA (L) F. MUELL

SMRITI REKHA CHANDA DAS; ABDUL BAQUEE AHMED; DIBYENDU SHIL; INDRANIL CHANDA

doi:10.22159/ajpcr.2019.v12i6.33344

Authors

SMRITI REKHA CHANDA DAS Department of Pharmacy, Girijananda Chowdhury Institute of Pharmaceutical Science, Guwahati, Assam, India.
ABDUL BAQUEE AHMED Department of Pharmacy, Girijananda Chowdhury Institute of Pharmaceutical Science, Guwahati, Assam, India.
DIBYENDU SHIL Department of Pharmacy, Girijananda Chowdhury Institute of Pharmaceutical Science, Guwahati, Assam, India.
INDRANIL CHANDA Department of Examinations, Assam Science and Technology University, Guwahati, Assam, India.

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i6.33344

Keywords:

Lindernia crustacea (L) F Muell,, Flash chromatography,, Flavonoid,, Antioxidant activity,, IC50 value

Abstract

Objective: The objective of the study was to investigate the antioxidant property of different extracts of Lindernia crustacea (L) F. Muell and isolate flavonoid from the potent extract and characterize it.

Methods: Isolation was carried out by flash chromatography using Toluene:acetic acid (4:1) as eluent. The isolated compound was characterized using spectroscopic methods. 2, 2′- diphenyl-1-picrylhydrazyl, ferric thiocyanate, thiobarbituric acid, and reducing power assay methods were followed for the antioxidant study.

Results: Characterization of the isolated compound confirms it as the flavonoid. Results of the antioxidant study showed that benzene extract has the highest antioxidant activity with a less IC50 value in comparison to ethyl acetate and ethanol extracts. The isolated compound showed significant antioxidant activity when compared with aspirin.

Conclusion: The results of the study suggest that L. crustacea (L) F. Muell is a source of flavonoid which has potent antioxidant activity.

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