ISOLATION AND IN VITRO ANTIOXIDANT ACTIVITY OF FLAVONOID FROM LINDERNIA CRUSTACEA (L) F. MUELL
Keywords:Lindernia crustacea (L) F Muell,, Flash chromatography,, Flavonoid,, Antioxidant activity,, IC50 value
Objective: The objective of the study was to investigate the antioxidant property of different extracts of Lindernia crustacea (L) F. Muell and isolate flavonoid from the potent extract and characterize it.
Methods: Isolation was carried out by flash chromatography using Toluene:acetic acid (4:1) as eluent. The isolated compound was characterized using spectroscopic methods. 2, 2′- diphenyl-1-picrylhydrazyl, ferric thiocyanate, thiobarbituric acid, and reducing power assay methods were followed for the antioxidant study.
Results: Characterization of the isolated compound confirms it as the flavonoid. Results of the antioxidant study showed that benzene extract has the highest antioxidant activity with a less IC50 value in comparison to ethyl acetate and ethanol extracts. The isolated compound showed significant antioxidant activity when compared with aspirin.
Conclusion: The results of the study suggest that L. crustacea (L) F. Muell is a source of flavonoid which has potent antioxidant activity.
Halliwell B, Gutteridge JM. Free Radicals in Biology and Medicine. 3rd ed. United Kingdom: Oxford University Press; 1999.
Rakesh SU, Priyanka RP, Sagar RM. Use of natural antioxidants to scavenge free radicals: A major cause of diseases. Int J Pharm Tech Res 2010;2:1074-81.
Kapadiya DB, Dabhi BK, Aparnathi KD. Spices and herbs as a source of natural antioxidants for food. Int J Curr Microbiol Appl Sci 2016;5:280-8.
Panche AN, Diwan AD, Chandra SR. Flavonoids: An overview. J Nutri Sci 2016;5:e47:1-15.
Ekor M. The growing use of herbal medicines: Issues relating to adverse reactions and challenges in monitoring safety. Front Pharmacol 2014;4:177.
Afsar T, Razak S, Shabbir M, Khan MR. Antioxidant activity of polyphenolic compounds isolated from ethyl-acetate fraction of Acacia hydaspica R. Parker. Chem Cent J 2018;12:5.
Anonymous. The Wealth of India-Raw Materials. New Delhi, India: Council of Scientific and Industrial Research; 2005.
Usher G. A Dictionary of Plants used by Man. London: Constable and Company Ltd.; 1974.
Chanda SR, Ahmed AB, Saha D, Chanda I. Scientific evidence of Lindernia crustacea (L) F. Muell, an indigenous plant: A folklore medicine used traditionally. Int Res J Pharm 2019;10:176-83.
Trease GE, Evans WC. Pharmacognosy. Oxford: Alden Press; 1996.
Wagner H, Bladt S, Zgainski EM. Plant Drug Analysis: A Thin-Layer Chromatography Atlas. Heidelberg: Springer-Verlag; 1996.
Brand-Williams W, Cuvelier ME, Berset C. Use of the free radical method to evaluate antioxidant activity. LWT 1995;28:25-30.
Bokhari J, Khan MR, Haq IU. Assessment of phytochemicals, antioxidant, and anti-inflammatory potential of Boerhavia procumbens banks ex Roxb. Toxicol Ind Health 2016;32:1456-66.
Zhao T, Zhang Q, Qi H, Zhang H, Niu X, Xu Z, et al. Degradation of porphyran from Porphyra haitanensis and the antioxidant activities of the degraded porphyrans with different molecular weight. Int J Biol Macromol 2006;38:45-50.
Kikuzaki H, Nakatani N. Antioxidant effect of some ginger constituents. J Food Sci 1993;58:1407-10.
Oyaizu M. Studies on the product of browning reaction prepared from glucose amine. Jpn J Nutr 1986;44:307-15.
Mu H, Zhang A, Zhang W, Cui G, Wang S, Duan J, et al. Antioxidative properties of crude polysaccharides from Inonotus obliquus. Int J Mol Sci 2012;13:9194-206.
Wang J, Gao H, Zhao J, Wang Q, Zhou L, Han J, et al. Preparative separation of phenolic compounds from Halimodendron halodendron by high-speed counter-current chromatography. Molecules 2010;15:5998-6007.
Zhu ZJ, Pan R, Si JY, Fu Y, Huang QQ. Study on the chemical constituents of Bupleurum bicaule Helm (in Chinese with English abstract). Nat Prod Res Dev 2008;20:833-5.
Bharathi S, Devasena T, Arivarasan A, Pachaiappan R. Extraction and isolation of flavonoid quercetin from the leaves of Trigonella foenum-graecum and their anti-oxidant activity. Int J Pharm Pharm Sci 2016;8:120-4.
Chourasiya A, Upadhayay A, Shukla RN. Isolation of quercetin from the leaves of Azadirachta indica and antidiabetic study of the crude extracts. J Pharm Biomed Sci 2012;25:179-81.
Mabry TJ, Markham KR, Thomas MB. The Systematic Identification of Flavonoids. New York: Springer Verlag; 1970.
Kurechi T, Kikugawa K, Kato T. Studies on the antioxidants. XIII. Hydrogen donating capability of antioxidants to 2, 2-diphenyl-1- picrylhydrazyl. Chem Pharm Bull 1980;28:2089-93.
Nounagnon MS, Dah-nouvlessounon D, N’Tcha C, Legba B, Baba-moussa F, Adjanohoun A, et al. Phytochemistry and biological activities of Crateva adansonii extracts. Int J Pharm Pharm Sci 2018;10:62-7.
Sumita D, Maitry P, Nirali P. Antioxidant activities of some less utilized edible fruits. Int J Curr Pharm Res 2017;9:28-31.
Phongpaichit S, Nikom J, Rungjindamai N, Sakayaroj J, Hutadilok- Towatana N, Rukachaisirikul V, et al. Biological activities of extracts from endophytic fungi isolated from Garcinia plants. FEMS Immunol Med Microbiol 2007;51:517-25.
Renuka K, Devi VR, Subramanian SP. Phytochemical screening and evaluation of in vitro antioxidant potential of immature Palmyra Palm (Borassus flabellifer Linn.) Fruits. Int J Pharm Pharm Sci 2018;10:77-83.
Saha K, Lajis NH, Israf DA, Hamzah AS, Khozirah S, Khamis S, et al. Evaluation of antioxidant and nitric oxide inhibitory activities of selected Malaysian medicinal plants. J Ethnopharmacol 2004;92:263-7.
Rahmat A, Kumar V, Fong LM, Endrini S, Sani HA. Determination of total antioxidant activity in three types of local vegetables shoots and the cytotoxic effect of their ethanolic extracts against different cancer cell lines. Asia Pac J Clin Nutr 2003;12:292-5.
Aiyegoro OA, Okoh AI. Preliminary phytochemical screening and in vitro antioxidant activities of the aqueous extract of Helichrysum longifolium DC. BMC Complement Altern Med 2010;10:21.
Rezaeizadeh A, Zuki AB, Abdollahi M, Goh YM, Noordin MM, Hamid M, et al. Determination of antioxidant activity in methanolic and chloroformic extracts of Momordica charantia. Afr J Biotechnol 2011;10:4932-40.
Umamaheswari M, Chatterjee TK. In vitro antioxidant activities of the fractions of Coccinia grandis L. Leaf extract. Afr J Tradit Complement Altern Med 2007;5:61-73.
Dorman HJ, Peltoketo A, Hiltunen R, Tikkanen MJ. Characterisation of the antioxidant properties of deodourisation aqueous extracts from selected Lamiaceae herbs. Food Chem 2003;83:255-6.
Yildirim A, Mavi A, Oktay M, Kara AA, Algur OF, Bilaloglu V, et al. Comparison of antioxidant and antimicrobial activities of Tilia (Tilia argentea Desf ex DC), sage (Salvia triloba l.), and black tea (Camellia sinensis) extracts. J Agric Food Chem 2000;48:5030-4.
Duh PD. Antioxidant activity of burdock (Arctium lappa Linne.): Its scavenging effect on free-radical and active oxygen. J Am Oil Chem Soc 1998;75:455-61.
Duh PD, Du PC, Yen GC. The action of methanolic extract of mung beans hulls as inhibitors of lipid peroxidation and non-lipid oxidative damage. Food Chem Toxicol 1999;37:1055-61.
Steenkamp V, Stewart MJ, Chimuka L, Cukrowska E. Uranium concentrations in South African herbal remedies. Health Phys 2005;89:679-83.
How to Cite
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.