PRELIMINARY PHYTOCHEMICAL SCREENING AND GAS CHROMATOGRAPHY AND MASS SPECTROMETRY ANALYSIS OF CHLOROFORM EXTRACT OF GARCINIA CAMBOGIA FRUITS
Keywords:Garcinia cambogia,, Bioactive compounds,, Gas chromatography and mass spectrometry analysis analysis
Objective: Garcinia cambogia fruit commonly known as Malabar tamarind, belongs to the family Clusiaceae, is said to show varied medicinal effects including antiobesity, antiviral, antimicrobial, anti-inflammatory, and anticancer. Hence, the present study deals with the determination of phytochemical constituents present in the chloroform extract of G. cambogia fruits and determination of bioactive compounds using the gas chromatography and mass spectrometry analysis (GC-MS) technique.
Methods: The phytochemical constituents present in the chloroform extract of G. cambogia fruits were tested by qualitative analysis, and bioactive compounds were investigated using the GC-MS technique. The analysis was carried out on a JOEL GC MATERI system with a column packed with a column HP5MS (5% phenyl, methylpolysiloxane) fused capillary column (30 × 0.25 μm ID × 0.25 μm df). The compounds are separated using helium as a carrier gas at a constant flow 1 ml/min. The mass spectra of the known compounds in the fruit extract were matched with the known components stored in the National Institute of Standard and technology library.
Results: The phytochemical test revealed the presence of xanthones, flavonoids, phlobatannins, terpenoids steroids, phenolic compounds saponins, and anthraquinones. The GC-MS analysis provided peaks of 13 different bioactive compounds, namely 3,7,11,15-tetramethyl-2-hexadecenol (37.1%), pentadecanoic acid 14-methyl-, methylester (100%), hexadecanoic acid 15 methyl-, methyl ester (100%), 9,12-octadecadienoic acid methyl ester (42.6%), docosanoic acid (100%), docosanoic acid methyl ester (37.3%), phenol, 2,4,bis1,1-dimethylethyl) (100%), 9, hexadecanoic acid methyl ester (99.2%), 10-octadecenoic acid methylester (100%), 11 eicosenoic acid methyl ester (53.1%), heptadecanoic acid 9 methyl-, methyl ester (100%), eicosenoic acid methyl ester (56.9%), and 5, 12d ethanofuro oxepino[2,3,4-mn] (2,3,4-ed) anthrace2-one 9,12-dilol 6 methyl,2a 3,4,4a,5,6,7,8a‑octahydro (19%) were reported.
Conclusion: The phytochemical investigation and determination of bioactive compounds will be considered for pharmacological activities, and further isolation of individual components would however help to find new drugs.
Sudhakar M, Bolkiah R. Phytochemical determination of polyherbal extract using FTIR and Gc–MS analysis. Eur J Pharm Res 2015;5:173 8.
Das TS, Ramesh L, Agastian P. Gc–MS analysis of bioactive constituents of Aristolochia bracteolata Linn with in vitro antioxidant properties. J Appl Pharm Sci 2016;6:61-8.
Krishnaiah GM, Prashanth GK. Phytochemical studies, and GC–MS analysis of the leaf extracts of Melia azedarach Linn. Int J Adv Eng Technol Manage Appl Sci 2014;1:48-54.
Anand D, Johnson W, Saravanan P, Rajarajan S. Phytochemical analysis of leaf extract Eclipta alba (L.) Hassk by GC-MS method. Int J Pharmacogn Phytochem Res 2014;6:562-6.
Rukshana MS, Dossa A, Rani TP. Phytochemical screening and GC-MS analysis of leaf extract of Pergularia daemia (Forssk) (Chiov). Asian J Plant Sci Res 2017;7:9-15.
Ammal RM, Bai GV. GC-MS determination of bioactive constituents of Heliotropium indicum leaf. J Med Plant Stud 2013;1:30-3.
Komalavalli T, Lincy P, Kumarasamy SM, Mohan VR. Determination of bioactive components of anastasia Travancorica Bedd (acanthaceae) by GC-MS analysis. Int J Pharm Clin Res 2014;6:155-8.
Irulandi K, Anatharaj S, Selvan T, Mehalingan P. GC-MS analysis and phytochemical studies of methanolic fruits extract of Garcinia cambogia Hart. EX Boerl and Ziziphus trinervia Roth. J Adv Appl Sci Res 2016; 2:90-5.
Choppa T, Selvaraj CI, Zachariah A. Evaluation and characterization of Malabar tamarind [Garcinia cambogia (Gaertn.) Desr.] seed oil. J Food Sci Technol 2015;52:5906-13.
Jamila N, Choi JY, Hong JH, Nho EY, Khan N, Jo CH, et al. Identification and quantification of adulteration in Garcinia cambogia commercial products by chromatographic and spectrometric methods. Food Addit Contam Part A Chem Anal Control Expo Risk Assess 2016;33:1751-60.
Vardharaj V, Kupan M. Identification and determination of bioactive phytochemical constituents from the hydroalcholic extract of Achyranthes aspera whole plant by gas chromatography–mass spectrometry analysis. Asian J Pharm Clin Res 2015;8:125-9.
Harborne JB. Phytochemical Methods. London: Chapman and Hall, Ltd.; 1973. p. 49-88.
Krishnamoorthy V, Nagappan P, Serene AK, Rajendran R. Preliminary phytochemical screening of the fruit rind of Garcinia cambogia and leaves of Bauhinia variegata comparative study. Int J Curr Microbiol Appl Sci 2014;3:479-86.
Sahani K, Thakur D. GC-MS analysis of endophytic fungi Curvularia aeria MTCC12847 isolated from Tribulus terrestris L. Int J Pharm Pharm Sci 2019;11:26-32.
Chidambaram V, Niraimathi L, Sudha V, Lavanya R, Vadivel V, Brindha P. Spectrophotometric, HPTLC, and GC-MS studies on selected spice extracts. Int J Pharm Pharm Sci 2015;7:184-90.
Kalimuthu K, Prabakaran R. Preliminary phytochemical screening and GC-MS analysis of methanol extract of Ceropegia pusilla. Int J Res Appl Nat Soc Sci 2013;1:49-58.
Selvamangai C, Bhaskar A. GC-MS analysis of phytocomponents in the methanolic extract of Eupatorium triplinerve. Int J Drug Dev 2010;4:148-53.
Selvakumar S, Valliammai R. Determination of bioactive and pharmaceutical components of Croton boripladianus by GC–MS analysis. Int J Pharm Sci Nanotechnol 2016;9:1-6.
Gupta AK, Bhat JL. GC-MS analysis of methanol extract of Acacia nilotica (L.) Leaves. Int J Pharm Chem 2016;6:50-3.
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