Biological synthesis of AgNPs BIOLOGICAL SYNTHESIS OF SILVER NANOPARTICLES USING THE TUBEROUS ROOT EXTRACT OF IPOMOEA BATATAS AND THEIR CHARACTERIZATIONS AND ANTIBACTERIAL ACTIVITY
Biological synthesis of AgNPs
Objectives: Tuberous root extract based synthesis of silver nanoparticles (AgNPs), characterizations using Fourier-transform infrared spectroscopy (FT-IR), UV-visible, powder X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), energy dispersive X-ray (EDX) techniques, and antibacterial activity of AgNPs against Staphylococcus aureus, Staphylococcus mutans, Proteus vulgaris, and Escherichia coli.
Methods: Root extract of Ipomoea batatas (sweet potato) was prepared by boiling of small cut pieces of root with double distilled water. Added root extract with silver nitrate solution and centrifuged and collect the pellets. After several washing and drying, AgNPs have been preserved for characterizations and antibacterial activity.
Results: The synthesized AgNPs were well characterized by FT-IR, UV-visible, XRD, FESEM, and EDX methods, and significant zones of inhibition observed around the loaded AgNPs on the agar plates. The zones of inhibition have been achieved 36, 40, 46, and 32 mm for E. coli, P. vulgaris, S. mutans, and S. aureus.
Conclusion: The characterisation methods such as UV-Visible, FTIR, Powder XRD, FESEM and EDX indicate an efficient formation of AgNPs using root extract of I. batatas. The biologically synthesized AgNPs are found good antibacterial agents.
2. Raj LF, Jayalakshmy E. A biogenic approach for the synthesis and characterization of zinc oxide nanoparticles produced by Tinospora cordifolia. Intern J Pharm Pharm Sci 2015;8:384-6.
3. Menon S, Agarwal H, Kumar SR, Kumar SV. Green synthesis of silver nanoparticles using medicinal plant Acalypha indica leaf extracts and its application as an antioxidant and antimicrobial agent against foodborne pathogens. Intern J Appl Pharma 2017;9:42-50.
4. Anbarasu A, Karnan P, Deepa N, Usha R. Carica papaya mediated green synthesized silver nanoparticles. Intern J Current Pharma Res 2018;10:15-20.
5. Albanese A, Tang PS, Chan WC. The effect of nanoparticle size, shape, and surface chemistry on biological systems. Annu Rev Biomed Eng 2012;14:1-6.
6. Arvizo RR, Bhattacharyya S, Kudgus RA, Giri K, Bhattacharya R, Mukherjee P, et al. Intrinsic therapeutic applications of noble metal nanoparticles: Past, present and future. Chem Soc Rev 2012;41:2943 70.
7. Anandalakshmi K, Venugobal J, Ramasamy V. Characterization of silver nanoparticles by green synthesis method using Pedalium murex leaf extract and their antibacterial activity. Appl Nanosci 2017;6:399 408.
8. Behravan M, Hossein Panahi A, Naghizadeh A, Ziaee M, Mahdavi R, Mirzapour A, et al. Facile green synthesis of silver nanoparticles using Berberis vulgaris leaf and root aqueous extract and its antibacterial activity. Int J Biol Macromol 2019;124:148-54.
9. Sathishkumar M, Sneha K, Won SW, Cho CW, Kim S, Yun YS, et al. Cinnamon zeylanicum bark extract and powder mediated green synthesis of nano-crystalline silver particles and its bactericidal activity. Colloids Surf B Biointerfaces 2009;73:332-8.
10. Gan L, Zhang S, Zhang Y, He S, Tian Y. Biosynthesis, characterization and antimicrobial activity of silver nanoparticles by a halotolerant Bacillus endophyticus SCU-L. Prep Biochem Biotechnol 2018;5:1-7.
11. Lin PC, Lin S, Wang PC, Sridhar R. Techniques for physicochemical characterization of nanomaterials. Biotechnol Adv 2014;32:711-26.
12. Pleus R. Nanotechnologies-Guidance on Physicochemical Characterization of Engineered Nanoscale Materials for Toxicologic Assessment. Geneva, Switzerland: ISO; 2012.
13. Carlson C, Hussain SM, Schrand AM, Braydich-Stolle LK, Hess KL, Jones RL, et al. Unique cellular interaction of silver nanoparticles: Size-dependent generation of reactive oxygen species. J Phys Chem B 2008;112:13608-19.
14. Jo DH, Kim JH, Lee TG, Kim JH. Size, surface charge, and shape determine therapeutic effects of nanoparticles on brain and retinal diseases. Nanomedicine 2015;11:1603-11.
15. Staquicini FI, Ozawa MG, Moya CA, Driessen WH, Barbu EM, Nishimori H, et al. Systemic combinatorial peptide selection yields a non-canonical iron-mimicry mechanism for targeting tumors in a mouse model of human glioblastoma. J Clin Invest 2011;121:161-73.
16. Duan X, Li Y. Physicochemical characteristics of nanoparticles affect circulation, biodistribution, cellular internalization, and trafficking. Small 2013;9:1521-32.
17. Park SY, Lee SY, Yang JW, Lee JS, Oh SD, Oh S, et al. Comparative analysis of phytochemicals and polar metabolites from colored sweet potato (Ipomoea batatas L.) tubers. Food Sci Biotechnol 2016;25:283 91.
18. Majid M, Nasir B, Zahra SS, Khan MR, Mirza B, Haq I. Ipomoea batatas L. Lam. ameliorates acute and chronic inflammations by suppressing inflammatory mediators, a comprehensive exploration using in vitro and in vivo models. BMC Complement Altern Med 2018;18:216.
19. Wypij M, Czarnecka J, ?wiecimska M, Dahm H, Rai M, Golinska P, et al. Synthesis, characterization and evaluation of antimicrobial and cytotoxic activities of biogenic silver nanoparticles synthesized from Streptomyces xinghaiensis OF1 strain. World J Microbiol Biotechnol 2018;34:23.
20. Siddiqi KS, Husen A, Rao RAK. A review on biosynthesis of silver nanoparticles and their biocidal properties. J Nanobiotechnology 2018;16:14-42.
21. Siddiqi KS, Husen A, Rao RAK. A review on biosynthesis of silver nanoparticles and their biocidal properties. J Nanobiotechnology 2018;16:14-42.
22. Joshi NC, Singh A, Rajput H. Utilization of waste leaves biomass of Myrica esculenta for the removal of Pb (II), Cd(II) and Zn(II) ions from waste waters. Orient J Chem 2018;34:2548-53.
23. Chen S, Kimura K. Synthesis of thiolate-stabilized platinum nanoparticles in protolytic solvents as isolable colloids, J Phys Chem B 2001;105:5397-403.
24. Pirtarighat S, Ghannadnia M, Baghshahi S. Green synthesis of silver nanoparticles using the plant extract of Salvia spinosa grown in vitro and their antibacterial activity assessment. J Nanostruct Chem 2019;9:1-9.
25. Elamawi RM, Al-Harbi RE, Hendi AA. Biosynthesis and characterization of silver nanoparticles using Trichoderma longibrachiatum and their effect on phytopathogenic fungi. Egypt J Biol Pest Control 2018;28:28 39.
26. Young KJ, Byung HK, Geunhwa J. Antifungal activity of silver ions and nanoparticles on phytopathogenic fungi. Plant Dis 2009;93:1037 43.
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