• PRITAM GOSWAMI Department of Pathology, Mahesh Bhattacharyya Homoeopathic Medical College and Hospital, Howrah, West Bengal, India.
  • SWAIF ALI SK Department of Pathology, Mahesh Bhattacharyya Homoeopathic Medical College and Hospital, Howrah, West Bengal, India.
  • SAYAK GHOSH Department of Pathology, Mahesh Bhattacharyya Homoeopathic Medical College and Hospital, Howrah, West Bengal, India.
  • ANAMIKA BASU Department of Pathology, Mahesh Bhattacharyya Homoeopathic Medical College and Hospital, Howrah, West Bengal, India.
  • JOYDEEP KHANRA Department of Pathology, Mahesh Bhattacharyya Homoeopathic Medical College and Hospital, Howrah, West Bengal, India.
  • SATADAL DAS Department of Microbiology, Peerless Hospital and B K Roy Research Centre, Kolkata, West Bengal, India.


Objectives: Since its discovery, the role of histamine in inflammation is controversial; thus, according to some authority, it is mainly pro-inflammatory, and according to others, it is anti-inflammatory in nature. In this scenario, we thought that the contradictory results are dose dependent, thus in this study, our aim was to find the specific role of ultra-diluted histamine in pulmonary inflammation.

Materials and Methods: Ultra-diluted histamine ( ~1 pg/ml) was administered in chick lung hypoxic inflammation in an restricted organoid culture along with lysozyme, ovalbumin, and blank controls.

Results: The ultra-diluted histamine showed a significant role as an anti-inflammatory and bronchodilator agent and the anti-inflammatory action was found similar to lysozyme.

Conclusion: Ultra-diluted histamine may be used as an anti-inflammatory agent.

Keywords: Ultra-diluted histamine,, Anti-inflammatory agent,, Ovalbumin,, Lysozyme,, Hypoxia.

Author Biography

SATADAL DAS, Department of Microbiology, Peerless Hospital and B K Roy Research Centre, Kolkata, West Bengal, India.

Principal Investigator,

Virology Laboratory


1. Linde K, Clausius N, Ramirez G, Melchart D, Eitel F, Hedges LV, et al. Are the clinical effects of homeopathy placebo effects? A meta-analysis of placebo-controlled trials. Lancet 1997;350:834-43.2. Lichtenstein LM, Gillespie E. The effects of the H1 and H2 antihistamines on “allergic” histamine release and its inhibition by histamine. J Pharmacol Exp Ther 1975;192:441-50.
3. Masini E, Blandina P, Brunelleschi S, Mannaioni PF. Evidence for H2- receptor-mediated inhibition of histamine release from isolated rat mast cells. Agents Actions 1982;12:85-8.
4. Sainte-Laudy J, Belon P. Inhibition of human basophil activation by high dilutions of histamine. Agents Actions 1993;38:C245-7.
5. Sainte-Laudy J, Belon P. Analysis of immunosuppressive activity of serial dilutions of histamine on human basophil activation by flow cytometry. Inflamm Res 1996;45 Suppl 1:S33-4.
6. Vannier E, Dinarello CA. Histamine enhances interleukin (IL)- 1-induced IL-1 gene expression and protein synthesis via H2 receptors in peripheral blood mononuclear cells. Comparison with IL-1 receptor antagonist. J Clin Invest 1993;92:281-7.
7. Merétey K, Falus A, Taga T, Kishimoto T. Histamine influences the expression of the interleukin-6 receptor on human lymphoid, monocytoid and hepatoma cell lines. Agents Actions 1991;33:189-91.
8. Jeannin P, Delneste Y, Gosset P, Molet S, Lassalle P, Hamid Q, et al. Histamine induces interleukin-8 secretion by endothelial cells. Blood 1994;84:2229-33.
9. Bayram H, Devalia JL, Khair OA, Abdelaziz MM, Sapsford RJ, Czarlewski W, et al. Effect of loratadine on nitrogen dioxide-induced changes in electrical resistance and release of inflammatory mediators from cultured human bronchial epithelial cells. J Allergy Clin Immunol 1999;104:93-9.
10. Nakamura T, Itadani H, Hidaka Y, Ohta M, Tanaka K. Molecular cloning and characterization of a new human histamine receptor, HH4R. Biochem Biophys Res Commun 2000;279:615-20.
11. Zhu Y, Michalovich D, Wu H, Tan KB, Dytko GM, Mannan IJ, et al. Cloning, expression, and pharmacological characterization of a novel human histamine receptor. Mol Pharmacol 2001;59:434-41.
12. Schaefer U, Schmitz V, Schneider A, Neugebauer E. Histamine induced homologous and heterologous regulation of histamine receptor subtype mRNA expression in cultured endothelial cells. Shock 1999;12:309-15.
13. O’Reilly M, Alpert R, Jenkinson S, Gladue RP, Foo S, Trim S, et al. Identification of a histamine H4 receptor on human eosinophils role in eosinophil chemotaxis. J Recept Signal Transduct Res 2002;22:431-48.
14. Brown V, Ennis M. Flow-cytometric analysis of basophil activation: Inhibition by histamine at conventional and homeopathic concentrations. Inflamm Res 2001;50 Suppl 2:S47-8.
15. Sainte-Laudy J, Belon P. Application of flow cytometry to the analysis of the immunosuppressive effect of histamine dilutions on human basophil activation: Effect of cimetidine. Inflamm Res 1997;46 Suppl 1:S27-8.
16. Hill DJ, Hosking CS, de Benedictis FM, Oranje AP, Diepgen TL, Bauchau V, et al. Confirmation of the association between high levels of immunoglobulin E food sensitization and eczema in infancy: An international study. Clin Exp Allergy 2008;38:161-8.
17. Bernhisel-Broadbent J, Dintzis HM, Dintzis RZ, Sampson HA. Allergenicity and antigenicity of chicken egg ovomucoid (Gal d III) compared with ovalbumin (Gal d I) in children with egg allergy and in mice. J Allergy Clin Immunol 1994;93:1047-59.
18. Miller H, Campbell DH. Skin test reactions to various chemical fractions of egg white and their possible clinical significance. J Allergy 1950;21:522-4.
19. Bleumink E, Young E. Studies on the atopic allergen in hen’s egg. II. Further characterization of the skin-reactive fraction in egg-white; immuno-electrophoretic studies. Int Arch Allergy Appl Immunol 1971;40:72-88.
20. Cooke SK, Sampson HA. Allergenic properties of ovomucoid in man. J Immunol 1997;159:2026-32.
21. Heine RG, Laske N, Hill DJ. The diagnosis and management of egg allergy. Curr Allergy Asthma Rep 2006;6:145-52.
22. Astwood JD, Leach JN, Fuchs RL. Stability of food allergens to digestion in vitro. Nat Biotechnol 1996;14:1269-73.
23. Vitiello L, Gorini S, Rosano G, la Sala A. Immunoregulation through extracellular nucleotides. Blood 2012;120:511-8.
24. Eltzschig HK, Sitkovsky MV, Robson SC. Purinergic signaling during inflammation. N Engl J Med 2012;367:2322-33.
25. Müller T, Vieira RP, Grimm M, Dürk T, Cicko S, Zeiser R, et al. A potential role for P2X7R in allergic airway inflammation in mice and humans. Am J Respir Cell Mol Biol 2011;44:456-64.
26. Müller T, Robaye B, Vieira RP, Ferrari D, Grimm M, Jakob T, et al. The purinergic receptor P2Y2 receptor mediates chemotaxis of dendritic cells and eosinophils in allergic lung inflammation. Allergy 2010;65:1545-53.
27. Stachon P, Peikert A, Michel NA, Hergeth S, Marchini T, Wolf D, et al. P2Y6 deficiency limits vascular inflammation and atherosclerosis in mice. Arterioscler Thromb Vasc Biol 2014;34:2237-45.
28. Vieira RP, Müller T, Grimm M, von Gernler V, Vetter B, Dürk T, et al. Purinergic receptor type 6 contributes to airway inflammation and remodeling in experimental allergic airway inflammation. Am J Respir Crit Care Med 2011;184:215-23.
29. Liu GD, Ding JQ, Xiao Q, Chen SD. P2Y6 receptor and immunoinflammation. Neurosci Bull 2009;25:161-4.
30. Ben Yebdri F, Kukulski F, Tremblay A, Sévigny J. Concomitant activation of P2Y(2) and P2Y(6) receptors on monocytes is required for TLR1/2-induced neutrophil migration by regulating IL-8 secretion. Eur J Immunol 2009;39:2885-94.
31. Zhang Z, Wang Z, Ren H, Yue M, Huang K, Gu H, et al. P2Y(6) agonist uridine 5’-diphosphate promotes host defense against bacterial infection via monocyte chemoattractant protein-1-mediated monocytes/ macrophages recruitment. J Immunol 2011;186:5376-87.
32. Idzko M, la Sala A, Ferrari D, Panther E, Herouy Y, Dichmann S, et al. Expression and function of histamine receptors in human monocyte- derived dendritic cells. J Allergy Clin Immunol 2002;109:839-46.
33. Gutzmer R, Langer K, Lisewski M, Mommert S, Rieckborn D, Kapp A, et al. Expression and function of histamine receptors 1 and 2 on human monocyte-derived dendritic cells. J Allergy Clin Immunol 2002;109:524-31.
34. Caron G, Delneste Y, Roelandts E, Duez C, Bonnefoy JY, Pestel J, et al. Histamine polarizes human dendritic cells into th2 cell-promoting effector dendritic cells. J Immunol 2001;167:3682-6.
35. Hori Y, Nihei Y, Kurokawa Y, Kuramasu A, Makabe-Kobayashi Y, Terui T, et al. Accelerated clearance of Escherichia coli in experimental peritonitis of histamine-deficient mice. J Immunol 2002;169:1978-83.
36. Taubes G. The bacteria fight back. Science 2008;321:356-61.
37. Callewaert L, Michiels CW. Lysozymes in the animal kingdom. J Biosci 2010;35:127-60.
38. Sagel SD, Sontag MK, Accurso FJ. Relationship between antimicrobial proteins and airway inflammation and infection in cystic fibrosis. Pediatr Pulmonol 2009;44:402-9.
39. Koch AL. Bacterial wall as target for attack: Past, present, and future research. Clin Microbiol Rev 2003;16:673-87.
40. Kanthiah S, Kannappan V. Chemometric screening and optimization of liquid chromatographic method for simultaneous determination of seven antihistamines. Int J Pharm Pharm Sci 2016;8:288-95.
41. Aboelyazeed H, El-haggar S, Okasha K. Comparative study between the effect of histamine receptor antagonists of type II (Famotidine) and proton pump inhibitors (omeprazole) on the efficacy of calcium carbonate as phosphate binder in haemodialysis patient. Int J Pharm Pharm Sci 2017;9:10-4.
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How to Cite
PRITAM GOSWAMI, SWAIF ALI SK, SAYAK GHOSH, ANAMIKA BASU, JOYDEEP KHANRA, and SATADAL DAS. “EFFECT OF ULTRA-DILUTED HISTAMINE ON HYPOXIC CHICK LUNG TISSUE INFLAMMATORY CHANGES”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 7, May 2019, pp. 199-02, doi:10.22159/ajpcr.2019.v12i7.33651.
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