• METI WIDIYA LESTARI Department of Midwifes, Tasikmalaya Polytechnic of Health Ministry of Health, Tasikmalaya, Indonesia.
  • IRDA FIDRIANNY Pharmaceutical Biology Research Group, School of Pharmacy, Bandung Institute of Technology, Indonesia.
  • ANDREANUS A SOEMARDJI Pharmacology-Clinical Pharmacy Research Group, School of Pharmacy, Bandung Institute of Technology, Indonesia.



Fetal rat development, Teratogen, Liman leaves


Objective: The objective of the study was to observe the effect of liman leaves extract on fetal rat development and provide safety information for its use during pregnancy.

Methods: Estrus cycles of female Wistar rats were observed and mated to male rats, 0 day of pregnancy determined after finding a vaginal plug or in a vaginal smear, there was sperm. Sample was administered orally using sonde with a single administration on the 11th day of pregnancy, at doses of 3750 mg/kg bw, 1185 mg/kg bw, and 375 mg/kg bw. On the 19th day of pregnancy, the rats were sacrificed and then observed the number of implantation, corpus luteum, intrauterine death, and fetal abnormalities.

Results: The dose of 375 mg/kg bw exposed the highest average implantation rate (13.20%) and the largest number of corpus luteum (13.90%). The highest total intrauterine death was presented by dose of 3750 mg/kg bw and significantly different (p<0.05) compared to the control group. The dose of 375 mg/kg bw expressed the highest percentage of embryos with resorption (19.03%), while the lowest average fetal body was shown by 3750 mg/kg bw compared to the other groups. The highest percentage of external abnormalities was given by the dose of 375 mg/kg bw (12.91%), which abnormalities found were dwarf, cleft palate, hydrocephalus, short sleeve, and hematoma.

Conclusion: Liman leaves extract was mild teratogenic on Wistar rat fetus.


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How to Cite

METI WIDIYA LESTARI, IRDA FIDRIANNY, and ANDREANUS A SOEMARDJI. “THE EFFECT OF LIMAN LEAVES EXTRACT ON FETAL RAT DEVELOPMENT”. Asian Journal of Pharmaceutical and Clinical Research, vol. 12, no. 12, Dec. 2019, pp. 77-80, doi:10.22159/ajpcr.2019.v12i12.33780.



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