THE EFFECT OF LIMAN LEAVES EXTRACT ON FETAL RAT DEVELOPMENT
Objective: To observe the effect of liman leaves extract on fetal rat development and provide safety information for its use during pregnancy.
Methods: Oestrus cycles of female Wistar rats was observed and mated to male rats, zero day of pregnancy determined after finding a vaginal plug or in a vaginal smear there was sperm. Sample was administered orally using sonde with a single administration on the 11th day of pregnancy, at doses of 3750 mg/kg bw, 1185 mg/kg bw and 375 mg/kg bw. On the 19th day of pregnancy,the rats were sacrificed and then observed the number of implantation, corpus luteum, intra uterine death, and fetal abnormalities.
Results: The dose of 375 mg/kg bwexposed the highest average implantation rate (13.20%) and the largest number of corpus luteum (13.90%). The highest total intrauterine death was presented by dose of 3750 mg/kg bw and significantly different (p <0.05) compared to the control group. The dose of 375 mg/kg bw expressed the highest percentage of embryos with resorption (19.03%), whilethe lowest average fetal body was shown by 3750 mg/kg bw compared to the other groups.The highest percentage of external abnormalities was given by dose of 375 mg/kg bw (12.91%), which abnormalities foundwere dwarf, cleft palate, hydrocephalus, short sleeve and haematoma.
Conclusion: Liman leaves extract was mild teratogenic on Wistar rat fetus.
Keywords:Fetal Rat Development,Teratogen,Liman leaves
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