Medicine COMPARATIVE STUDY OF WOUND HEALING ACTIVITY OF INDIAN MEDICINAL PLANT AND HERB AGAINST DIABETIC FOOT ULCER (PIPER NIGRUM AND ARACHIS HYPOGAEA)

  • KUMAR CHANDAN Department of Biotechnology, Dr. M.G.R. Educational and Research Institute, University Maduravoyal, Chennai, Tamil Nadu, India.
  • SHARMILA KJ Department of Biotechnology, Dr. M.G.R. Educational and Research Institute, University Maduravoyal, Chennai, Tamil Nadu, India.

Abstract

Objective: Diabetic foot ulcers (DFUs) are the most common cause of non-traumatic lower extremity amputations in developing countries. The aim of this pilot study was to evaluate the medicinal plant (fruits) and herbs (seeds) to meet their primary health-care needs healing to DFUs.


Methods: Three solvents were used to acquire extracts from powdered parts of the species. The extracts were used for phytochemical screening like a standard procedure. Quantitative assay of antioxidant activity of the extracts using free radical scavenging phenomena such as a 1,1-diphenyl-2- picrylhydrazyl-assay. The antimicrobial activity of the test organisms to the 11 plant extracts was screened using the agar well diffusion method (Perez et al., 1990). Hypotonicity-induced human red blood cell (HRBC) assay performs to determine anti-inflammatory activities.


Results: The presence of maximum antioxidant activity found in Arachis hypogaea (ethyl acetate) extract followed by Piper nigrum. The comparative study of these plants and herb species (P. nigrum and A. hypogaea), where P. nigrum, contains more total tannin contents and antioxidant compounds as persistent manner compares to A. hypogaea. Ethanolic and ethyl acetate, extracts of P. nigrum were studied for the in vitro antimicrobial and anti-inflammation against DFU contamination and HRBCs. P. nigrum treated on DFU contamination and HRBC, shown with maximum inhabitation properties for DFU (800 μg/mL).


Conclusions: The finding of the present investigation demonstrated that P. nigrum significantly more suppresses the growth of DFU contamination and induces anti-inflammatory activities follow by A. hypogaea.

Keywords: antioxidant, diabetic foot ulcer, Anti-Inflammation

References

1. Karri VV. Current perspective in the management of diabetic foot ulcers an overview on the Indian scenario. Int J Pharm Pharm Sci 2014;6:1-2.
2. Mariam TG, Alemayehu A, Tesfaye E, Mequannt W, Temesgen K, Yetwale F, et al. Prevalence of diabetic foot ulcer and associated factors among adult diabetic patients who attend the diabetic follow-up clinic at the university of gondar referral hospital, North West Ethiopia, 2016: Institutional-based cross-sectional study. J Diabetes Res 2017;2017:2879249.
3. Banu A, Noorul Hassan MM, Rajkumar J, Srinivasa S. Spectrum of bacteria associated with diabetic foot ulcer and biofilm formation: A prospective study. Australas Med J 2015;8:280-5.
4. Gupta D, Girija K. Evaluation of in vitro antioxidant and anti microbial activities of various spices of Indian origin. Int J Pharm Pharm Sci 2015;7:137-41.
5. Damanhouri ZA, Ahmad A. A review on therapeutic potential of Piper nigrum L. (Black Pepper): The king of spices. Med Aromat Plants 2014;3:161.
6. Arya SS, Salve AR, Chauhan S. Peanuts as functional food: A review. J Food Sci Technol 2016;53:31-41.
7. Kumar SR, Sridhar C, Jayaveera KN. A study on total phenols, flavonoids content and antioxidant activity for the leaf extracts of Holostemma ada kodien schult traditionally used folklore medicinal plants. Indo Am J Pharm Res 2013;4:1499-512.
8. Akiyama H, Fujii K, Yamasaki O, Oono T, Iwatsuki K. Antibacterial action of several tannins against Staphylococcus aureus. J Antimicrob Chemother 2001;48:487-91.
9. Kumari M, Jain S. Tannin: An antinutrient with positive effect to manage diabetes. Res J Recent Sci 2012;1:1-8.
10. Dimitrios B. Sources of Natural Phenolic Antioxidants. Laboratory of Food Chemistry and Technology, School of Chemistry, Aristotle University. Thessaloniki: Elsevier Ltd.; 2006.
11. Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: A review. J Pharm Anal 2016;6:71-9.
12. Leelaprakash G, Dass SM. In vitro anti-inflammatory activity of methanol extract of Enicostemma axillare. Int J Drug Dev Res 2011;3:189-96.
13. Chen L, Deng H, Cui H, Fang J, Zuo Z, Deng J, et al. Inflammatory responses and inflammation-associated diseases in organs. Oncotarget 2018;9:7204-18.
14. Slinkard K, Singleton VL. Total phenol analysis: Automation and comparison with manual methods. Am J Enol Viticult 1997;28:49-55.
15. Somani Z, Bhattacharjee R, Chodankar M, Abhilash N. In vitro antioxidant, anti-inflammatory, inovo anti-angiogenic activities virtual screening of phyto constituents of chromolenaodorata. Int J Curr Res 2014;6:7766-71.
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KUMAR CHANDAN, and SHARMILA KJ. “Medicine COMPARATIVE STUDY OF WOUND HEALING ACTIVITY OF INDIAN MEDICINAL PLANT AND HERB AGAINST DIABETIC FOOT ULCER (PIPER NIGRUM AND ARACHIS HYPOGAEA)”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 11, Sept. 2019, pp. 35-39, doi:10.22159/ajpcr.2019.v12i11.33853.
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