• NELISIWE PRENATE MASUKU Department of Life and Consumer Sciences, University of South Africa, Cnr Christiaan de Wet and Pioneer Ave, Private Bag X6, Florida, 1710, South Africa.
  • SOGOLO LUCKY LEBELO Department of Life and Consumer Sciences, University of South Africa, Cnr Christiaan de Wet and Pioneer Ave, Private Bag X6, Florida, 1710, South Africa.


Objective: The study reports the effects of acetone, methanol, and aqueous Kigelia africana extracts on the TM3 Leydig cells.

Methods: The total phenolic contents were determined using Folin–Ciocalteu’s method. Antioxidant activity was determined using 1,1-diphenyl-2- picrylhydrazyl assay and flavonoid content assessed using quercetin as standard. Cell viability and cytotoxicity were assessed.

Results: Total phenolic and flavonoid contents of the extracts were analyzed. Acetone extracts showed highest total phenolic content (105±3.65 mg gallic acid equivalent [GAE]/g) followed by methanol extracts (10.64±0.32 mg GAE/g) and, last, the water extract with the total phenolic content of 4.27±0.65 mg GAE/g. The same trend was observed with total flavonoid content where results recorded for acetone, methanol, and water extracts were 3.63 ±0.20 mg/g quercetin equivalent (QE), 0.16±0.01 mg QE/g, and 0.01±0.00 mg QE/g, respectively. The three extracts showed lower IC50 values compared to ascorbic acid (0.143±0.05 mg/ml, 0.023±0.004 mg/l, and 0.043±0.03 mg/ml for acetone, methanol, and water extracts, respectively). At 0.5 mg/ml of acetone extract, there was an increase in the testosterone production compared to the control.

Conclusion: The findings of this study showed that K. africana could be recommended for use in the treatment of male infertility.

Keywords: Kigelia africana (Lam.) Benth., Antioxidants, Cytotoxicity, TM3 Leydig cells testosterone


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How to Cite
NELISIWE PRENATE MASUKU, and SOGOLO LUCKY LEBELO. “INVESTIGATION OF THE EFFECTS OF KIGELIA AFRICANA (LAM.) BENTH. EXTRACTS ON TM3 LEYDIG CELLS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 10, July 2019, pp. 87-92,
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