• SREEKALA KANNIKULATHEL GOPIDAS Centre for Advanced Studies in Botany, University of Madras, Chennai, Tamil Nadu, India.
  • NAGARAJ SUBRAMANI Centre for Advanced Studies in Botany, University of Madras, Chennai, Tamil Nadu, India.


Objective: In the present study, fucoidan extracted from three brown algae, Sargassum wightii, Turbinaria ornata, and Padina tetrastromatica, was purified, characterized, and evaluated for antioxidant and cytotoxic properties.

Methods: Algal powders were sequentially extracted with five solvents based on polarity and residue was subjected to acidic extraction. The filtrates were precipitated for alginates, and resultant supernatant was precipitated for fucoidan. The precipitate was centrifuged; pellet dialyzed and lyophilized to yield crude fucoidan, which was purified by diethylaminoethyl cellulose chromatography and characterized by biochemical tests and Fourier-transform infrared (FT-IR) spectrometry. Solvent extracts and fucoidans were subjected to 2,2-diphenyl-1-picrylhydrazyl assay. Fucoidans were subjected to trypan blue cytotoxicity assay.

Results: Antioxidant activity was highest in methanol extracts and Padina crude fucoidan, while lowest in hexane extracts and purified Sargassum fucoidan. Sargassum yielded the highest amount of fucoidan (7.14%). Total carbohydrates increased as Sargassum> Padina > Turbinaria, sulfates as Padina > Turbinaria > Sargassum, and protein content was 0.16±0.001%. Cytotoxicity increased in a dose-dependent manner; the highest and lowest for Padina at 200 mg mL-1 (40%) and 10 mg mL-1 (4%), respectively. Antioxidant and cytotoxic properties exhibited a positive correlation with sulfate content. FT-IR spectral values were characteristic to fucoidan.

Conclusion: Fucoidans from the three algae effectively scavenged free radicals and showed good cytotoxic activity. There was a positive correlation between sulfate content and bioactivity of fucoidans, supporting its structure-function relationship. Thus, extracts and fucoidans from these algae are found to be potential candidates for pharmacological applications.

Keywords: Antioxidant, Cytotoxic, Fucoidan, Brown algae, Sargassum wightii, Turbinaria ornata, Padina tetrastromatica

Author Biography

NAGARAJ SUBRAMANI, Centre for Advanced Studies in Botany, University of Madras, Chennai, Tamil Nadu, India.

Dr. Nagaraj Subramani is Assitant Professor at the Centre for Advanced Studies in Botany, University of Madras, Guindy Campous, Chennai, Tamil Nadu, India.


1. Wang ZJ, Zheng L, Yang JM, Kang Y, Park YD. Proteomic analyses for profiling regulated proteins/enzymes by Fucus vesiculosus fucoidan in B16 melanoma cells: A combination of enzyme kinetics functional study. Int J Biol Macromol 2018;112:667-74.
2. Balboa EM, Conde E, Moure A, Falqué E, Domínguez H. In vitro antioxidant properties of crude extracts and compounds from brown algae. Food Chem 2013;138 Suppl 2-3:1764-85.
3. Deniaud-Bouët E, Hardouin K, Potin P, Kloareg B, Hervé C. A review about brown algal cell walls and fucose-containing sulfated polysaccharides: Cell wall context, biomedical properties and key research challenges. Carbohydr Polym 2017;175:395-408.
4. Sanjeewa KKA, Lee JS, Kim WS, Jeon YJ. The potential of brown-algae polysaccharides for the development of anticancer agents: An update on anticancer effects reported for fucoidan and laminaran. Carbohydr Polym 2017;177:451-9.
5. Wu L, Sun J, Su X, Yu Q, Yu Q, Zhang P, et al. A review about the development of fucoidan in antitumor activity: Progress and challenges. Carbohydr Polym 2016;154:96-111.
6. Omar HE, Eldien HM, Badary MS, Al-Khatib BY, Abd Elgaffar SK. The immunomodulating and antioxidant activity of fucoidan on the splenic tissue of rats treated with cyclosporine. A J Basic Appl Zool 2013;66 Suppl 5:243-54.
7. Ngo DH, Kim SK. Sulfated polysaccharides as bioactive agents from marine algae. Int J Biol Macromol 2013;62:70-5.
8. Dore CM, Alves MG, Will LS, Costa TG, Sabry DA, De Souza Rêgo LA, et al. A sulfated polysaccharide, fucans, isolated from brown algae Sargassum vulgare with anticoagulant, antithrombotic, antioxidant and anti-inflammatory effects. Carbohydr Polym 2013;91 Suppl 1:467-75.
9. Chen A, Lan Y, Liu J, Zhang F, Zhang L, Li B, et al. The structure property and endothelial protective activity of fucoidan from Laminaria japonica. Int J Biol Macromol 2017;105:1421-9.
10. Synytsya A, Bleha R, Synytsya A, Pohl R, Hayashi K, Yoshinaga K, et al. Mekabu fucoidan: Structural complexity and defensive effects against avian influenza A viruses. Carbohydr Polym 2014;111:633-44.
11. Phull AR, Majid M, Haq IU, Khan MR, Kim SJ. In vitro and in vivo evaluation of anti-arthritic, antioxidant efficacy of fucoidan from Undaria pinnatifida (Harvey) Suringar. Int J Biol Macromol 2017;97:468-80.
12. Phull AR, Kim SJ. Fucoidan as bio-functional molecule: Insights into the anti-inflammatory potential and associated molecular mechanisms. J Funct Foods 2017;38:415-26.
13. Sun Y, Hou S, Song S, Zhang B, Ai C, Chen X, et al. Impact of acidic, water and alkaline extraction on structural features, antioxidant activities of Laminaria japonica polysaccharides. Int J Biol Macromol 2018;112:985-95.
14. Yuan Y, Macquarrie D. Microwave assisted extraction of sulfated polysaccharides (fucoidan) from Ascophyllum nodosum and its antioxidant activity. Carbohydr Polym 2015;129:101-7.
15. Kadam SU, Tiwari BK, Smyth TJ, O’Donnell CP. Optimization of ultrasound assisted extraction of bioactive components from brown seaweed Ascophyllum nodosum using response surface methodology. Ultrason Sonochem 2015;23:308-16.
16. Nagao T, Kumabe A, Komatsu F, Yagi H, Suzuki H, Ohshiro T. Gene identification and characterization of fucoidan deacetylase for potential application to fucoidan degradation and diversification. J Biosci Bioeng 2017;124 Suppl 3:277-82.
17. Ale MT, Meyer AS. Fucoidans from brown seaweeds: An update on structures, extraction techniques and use of enzymes as tools for structural elucidation. RSC Adv 2013;3 Suppl 22:8131-41.
18. Hifney AF, Fawzy MA, Abdel-Gawad KM, Gomaa M. Industrial optimization of fucoidan extraction from Sargassum sp. and its potential antioxidant and emulsifying activities. Food Hydrocoll 2016;54:77-88.
19. Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, et al. Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer 2015;136 Suppl 5:E359-86.
20. Gutiérrez-Rodríguez AG, Juárez-Portilla C, Olivares-Bañuelos T, Zepeda RC. Anticancer activity of seaweeds. Drug Discov Today 2017;23 Suppl 2:434-47.
21. Suresh V, Senthilkumar N, Thangam R, Rajkumar M, Anbazhagan C, Rengasamy R, et al. Separation, purification and preliminary characterization of sulfated polysaccharides from Sargassum plagiophyllum and its in vitro anticancer and antioxidant activity. Process Biochem 2013;48 Suppl 2:364-73.
22. Dubois M, Gilles KA, Hamilton JK, Rebers PT, Smith F. Colorimetric method for determination of sugars and related substances. Anal Chem 1956;28 Suppl 3:350-6.
23. Dodgson K, Price R. A note on the determination of the ester sulphate content of sulphated polysaccharides Biochem J 1962;84 Suppl 1:106.
24. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72 Suppl 1-2:248-54.
25. Dische Z, Shettles LB. A specific color reaction of methylpentoses and a spectrophotometric micromethod for their determination. J Biol Chem 1948;175 Suppl 2:595-603.
26. Vani T, Rajani M, Sarkar S, Shishoo C. Antioxidant properties of the ayurvedic formulation Triphala and its constituents. Int J Pharmacogn 1997;35 Suppl 5:313-7.
27. Eluvakkal T, Shanthi N, Murugan M, Arunkumar K. Extraction of antibacterial substances, galactofucoidan and alginate successively from the Gulf of Mannar brown seaweed Sargassum wightii Greville ex J. Agardh. Indian J Nat Prod Res 2014;5 Suppl 3:249-57.
28. Marudhupandi T, Kumar TT, Lakshmanasenthil S, Suja G, Vinothkumar T. In vitro anticancer activity of fucoidan from Turbinaria conoides against A549 cell lines. Int J Biol Macromol 2015;72:919-23.
29. Sinurat E, Rosmawaty P, Saepudin E. Characterization of fucoidan extracted from binuangeun’s brown seaweeds. Int J Chem Environ Biol Sci 2015;3:329-32.
30. Delma CR, Somasundaram ST, Srinivasan GP, Khursheed M, Bashyam MD, Aravindan N. Fucoidan from Turbinaria conoides: A multifaceted ‘deliverable’ to combat pancreatic cancer progression. Int J Biol Macromol 2015;74:447-57.
31. Thuy TT, Van TT, Hidekazu Y, Hiroshi U. Fucoidan from Vietnam Sargassum swartzii: Isolation, Characterization and Complexation with Bovine Serum Albumin. Asian J Chem 2012;24 Suppl 8:3367-70.
32. Vasquez RD, Ramos JD, Bernal SD. Chemopreventive properties of sulfated polysaccharide extracts from Sargassum siliquosum JG Agardh (Sargassaceae). Int J Phar Bio Sci 2012;3:333-45.
33. Wang CY, Chen YC. Extraction and characterization of fucoidan from six brown macroalgae. J Mar Sci Technol 2016;24 Suppl 2:319-28.
34. Yang WN, Chen PW, Huang CY. Compositional characteristics and in vitro evaluations of antioxidant and neuroprotective properties of crude extracts of fucoidan prepared from compressional puffing-pretreated Sargassum crassifolium. Mar Drugs 2017;15 Suppl 6:183.
35. Wang CY, Wu TC, Hsieh SL, Tsai YH, Yeh CW, Huang CY. Antioxidant activity and growth inhibition of human colon cancer cells by crude and purified fucoidan preparations extracted from Sargassum cristaefolium. J Food Drug Anal 2015;23 Suppl 4:766-77.
36. Cunha L, Grenha A. Sulfated seaweed polysaccharides as multifunctional materials in drug delivery applications. Mar Drugs 2016;14:e42.
37. Syad AN, Shunmugiah KP, Kasi PD. Antioxidant and anti-cholinesterase activity of Sargassum wightii. Pharm Biol 2013;51:1401-10.
38. Suresh V, Kumar NS, Murugan P, Palani P, Rengasamy R, Anbazhagan C. Antioxidant properties of sequential extracts from brown seaweed, Sargassum plagiophyllum, C. Agardh. Asian Pac J Trop Dis 2012;2:S937-9.
39. Anjana A, Ahamed KN, Ravichandiran V, Sumithra M, Anbu J. Anticancer activity of Sargassum wightii Greville on Dalton’s ascitic lymphoma. Chin J Nat Med 2014;12 Suppl 2:114-20.
40. Dhinakaran DI, Geetha P, Rajalakshmi J. Antioxidant activities of marine algae Valoniopsis pachynema and Sargassum swartzii from the Southeast coast of India. Int J Fish Aquat Stud 2015;3:426-30.
41. Palanisamy S, Vinosha M, Manikandakrishnan M, Anjali R, Rajasekar P, Marudhupandi T, et al. Investigation of antioxidant and anticancer potential of fucoidan from Sargassum polycystum. Int J Biol Macromol 2018;116:151-61.
42. Vaikundamoorthy R, Krishnamoorthy V, Vilwanathan R, Rajendran R. Structural characterization and anticancer activity (MCF7 and MDA-MB-231) of polysaccharides fractionated from brown seaweed Sargassum wightii. Int J Biol Macromol 2018;111:1229-37.
43. Usoltseva RV, Anastyuk SD, Ishina IA, Isakov VV, Zvyagintseva TN, Thinh PD, et al. Structural characteristics and anticancer activity in vitro of fucoidan from brown alga Padina boryana. Carbohydr Polym 2018;184:260-8.
44. Jose GM, Raghavankutty M, Kurup GM. Sulfated polysaccharides from Padina tetrastromatica induce apoptosis in hela cells through ROS triggered mitochondrial pathway. Process Biochem 2018;68:197-204.
45. Rajasekar T, Shamya AM, Joseph J. Screening of phytochemical, antioxidant activity and antibacterial activity of marine seaweeds. Int J Pharm Pharm Sci 2019;11 Suppl 1:61-6.
46. Dhanraj V, Manivasagam T, Karuppaiah J. Myricetin isolated from Turbinaria ornata ameliorates rotenone induced Parkinsonism in Drosophila melanogaster. Int J Pharm Pharm Sci 2017;9 Suppl 11:39-44.
47. Ashwini S, Babu TV, Saritha, Shantaram M. Seaweed extracts exhibit anticancer activity against HeLa cells lines. Int J Curr Pharm Res 2017;9 Suppl 1:114-7.
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How to Cite
SREEKALA KANNIKULATHEL GOPIDAS, and NAGARAJ SUBRAMANI. “IN VITRO ANTIOXIDANT AND CYTOTOXIC PROPERTIES OF FUCOIDAN FROM THREE INDIAN BROWN SEAWEEDS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 9, July 2019, pp. 99-105, doi:10.22159/ajpcr.2019.v12i9.34164.
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