COMPARATIVE PHYTOCHEMICAL PROFILE AND ANTIOXIDANT PROPERTY OF BARK, FLOWERS AND LEAVES EXTRACTS OF SIMAROUBA GLAUCA

  • SAJEEDA N Department of PG Studies and Research in Biochemistry, Tumkur University, Tumkur, Karnataka, India.
  • KOLGI RR Department of PG Studies and Research in Biochemistry, Tumkur University, Tumkur, Karnataka, India.
  • SHIVAKUMARA SL Department of PG Studies and Research in Biochemistry, Tumkur University, Tumkur, Karnataka, India.
  • SHIVARAJ Y Department of Chemistry and Biochemistry, Government Science College, Bengaluru, Karnataka, India.
  • KARIGAR CS Department of PG Studies and Research in Biochemistry, Bangalore University, Bengaluru, Karnataka, India.

Abstract

Objective: The current study was to evaluate and compare the phytochemical constituents and antioxidant activity of bark, flowers and leaves of the tree Simarouba glauca.


Methods: The solvent extraction of phytochemicals was carried out using Soxhlet apparatus with ethanol, chloroform, methanol, and water. The antioxidant property was determined by 2,2-Diphenyl-1-picrylhydrazyl, hydrogen peroxide free radical scavenging, reducing power assay, and nitric oxide radical scavenging assay using gallic acid and ascorbic acid as the standards.


Results: The extraction yield was found maximum in the water extract of flower (3.7% w/w). Qualitative and quantitative analysis of phytoconstituents showed that the highest amount of alkaloids and flavonoid content (2.1% w/w) and (3.9% w/w), respectively, was in the chloroform extract of the flower. Phenol and carbohydrate constituent was found to be highest in the methanol extract of leaves 2.5% w/w and 2.2% w/w, respectively. The antioxidant assays showed that the bark possessed maximum antioxidant activity. The water extracts of S. glauca bark exhibited scavenging property (90%) with an IC50 value 39.63 μg/ml, and the least activity (56%) was observed in the methanol extracts of leaves with an IC50 value of 62.96 μg/ml of S. glauca.


Conclusion: The study concluded that the water extract of the bark is a potent antioxidant compared to leaves and flowers. Further, in vivo studies are essential to enumerate its medicinal use and prove its efficacy in therapeutic applications.

Keywords: Simarouba glauca, Solvent extraction, Phytochemical constituents, Antioxidant activity, In vitro methods

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SAJEEDA N, KOLGI RR, SHIVAKUMARA SL, SHIVARAJ Y, and KARIGAR CS. “COMPARATIVE PHYTOCHEMICAL PROFILE AND ANTIOXIDANT PROPERTY OF BARK, FLOWERS AND LEAVES EXTRACTS OF SIMAROUBA GLAUCA”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 9, July 2019, pp. 56-63, https://innovareacademics.in/journals/index.php/ajpcr/article/view/34211.
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