TO STUDY THE ULCEROPROTECTIVE EFFECT OF LEAVES OF MORINGA OLEIFERA ON EXPERIMENTALLY INDUCED INFLAMMATORY BOWEL DISEASE ON ANIMAL MODELS

  • SHIPRA KAUSHIK Department of Pharmacology, GS Medical College and Hospital, Pilkhuwa, Hapur, Uttar Pradesh, India.
  • SHOBHIT KAUSHIK Department of Anaesthesiology, Assam Medical College and Hospital, Dibrugarh, Assam, India.

Abstract

Objective: The present study was carried out to evaluate the ulceroprotective effect of ethanolic extract of leaves of Moringa oleifera (EEMO) on experimentally induced inflammatory bowel disease (IBD) on Albino (Wistar strain) rats.


Methods: The rats were divided into four groups of six animals each. Group A and Group B received gum acacia per orally (P.O.), Group C received EEMO in dose of 500 mg/kg/day P.O., and Group D received 5-aminosalicylic acid (5-ASA) in the dose of 100 mg/kg/day P.O. All the animals were pre-treated with respective drugs (volume of drugs was kept constant at 10 ml/kg) for 5 days, along with the normal diet. On 5th day, animals were kept fasting for 12 h (overnight), and IBD was induced next morning in Groups B, C, and D by the administration of 1 ml of 4% acetic acid solution transrectally (TR). Group A (normal control) received 0.9% normal saline TR instead.


Results: The results have been shown that EEMO has significant activity against experimentally induced IBD when compared to the experimental control, with near normalization of colon architecture both macroscopically as well as microscopically. Tissue oxidative stress was reduced with significant improvement in tissue levels of superoxide dismutase (SOD) and catalase (CAT). Furthermore, significant improvement in levels of myeloperoxidase (MPO) was observed.


Conclusion: It is concluded that EEMO has got potent activity against experimentally induced IBD due to its anti-inflammatory and antioxidant properties.

Keywords: Anti-inflammatory, Moringa oleifera, Inflammatory bowel disease

References

1. Datt N, Patyar RR, Patyar S. Comparative evaluation of different doses of vinpocetine alone and in combination with sulfasalazine in experimentally induced inflammatory bowel disease in rats. Asian J Pharm Clin Res 2017;10:88-93.
2. Pengkumsri N, Suwannalert P, Sivamaruthi BS, Wongpoomchai R, Sirisattha S, Tammasakchai A, et al. Molecular, histological, and anti-oxidant evaluation of colitis induction in rats by different concentration of dextran sodium sulfate (5 kda). Int J Pharm Pharm Sci 2015;7:283-7. Available from: https://www.innovareacademics.in/journals/index.php/ijpps/article/view/9356.
3. Gottfried IS, Nash HR, Devlin EW. Presence of novel forms in the colon of inflammatory bowel disease patients. Am J Gastroenterol 1999;94:537-9.
4. Ko JK, Lam FY, Cheung AP. Amelioration of experimental colitis by Astragalus membranaceus through anti-oxidation and inhibition of adhesion molecule synthesis. World J Gastroenterol 2005;11:5787-94.
5. Simmonds NJ, Rampton DS. Inflammatory bowel disease a radical view. Gut 1993;34:865-8.
6. Ramakrishna BS, Varghese R, Jayakumar S, Mathan M, Balasubramanian KA. Circulating antioxidants in ulcerative colitis and their relationship to disease severity and activity. J Gastroenterol Hepatol 1997;12:490-4.
7. Sood A, Midha V, Sood N, Bhatia AS, Avasthi G. Incidence and prevalence of ulcerative colitis in Punjab, North India. Gut 2003;52:1587-90.
8. Mishra G, Singh P, Verma R, Kumar S, Srivastavi S, Jha K, et al. Traditional uses, phytochemistry and pharmacological properties of Moringa oleifera plant; an Overview. Der Pharm Lett 2011;3:141-64.
9. Fatima N, Fatima SJ. Pharmacological screening for anti-arthritic activity of Moringa oleifera. Asian J Pharm Clin Res 2016;9:106-11.
10. Remengton. Solution, Emulsion, Suspension and Extracts. The Science and Practice of Pharmacology. 19th ed. Pennsylvania: Mach Publishing Company; 1995. p. 1495-523.
11. CPCSEA (Committee for the Purpose of Control and Supervision on Experiments on Animals). CPCSEA guidelines for laboratory animal facility. Indian J Pharmacol 2003;35:257-74.
12. Zeytunlu M, Korkut M, Akgün E, Firat O, Aynaci M, Içöz G, et al. The comparative effects of calcium channel blockers in an experimental colitis model in rats. Turk J Gastroenterol 2004;15:243-9.
13. Morris GP, Beck PL, Herridge MS, Depew WT, Szewczuk MR, Wallace JL, et al. Hapten-induced model of chronic inflammation and ulceration in the rat colon. Gastroenterology 1989;96:795-803.
14. BEERS RF Jr., SIZER IW. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem 1952;195:133-40.
15. Krawisz JE, Sharon P, Stenson WF. Quantitative assay for acute intestinal inflammation based on myeloperoxidase activity. Assessment of inflammation in rat and hamster models. Gastroenterology 1984;87:1344-50.
16. OECD/OCDE. 425 Guideline. OECD Guidelines for Testing of Chemicals. Acute Oral Toxicity Up-and-Down-Procedure; 2005. p. 1-26.
17. Souad L, Dalila N, Cherifa A. Hyperhomocysteinemia lead to transmural inflammation of colon and increase severity of disease in acetic acid-induced colitis in rat. Int J Pharm Pharm Sci 2017;9:108-16.
18. Dutta S, Das S. Effects of the leaves of Moringa oleifera in experimentally induced colitis in animal models. IJGP 2011;5:55-60.
19. Kanodia L, Borgohain M, Das S. Effect of fruit extract of Fragaria vesca L. On experimentally induced inflammatory bowel disease in albino rats. Indian J Pharmacol 2011;43:18-21.
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SHIPRA KAUSHIK, and SHOBHIT KAUSHIK. “TO STUDY THE ULCEROPROTECTIVE EFFECT OF LEAVES OF MORINGA OLEIFERA ON EXPERIMENTALLY INDUCED INFLAMMATORY BOWEL DISEASE ON ANIMAL MODELS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 8, June 2019, pp. 103-6, https://innovareacademics.in/journals/index.php/ajpcr/article/view/34227.
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