PROTECTIVE EFFECT OF ROTENONE AGAINST LIPOPOLYSACCHARIDE AND D-GALACTOSAMINE-INDUCED HEMATOTOXICITY

Hematoprotective efficacy of Rotenone

  • SAMRAT RAKSHIT Department of Zoology, Toxicology and Pharmacology Laboratory, Guru Ghasidas University, Bilaspur, Chhattisgarh, India.
  • ANJANI VERMA Department of Zoology, Toxicology and Pharmacology Laboratory, Guru Ghasidas University, Bilaspur, Chhattisgarh, India.
  • SATENDRA KUMAR NIRALA Department of Rural Technology and Social Development, Laboratory of Natural Products, Guru Ghasidas University, Bilaspur, Chhattisgarh, India.
  • MONIKA BHADAURIA Department of Zoology, Toxicology and Pharmacology Laboratory, Guru Ghasidas University, Bilaspur, Chhattisgarh, India.

Abstract

Objective: The aim of the present study was to investigate the protective efficacy of rotenone against lipopolysaccharide (LPS) and D-galactosamine (D-GalN)-induced altered hematology.


Methods: Hematotoxicity was induced by coinjection of LPS (50 μg/kg i.p.) and D-GalN (300 mg/kg i.p.). Rotenone (5, 10 and 20 mg/kg p.o.) was administered for 6 days as a pre-treatment. Blood was collected through puncturing the retro-orbital sinus to analyze blood parameter. Serum was separated to analyze glucose, triglyceride, and cholesterol.


Results: The present study revealed decreased in red blood cells, platelets, hemoglobin, and hematocrit value while a significant increase in white blood cells, lymphocyte, and monocytes were observed in LPS and D-GalN treated rats. LPS and D-GalN administration significantly decrease glucose level while serum lipid profile (triglycerides and cholesterol level) were increased significantly at 5% level of significance. LPS and D-GalN-induced altered hematological and serological variables were restored toward control by rotenone pretreatment for 6 days in dose-dependent manner.


Conclusion: It can be said that LPS and D-GalN administration resulted in alteration of various hematological parameters and rotenone at 20 mg/kg dose restored significant alteration toward control due to the presence of antioxidant activity of rotenone.

Keywords: Rotenone, Hematology, Serum lipid, Lipopolysaccharide, D-galactosamine

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SAMRAT RAKSHIT, ANJANI VERMA, SATENDRA KUMAR NIRALA, and MONIKA BHADAURIA. “PROTECTIVE EFFECT OF ROTENONE AGAINST LIPOPOLYSACCHARIDE AND D-GALACTOSAMINE-INDUCED HEMATOTOXICITY”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 8, June 2019, pp. 164-7, https://innovareacademics.in/journals/index.php/ajpcr/article/view/34324.
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