A IMPACT OF HYGROPHILA AURICULATA-GREEN MOSQUITOCIDAL ACTIVITY AGAINST MALARIA VECTOR, ANOPHELES STEPHENSI (DIPTERA: CULICIDAE)

HYGROPHILA AURICULATA-GREEN MOSQUITOCIDAL ACTIVITY AGAINST MALARIAL VECTOR

  • BALU SUBASH Department of Zoology, Annamalai University, Chidambaram, Tamil Nadu, India.
  • PERIYASAMY VIJAYAN Department of Zoology, Annamalai University, Chidambaram, Tamil Nadu, India.

Abstract

Objective: Mosquitoes are insect vectors responsible for the transmission of parasitic and viral infections to millions of people worldwide, with substantial morbidity and mortality. Insecticides of botanical origin may serve as suitable alternative bio-control techniques in the future.


Methods: The egg hatchability, growth regulatory, longevity, fecundity, and larvicidal activity of crude methanol leaf extract of Hygrophila auriculata were assayed for their toxicity tested against malarial vector mosquito, Anopheles stephensi.


Results: The eggs, larvae, larvae/pupae, and cumulative mortality were observed hatching rates for 100 ppm, 0–18 h (18 h exposed) at 24, 48, and 72 h, respectively, and mortality was 33.4, 44.6, 17.9, and 95.9% methanolic extract treatment, with the lethal concentration 50 (LC50)/LC90 values were 35.420 28/75.600 ppm. Effect of methanolic extract was larval, pupal, and adult duration and water extracts of caused longest delayed development from 16.6 days larvae, 7.5 days for pupae and longevity of adult female greatly reduced from 25.9 days and fecundity also reduced from 68.0 at 150 ppm. The larvicidal activity of methanol extract was decreased at 48 h as for instars larvae 63.44/271.95 ppm (I), 57.55/272.48 ppm (II); 62.49/301.22 ppm (III); 67.69/330.48 ppm (IV), and 76.99/343.82 ppm (pupae), respectively.


Conclusion: These results suggest that the methanol leaf extract has the potential to be used as an ideal eco-friendly approach for the control of mosquito vector. Therefore, this study provides on the mosquito eggs, larvae, and pupae activities of these plant methanol extract against A. stephensi.

Keywords: Hygrophila auriculata, Anopheles stephensi ovicidal, Growth regulatory, Larvicidal activity

Author Biography

PERIYASAMY VIJAYAN, Department of Zoology, Annamalai University, Chidambaram, Tamil Nadu, India.

Department of Zoology, Faculty of Science, Annamalainagar

References

1. World Health Organization. Malaria Fact Sheet, No. 9. Geneva: World Health Organization Press; 2015.
2. World Health Organization. Working to Overcome the Global Impact of Neglected Tropical Diseases: First WHO Report on Neglected Tropical Diseases. Geneva: Department of Reproductive Health and Research, World Health Organization; 2010. p. 172.
3. Golding N, Wilson AL, Moyes CL, Cano J, Pigott DM, Velayudhan R, et al. Integrating vector control across diseases. BMC Med 2015;13:249.
4. World Health Organization. Malaria Fact Sheet, No. 94. Geneva: World Health Organization; 2014.
5. Rathy MC, Sajith U, Harilal CC. Larvicidal efficacy of medicinal plant extracts against the vector mosquito Aedes albopictus. Int J Mosq Res 2015;2:80-2.
6. World Health Organization. World Malaria Report. Geneva: World Health Organization; 2016.
7. World Health Organization. World Malaria Report. Geneva: World Health Organization; 2018.
8. Baranitharan M, Tamizhazhagan V, Kovendan K. Medicinal plants as potent power for malaria control: A review. Entomol Appl Sci Lett 2019;5:28-44.
9. Bhuktar AS. In: Mungikar Am, Bhuktar AS, editor. Plant Resources Development. Aurangabad: Sarswati Printing Press; 2000. p. 146-52.
10. Jain SK. Dictionary of Indian Folk Medicine and Ethnobotany. New Delhi: Deep Publications; 1991. p. 105-6.
11. Bairaj P, Nagarajan S. Apigenin 7-O-glucuronide from the flowers of Asteracantha longifolia Nees. Indian Drug 1982;19:150-2.
12. Parashar VV, Harikishan S. Investigation of Asteracantha longifolia Nees. Indian J Pharmacol 1965;27:109-13.
13. Misra TN, Singh RS, Pandey HS, Pandey BK. Constituents of Asteracantha longifolia Nees. Fitoterapia 2001;72:194-6.
14. Choudhary BK, Bandyopdhyay NG. Important of mineral content and medicinal properties of Moringa oleifera and Hygrophila auriculata. Sachitra Ayurveda 1980;50:543-9.
15. Quasim C, Dutta NL. Reported the prescence of stigmasterol in the root of Asteracantha longifolia Nees. J Indian Chem Soc 1967;44:82-3.
16. Govindachari TR, Nagarajan K, Pai BR. Isolation of lupeol from the root of Asteracantha longifolia Nees. J Ind Sci Res 1957;16:72-7.
17. Shanmugasundaram P, Venkatraman S. Anti-nociceptive activity of Hygrophila auriculata (schum) Heine. Afr J Tradit Complement Altern Med 2005;2:62-9.
18. Mazumdar UK, Gupta M, Maiti S, Mukherjee D. Antitumor activity of Hygrophila spinosa on Ehrlich ascites carcinoma and sarcoma-180 induced mice. Indian J Exp Biol 1997;35:473-7.
19. Boily Y, Van Puyvelde L. Screening of medicinal plants of Rwanda (Central Africa) for antimicrobial activity. J Ethnopharmacol 1986;16:1-3.
20. Kumar KC, Müller K. Medicinal plants from Nepal; II. Evaluation as inhibitors of lipid peroxidation in biological membranes. J Ethnopharmacol 1999;64:135-9.
21. Vishnu SN, Sunil K, Ramdas P. Acute toxicity study of Hygrophila auriculata L. leaves methanolic extract in albino rats. J Pharm Chem Biol Sci 2015;3:388-95.
22. Su T, Mulla MS. Ovicidal activity of neem products (azadirachtin) against culex tarsalis and Culex quinquefasciatus (Diptera: Culicidae). J Am Mosq Control Assoc 1998;14:204-9.
23. World Health Organization. Guidelines for Laboratory and Field Testing of Mosquito Larvicides. Communicable Disease Control, Prevention and Eradication, WHO Pesticide Evaluation Scheme. Geneva: World Health Organization; 2005.
24. Abbott WS. A method of computing the effectiveness of an insecticide. J Eco Entomol 1925;18:265-7.
25. Finney DJ. Probit Analysis: A Ststistical Treatment of the Sigmoid Response Curve. London: Cambridge University Press; 1971. p. 633.
26. Baranitharan M, Dhanasekaran S, Murugan K, Kovendan K, Gokulakrishnan J. Chemical composition and laboratory investigation of Melissa officinalis essential oil against human malarial vector mosquito, Anopheles stephensi L. (Diptera: Culicidae). J Coast Life Med 2016;4:969-73.
27. Kovendan K, Murugan K, Kumar KP, Panneerselvam C, Kumar PM, Amerasan D, et al. Mosquitocidal properties of Calotropis gigantea (Family: Asclepiadaceae) leaf extract and bacterial insecticide Bacillus thuringiensis against the mosquito vectors. Parasitol Res 2012;111:531-44.
28. Swati S, Mansi P. Estimation of phytochemical components from Cassia tora and to study its larvicidal activity. Int J Pharm Sci Invent 1988;4:11-6.
29. Schmutterer H. Potential of Azdirachtin-containing pesticides for integrated pest control in developing and industrialized countries. J Insect Physiol 1988;34:713-9.
30. Dwijendra S, Sucheta SM, Nirmal KN, Yogendra NS, Mamta M. Now possible insect growth regulators from Catharanthus roseu. Cuur Sci 2003;84:1184-6.
31. Saxena SC, Yadav RS. A new plant extract to suppress the population of yellow fever and dengue vector Aedes aegypti (Diptera: Culicidae) Curr Sci 1983;52:713-5.
32. Saxena SC, Sumitra L. Laboratory evaluation of leaf extracts of new plant to suppress the population of malaria vector Anopheles stephnensi Liston (Diptera: Culicidae). J Am Mosq Control Assoc 1985;9:84-7.
33. Amalraj D, Vasugi V, Kalayanasundram M, Tay BK, Das PK. Laboratory and filed evaluation of three insect growth regulators against mosquito vectors. J Med Res 1988;87:24-31.
34. Thishimenan S, Baskaran J, Baranitharan M, Jeyasankar A. Laboratory investigation of Terminalia arjuna and Trachyspermum roxburghianum against groundnut pest, Helicoverpa armigera. Asian J Pharm Clin Res 2016;9:232-6.
35. Latif MS, Abbas S, Kormin F, Mustafa MK. Green synthesis of plant-mediated metal nanoparticles the role of polyphenols. Asian J Pharm Clin Res 2019;12:75-84.
36. Kovendan K, Chandramohan B, Dinesh D, Abirami D, Vijayan P, Vincent S, and Benelli G. Green-synthesized silver nanoparticles using Psychotria nilgiriensis: toxicity against the dengue vector Aedes aegypti (Diptera: Culicidae) and impact on the predatory efficiency of the non-target organism Poecilia sphenops (Cyprinodontiformes: Poeciliidae) J Asia Pacific Entomol 2016;19:1001-7.
37. Baranitharan M, Dhanasekaran S, Murugan K, Kovendan K, Gokulakrishnan J, Jeyasankar A. Experimental investigations of Nagapattinam indigenous medicinal plant extracts against dengue, malaria and filarial disease. Int J Zool Appl Biosci 2017;2:155-61.
38. Dhanasekaran S, Baranitharan M, Muthulingam M, Senthilmurugan S, Jeyasankar A, Sawicka B. Studies on the impact of medicinal plants in relation to malaria vector control against Anopheles stephensi. Innoriginal Int J Sci 2018;5:12-4.
39. Baranitharan M, Dhanasekaran S, Murugan K, Kovendan K, Gokulakrishnan J, Benelli G. Coleus aromaticus leaf extract fractions: A source ofnovel ovicides, larvicides and repellents against Anopheles, Aedes and Culex mosquito vectors? Proc Safe Environ Prot 2017;106:23-33.
40. Kovendan K, Murugan K, Vincent S. Mosquitocidal efficacy of Orthosiphon thymiflorus (Roth) sleesen. (Family: Labiatae) leaf extract and microbial insecticide, Metarhizium anisopliae against dengue vector, Aedes aegypti (Diptera: Culicidae). Divers Physiol Proc 2012;26:137-51.
41. Baranitharan M, Sawicka B, Gokulakrishnan J. Phytochemical profiling and larval control of Erythrina variegata methanol fraction against malarial and filarial vector. Adv Prev Med 2019;2019:2641959.
42. Kumar PM, Kovendan K, Murugan K. Integration of botanical and bacterial insecticide against Aedes aegypti and Anopheles stephensi. Parasitol Res 2013;112:761-71.
43. Oliveira-Filho EC, Paumgartten FJ. Toxicity of Euphorbia milii latex and niclosamide to snails and nontarget aquatic species. Ecotoxicol Environ Saf 2000;46:342-50.
44. Kovendan K, Murugan K, Vincent S. Evaluation of larvicidal activity of Acalypha alnifolia Klein ex Willd. (Euphorbiaceae) leaf extract against the malarial vector, Anopheles stephensi, dengue vector, Aedes aegypti and Bancroftian filariasis vector, Culex quinquefasciatus (Diptera: Culicidae). Parasitol Res 2012;110:571-81.
45. Murugan K, Kumar PM, Kovendan K, Amerasan D, Subrmaniam J, Hwang JS, et al. Larvicidal, pupicidal, repellent and adulticidal activity of Citrus sinensis orange peel extract against Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae). Parasitol Res 2012;111:1757-69.
46. Nathan SS, Kalaivani K, Murugan K. Effects of neem limonoids on the malaria vector Anopheles stephensi Liston (Diptera: Culicidae). Acta Trop 2005;96:47-55.
47. Senthilkumar N, Varma P, Gurusubramanian G. Larvicidal and adulticidal activities of some medicinal plants against the malarial vector, Anopheles stephensi (Liston). Parasitol Res 2009;104:237-44.
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BALU SUBASH, and PERIYASAMY VIJAYAN. “A IMPACT OF HYGROPHILA AURICULATA-GREEN MOSQUITOCIDAL ACTIVITY AGAINST MALARIA VECTOR, ANOPHELES STEPHENSI (DIPTERA: CULICIDAE)”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 9, Aug. 2019, pp. 306-10, https://innovareacademics.in/journals/index.php/ajpcr/article/view/34356.
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