• KALAIMAGAL C Department of Biotechnology, Marudupandiyar College of Arts and Science, Thanjavur, Tamil Nadu, India.


Objective: Herbs are a key resource with therapeutic properties. Nowadays, there is a focus on the identification of bioactive compounds with the ability to act against various disorders.

Methods: In the present study, gas chromatography–mass spectrometry analysis was conducted to determine the occurrence of different phytochemical compounds in ethanolic flower extract of Tabernaemontana divaricata (L.).

Results: The ethanol extract of flower revealed the presence of several bioactive compounds such as n-hexadecanoic acid, squalene, Vitamin D3, Vitamin A aldehyde, desulfosinigrin, and Urs-12-en-24-oic acid, 3-oxo-, methyl ester, (+)-.

Conclusion: The perceived compounds from ethanolic extract of flower have diverse beneficial properties such as antimicrobial, antioxidant, cancer anticipatory effect, pesticide, and antiarthritic.

Keywords: Tabernaemontana divaricata (L.), Gas chromatography–mass spectrometry analysis, Bioactive compounds


1. Asha KR, Priyanga S, Hemmalakshmi S, Devaki K. GC-MS analysis of the ethanolic extract of the whole plant Drosera indica L. J Pharmacogn Phytochem Res 2017;9:685-8.
2. Banakar P, Jayaraj M. GC-MS analysis of bioactive compounds from ethanolic leaf extract of Waltheria indica Linn. And their pharmacological activities. Int J Pharm Sci Res 2018;9:2005-10.
3. Mtunzi FM, Ejidike IP, Matamela T, Dikio E, Klink MJ. Phytochemical profiling, antioxidant and antibacterial activities of leaf extracts from Rhus leptodictya. Int J Pharmacogn Phytochem Res 2017;9:1090-9.
4. Sivakrishnan S, Pradeepraj D. Gas chromatography mass spectrocopy analysis of ethanolic extract of leaves of Cordia oblique Willd. Asian J Pharm Clin Res 2019;12:110-2.
5. Shunmugapriya K, Kalavathy U. GC-MS analysis of bioactive constituents of Rauwolfia densiflora (Wall) Benth. Ex Hk. F. Int J Appl Biol Pharm Technol 2012;3:179-83.
6. Kumar S, Samydurai P, Ramakrishnan R, Nagarajan N. Gas chromatography and mass spectrometry analysis of bioactive constituents of Adiantum capillus-veneris L. Int J Pharm Pharm Sci 2014;6:60-3.
7. Kalva S, Raghunandan N. Preliminary phytochemical screening and antimicrobial activity of dried flowers of Adenium obesum. Int J Curr Pharm Res 2019;11:34-6.
8. Dhivya R, Manimegalai K. Preliminary phytochemical screening and GC-MS profiling of ethanolic flower extract of Calotropis gigantea Linn. (Apocynaceae). J Pharmacogn Phytochem 2013;2:28-32.
9. Zaidan MR, Noor Rain A, Badrul AR, Adlin A, Norazah A, Zakiah I, et al. In vitro screening of five local medicinal plants for antibacterial activity using disc diffusion method. Trop Biomed 2005;22:165-70.
10. Nishaa S, Vishnupriya M, Sasikumar JM, Gopalakrishnan VK. Phytochemical screening and GC-MS analysis of ethanolic extract of rhizomes of Maranta arundinacea L. Res J Pharm Biol Chem Sci 2013;4:52-9.
11. Ashikur MD, Rahman MD. Evauation of anibacterial activity of study of leaves of Tabernaemontana divaricata (L). Int Res J Pharm 2011;2:123-7.
12. Rumzhum NN, Rahman MM, Kazal MK. Antioxidant and cytotoxic potential of methanol extract of Tabernaemontana divaricata leaves. Int Curr Pharm J 2012;1:27-31.
13. Rahman MM, Sayeed MA, Biplab KP, Siddique SA. Antidiabetic and cytotoxic activities of methanolic extract of Tabernaemontana divaricata (L.) leaves in alloxan induced mice. Asian J Pharm Clin Res 2012;5:49-52.
14. Xie Z, Liu Q, Liang Z, Zhao M, Yu X, Yang D, et al. The GC/MS analysis of volatile components extracted by different methods from exocarpium citri grandis. J Anal Methods Chem 2013;2013:1-8.
15. Hassanpouraghdam MB. Flowerhead volatile oil composition of soilless culture-grown Chrysanthemum balsamita L. Nat Prod Res 2009;23:672-7.
16. Arirudran B, Oswin MA, Balabhaskar R. GC/MS determination of bioactive components from Cuminum cyminum L. World J Pharm Res 2018;7:1671-83.
17. Warrier PK. Indian Medicinal Plants: A Compendium of 500 Species. Vol. 5. New York: Orient Longman Pvt., Ltd,; 1996. p. 232-4.
18. Kam TS, Loh KY, Wei C. Conophylline and conophyllidine: New dimeric alkaloids from Tabernaemontana divaricata. J Nat Prod 1993;56:1865-71.
19. Kam TS, Pang HS, Lim TM. Biologically active indole and bisindole alkaloids from Tabernaemontana divaricata. Org Biomol Chem 2003;1:1292-7.
20. Paranthaman R, Kumar PP, Kumaravel S. GC-MS Analysis of phytochemicals and simultaneous determination of flavonoids in Amaranthus caudatus (Sirukeerai) by RP-HPLC. J Anal Bioanal Tech 2012;3:147.
21. Kumaravel S, Kumar PP, Vasuki P. GC-MS Study on Microbial degradation of Lindane. Int J Appl Chem 2010;6:363-6.
22. Coruh N, Celep AS, Ozgokce F. Antioxidant properties of Prangos ferulacea (L.) Lindl., Chaerophyllum macropodum Boiss. and Heracleum persicum Desf. from Apiaceae family used as food in Eastern Anatolia and their inhibitory effects on glutathione-S-transferase. Food Chem 2007;100:1237-42.
23. Dasgupta N, De B. Antioxidant activity of Piper betle L. leaf extract in vitro. Food Chem 2004;88:219-24.
24. Buonfiglio LG, Cano M, Pezzulo AA, Vanegas Calderon OG, Zabner J, Gerke AK, et al. Effect of Vitamin D3 on the antimicrobial activity of human airway surface liquid: Preliminary results of a randomised placebo-controlled double-blind trial. BMJ Open Respir Res 2017;4:e211.
25. Gil Á, Plaza-Diaz J, Mesa MD. Vitamin D: Classic and novel actions. Ann Nutr Metab 2018;72:87-95.
26. Jeon SM, Shin EA. Exploring Vitamin D metabolism and function in cancer. Exp Mol Med 2018;50:20.
27. Jeong HY, Park KM, Lee MJ, Yang DH, Kim SH, Lee SY, et al. Vitamin D and hypertension. Electrolyte Blood Press 2017;15:1-1.
28. Acharya A, Halemani SS. Role of Vitamin D in diabetes mellitus. Int J Pharm Sci Res 2016;7:1881-8.
29. Bennasir H, Sridhar S, Abdel-Razek TT. Vitamin A from physiology to disease prevention. Int J Pharm Sci Rev Res 2010;1:68-73.
30. Huang Z, Liu Y, Qi G, Brand D, Zheng SG. Role of Vitamin A in the immune system. J Clin Med 2018;7:e28.
31. Timoneda J, Rodríguez-Fernández L, Zaragozá R, Marín MP, Cabezuelo MT, Torres L, et al. Vitamin A deficiency and the lung. Nutrients 2018;10:e1132.
32. Mohammed GJ, Omran AM, Hussein HM. Antibacterial and phytochemical analysis of Piper nigrum using gas chromatography-mass spectrum and fourier-transform infrared spectroscopy. Int J Pharmacogn Phytochem Res 2016;8:977-96.
33. Li S, Chen G, Zhang C, Wu M, Wu S, Liu Q. Research progress of natural antioxidants in foods for the treatment of diseases. Food Sci Hum Wellness 2014;3:110-6.
34. Gopinath S, Sakthidevi G, Muthukumaraswamy S, Mohan VR. GC-MS analysis of bioactive constituents of Hypericum mysorense (Hypericaceae). J Curr Chem Pharm Sci 2013;3:6-15.
35. Hussein HM. Analysis of trace heavy metals and volatile chemical compounds of Lepidium sativum using atomic absorption spectroscopy, gas chromatography-mass spectrometric and fourier-transform infrared spectroscopy. Res J Pharm Biol Chem Sci 2016;7:2529-55.
36. Madhavan M. Phytochemical constituents of leaves of Spatholobus parviflorus a rare threatened climber of South India. Int J Pharmacogn Phytochem Res 2015;7:991-4.
37. Al-Rubaye AF, Kaizal AF, Hameed IH. Phytochemical screening of methanolic leaves extract of Malva sylvestris. Int J Pharmacogn Phytochem Res 2017;9:537-52.
38. Srivastava R, Mukerjee A, Verma A. GC-MS analysis of phytocomponents in, pet ether fraction of Wrightia tinctoria seed. Pharmacogn J 2015;7:249-53.
39. Egbung GE, Anosike C, Utu-Baku AB, Ogar I, Udo NN. Phytochemical evaluation and GC-MS analysis of Hyptis verticillata cultivated in Calabar Cross River State, Nigeria. Int J Biol Chem Sci 2017;11:2548-59.
40. Wagner KH, Elmadfa I. Biological relevance of terpenoids. Overview focusing on mono-, di- and tetraterpenes. Ann Nutr Metab 2003;47:95-106.
41. Sasikala K, Mohan SC. Total phenolic, flavanoid contents and GC-MS analysis of Canthium coromandelicum leaves extract. Int J Pharm Pharm Sci 2014;6:379-81.
42. Jothi RS, Uthayakumari F, Bharathy V. Phytochemical profile of leaf samples of subspecies of Senna italica Mill. Biosci Discov 2015;6:106-11.
43. Vinothkanna A, Manivannan P, Muralitharan G, Sekar S. In silico probing of anti-arthritic potential of traditionally fermented ayurvedic polyherbal product balarishta reveals lupeol and desulphosinigrin as efficient interacting components with urec. Int J Pharm Pharm Sci 2014;6:469-75.
44. Swamy NT, Rosaiah G, Babu K, Kumar KV. A study on phytochemical composition, GC-MS analysis and anti-microbial potential of methanolic leaf extract of Alstonia scholaris (L.) R. BR. Int J Pharm Sci Res 2019;10:747-55.
45. Papitha R, Ravi L, Selvaraj CI. Phytochemical studies and GC-MS analysis of Spermadictyon suaveolens Roxb. Int J Pharm Pharm Sci 2017;9:143-9.
46. Kalaivani CS, Sathish SS, Janakiraman N, Johnson M. GC-MS studies on Andrographis paniculata (Burm. f.) Wall. Ex Nees a medicinally important plant. Int J Med Arom Plants 2012;2:69-74.
229 Views | 127 Downloads
How to Cite
KALAIMAGAL C. “IDENTIFICATION OF BIOACTIVE COMPOUNDS IN FLOWER OF TABERNAEMONTANA DIVARICATA (L.) USING GAS CHROMATOGRAPHY–MASS SPECTROMETRY ANALYSIS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 9, July 2019, pp. 129-32, doi:10.22159/ajpcr.2019.v12i9.34559.
Original Article(s)