• LOKENDRA SINGH Research Analyst, Bilwal Medchem, and Research Laboratory (BMRL) Pvt. Ltd., Rajasthan, India.


Objective: Herbal drugs are more beneficial better than aspirin because this is an herb so no side effect this drug and it is easy to collect or use to as herbal drugs. Words are inadequate to describe the motivation for my work given to my beloved guide. I would like to add special thanks to my guide Gauravbilwal, for their guidance, support, and encouragement.

Purpose (Hypothesis): The main purpose of this article pays to attention for herbal drugs because they are naturally old effective drugs. As well as, Ayurveda treatment is very older effective technique.

Design/Method: Haffner gave to this technique of determining analgesic are around in 1929.

Procedure: This technique according to tail if clipped with any object and tightly or will be compression generation of pain in the tail as well as mice starting to bite that portion of its tail, and could evaluate and recorded the response how much it bites tail quickly or in potential.

  • Using this simple yet important marvel, we may apply the drug to be evaluated and record the response whether it bites tail quickly or in potential.

  • If given drugs have analgesic likely, then rat will not bite its tail so frequently.

  • Mice that do not show any response within 15 s will reject from the experiment.

Results: The found in analgesic activity of additional compounds test to significant on tail flick test than acetic acid-induced test and thus it appears that the test compounds inhibit predominantly the peripheral pain mechanism. The results of the study indicate that the extracts of polyherbal plants of analgesic activity by reducing the abdominal constriction significantly and may supposed to have a possible role in inhibition of cyclooxygenase in the prostaglandin pathways (p****<0.0001, ***0.0001, *0.05).

Conclusion: The present study showed the significant analgesic effect of both aqueous and alcoholic at 400 mg/kg doses in albino rats, we reported for the 1st time analgesic effect of different plants (Curcuma longa, Colchicaceae, Colocynthis, Withania somnifera, and Achyranthes aspera) in Haffner’s tail clip models. Aspirin has each uncoated effervescent tablet content are acetylsalicylic acid I.P. 325 mg. Finally summarized in this article represent a most effective results of herbal drugs equalized allopathic drugs without any other side effect. Hence, this is very usefully combination of Ayurveda drugs.

Keywords: Haffner’s Tail Clip method, Albino Wistar rats and plant’s extracts.


1. Kojima H, Yanai T, Toyota A. Essential oil constituent’s from Japanese and Indian curcuma aromatic rhizomes. Planta Med 1998;64:380-1.
2. Clap ham AR, Tutin TG, Warburg EF. Flora of the British Isles. Cambridge: Cambridge University Press; 1962. p. 982.
3. Botanical Society of Britain and Ireland on 2015-01-25. BSBI List; 2007. Available from: ???. [Last accessed on 2014 Nov 17].
4. Parnell J, Curtis T, Webb DA. Webb’s an Irish Flora. Cork: Cork University Press. 2012. p. 504.
5. ???. Monograph ‘Withaniasomnifera’. Altern Med Rev 2004;9:211-4.
6. Ven Murthy MR, Ranjekar PK, Ramassamy C, Deshpande M. Scientific basis for the use of Indian Ayurvedic medicinal plants in the treatment of neurodegenerative disorders: Ashwagandha. Cent Nerv Syst Agents Med Chem 2010;10:238-46.
7. Chandrasekhar K, Kapoor J, Anishetty S. A prospective, randomized double-blind, placebo-controlled study of safety and efficacy of a high-concentration full-spectrum extract of ashwagandha root in reducing stress and anxiety in adults. Indian J Psychol Med 2012;34:255-62.
8. Sharma CG. Ashwagandharishta Rastantra Sar Evam Sidhyaprayog Sangrah. Nagpur: Krishna-Gopal Ayurveda Bhawan (Dharmarth Trust); 1938. p. 743-4.
9. Arun V, Lieu VB, John JV, Reena B, Renuka PC. Traditional remedies of Kani tribes of Kottoor reserve forest, agasthyavanam, Thiruvananthapuram, Kerala. Indian J Tradit Knowl 2007;6:589-94.
10. Tijani Y, Uguru MO, Salawu OA. Anti-pyretic, anti-inflammatory and anti-diarrheal properties of Faidherbia albida in rats. Afr J Biotech 2008;7:696-700.
11. Pande PC, Tiwari L, Pande HC. Ethnoveterinary plants of uttaranchal a review. Indian J Tradit Knowl 2007;6:444-58.
12. Sachs CJ. Oral analgesics for acute nonspecific pain. Am Fam Physician 2005;71:913-8.
13. Derry CJ, Derry S, Moore RA. Caffeine as an analgesic adjuvant for acute pain in adults. Cochrane Database Syst Rev 2012;3:CD009281.
14. Satish AM, Brahadesh M, Kalabharathi HL, Pushpa VK, Jayanthi M, Suresh RN. Evaluation of analgesic activity of aqueous extract of fenugreek seeds in albino mice. Int J Recent Trends Sci Technol 2013;9:166-88.
15. Poonam SM, Jangde CR, Narnawal SD, Subhash R. Experimental evaluation of analgesic property of bark skin of Saraca indicia (Ashoka) and Shorea robusta (shal). J Appl Pharm Sci 2014;4:62-5.
16. Seema R, Mahesh CG. Evaluation and comparison of the antinociceptive activity of aspartame with sucrose. Pharm Rep 2012;64:293-8.
17. Meena MK, Jain AK, Gaur K, Kori ML, Take A, Nema RK. Screening of anti-inflammatory and analgesic activity of Cassia grandis Linn. Academic J Plant Sci 2009;2:51-5.
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How to Cite
LOKENDRA SINGH. “AN AN STUDY OF ANALGESIC ACTIVITY OF HAFFNER’S TAIL CLIP METHOD ON ALBINO WISTAR RATS OF SOME POLYHERBAL FORMULATION”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 9, July 2019, pp. 142-4, doi:10.22159/ajpcr.2019.v12i9.34561.
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