BIOACTIVE COMPOUND ANALYSIS AND ANTIOXIDANT ACTIVITY OF ENDOPHYTIC BACTERIAL EXTRACT FROM ADHATHODA BEDDOMEI

  • Swarnalatha Y sathyabama university
  • Bhaswati Saha sathyabama university
  • Lokeswara Choudary Nandanam arts college

Abstract

Objectives: The endophytic bacteria Lactobacillus sp. has been isolated from the tissues of plant leaves. The bacterial strains were grown in nutrient
broth at 37°C for 5 days. To isolate the bioactive compounds, the culture was grown in a broth and the culture broth was centrifuged and the
supernatant was collected.
Methods: Solvent-solvent method was applied to extract the bioactive compounds. The antioxidant activity was evaluated with the help of
1,1-diphenyl -2-picrylhydrazyl radical scavenging activity.
Results: The qualitative analysis of extracts indicated presence of carbohydrates, tannins, saponins, alkaloids, glycosides, proteins, amino acids, and
saponins. Flavonoids and anthocyanins were showed negative results, the quantitative analysis of the phenolic compounds showed the presence of
0.67 mg/ml. The inhibitory concentration 50 value for antioxidant activity was 35 μg/ml.
Conclusion: The antioxidant activity is due to the presence of different bioactive, and the high levels of phenolic compounds may be responsible for
antioxidant activity.

Keywords: Lactobacillus sp., Antioxidant activity, Endophytic bacteria, Phenolic compounds.

Author Biography

Swarnalatha Y, sathyabama university
Department of biotechnology

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How to Cite
Y, S., B. Saha, and L. Choudary. “BIOACTIVE COMPOUND ANALYSIS AND ANTIOXIDANT ACTIVITY OF ENDOPHYTIC BACTERIAL EXTRACT FROM ADHATHODA BEDDOMEI”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 8, no. 1, Jan. 2015, pp. 70-72, https://innovareacademics.in/journals/index.php/ajpcr/article/view/3457.
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