BIOLOGICAL SYNTHESIS OF KERATIN NANOPARTICLES FROM DOVE FEATHER (COLUMBA LIVIA) AND ITS APPLICATIONS
DOI:
https://doi.org/10.22159/ajpcr.2019.v12i10.34572Keywords:
Dove feathers, Keratin nanoparticles, X-ray diffraction, Scanning electron microscopy, Antibacterial activity, Antioxidant activityAbstract
Objectives: The aim of the present study was to synthesize keratin nanoparticles from dove feathers.
Methods: Crude keratin was extracted by chemical method. The protein content was estimated by Lowry’s method and it was found to be 0.18 mg/ml. The keratin nanoparticles were obtained using glutaraldehyde as cross-linking agent.
Results: A single peak maximum at 270 nm corresponds to the surface plasmon resonance of keratin nanoparticles was observed in the ultraviolet-visible spectrum. The size of keratin nanoparticles was 78 nm. The crystalline size of keratin nanoparticles was 79.6 nm and it was obtained by X-ray diffraction. The antibacterial activity of crude keratin and keratin nanoparticles was determined which revealed that keratin nanoparticles showed higher zone of inhibition than crude keratin protein against Staphylococcus aureus and Salmonella typhi. Keratin nanoparticles showed higher antioxidant activity than crude keratin.
Conclusion: Biological synthesis of nanoparticles has many advantages such as ecofriendly and low cost and can be synthesized in large scale. The keratin nanoparticles can be applied in wound dressing, biosorbent, and cosmetics.
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References
Arun G, Kumar P, Yunus RB, Kamarudin NB. Extraction of Keratin Protein from Chicken feather. Chemeca Engineering a Better World: Sydney Hilton Hotel, NSW, Australia, Conference paper; 2011. p. 18-21.
Zhen LS. Ben Cao Gang Mu. Changchun, Jilin, China: The Time Literature and Art Press; 2005.
Toivola DM, Tao GZ, Habtezion A, Liao J, Omary MB. Cellular integrity plus: Organelle-related and protein-targeting functions of intermediate filaments. Trends Cell Biol 2005;15:608-17.
Kim S, Coulombe PA. Intermediate filament scaffolds fulfill mechanical, organizational, and signaling functions in the cytoplasm. Genes Dev 2007;21:1581-97.
Korniłłowicz-Kowalska T, Bohacz J. Biodegradation of keratin waste: Theory and practical aspects. Waste Manag 2011;31:1689-701.
Grazziotin A, Pimentel FA, De Jong EV, Brandelli A. Nutritional improvement of feather protein by treatment with microbial keratinase. Anim Feed Sci Technol 2006;1261:135-44.
Kumawat TK, Sharma A, Sharma V, Chandra S. Keratin Waste: The Biodegradable Polymers. Doi: 10.5772/intechopen.79502.
Kumaran P, Gupta A, Sharma S. Synthesis of wound-healing keratin hydrogels using chicken feathers proteins and its properties. Int J Pharm Pharm Sci 2007;9:171-8.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951;193:265-75.
Acda MN. Waste chicken feathers as reinforcement in cement bonded composites. Philipp J Sci 2011;139:161-6.
Deivasigamani B, Alagappan KM. Industrial application of keratinase and soluble proteins from feather keratins. J Environ Biol 2008;29:933-6.
Shanmugasundaram OL, Ramkumar M. Characterization and study of physical properties and antibacterial activities of human hair keratin silver nanoparticles and keratin gold nanoparticles coated cotton gauze fabric. J Ind Text 2018;47:798-814.
Meignanalakshmi S, Legadevi R, Banu NA, Gayathri V, Palanisammy A. A study on anti bacterial activity of keratin nanoparticles from chicken feather waste against Staphylococcus aureus (Bovine Mastitis Bacteria) and its anti oxidant activity. Eur J Biotechnol Biosci 2015;3:1-5.
Kunjiappan S, Panneerselvam T, Prasad P, Sukumaran S, Somasundaram B, Sankaranarayanan M, et al. Design, graph theoretical analysis and in silico modeling of Dunaliella bardawil biomass encapsulated keratin nanoparticles: A scaffold for effective glucose utilization. Biomed Mater 2018;13:045012.
Cheng Z, Chen X, Zhai D, Gao F, Guo T, Li W, et al. Development of keratin nanoparticles for controlled gastric mucoadhesion and drug release. J Nanobiotechnology 2018;16:24.
Tran CD, Prosenc F, Franko M, Benzi G. One-pot synthesis of biocompatible silver nanoparticle composites from cellulose and keratin: Characterization and antimicrobial activity. ACS Appl Mater Interfaces 2016;8:34791-801.
Madapally VD, Pandimadevi M. Fabrication of nanofibres by electro spinning using keratin from waste chicken feathers, PVA and AGNPS. Int J Pharm Pharm Sci 2019;11:78-84.
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