THE GREEN SYNTHESIS OF SILVER NANOPARTICLES OF ONION DNA AND SCREENING FOR IN VITRO ANTITYROSINASE ACTIVITY

  • SNEHA THAKUR Department of Pharmacognosy, Bojjam Narasimhulu Pharmacy College for Women, Hyderabad, Telangana, India.
  • KRISHNA MOHAN G Department of Pharmacognosy, Centre for Pharmaceutcial Sciences, Indian Standard Time, Jawaharlal Nehru Technological University, Hyderabad, Telangana, India.

Abstract

Objective: The main objective of the research work is to evaluate the antityrosinase potential of onion DNA silver nanoparticles (AgNPs).


Methods: The onions were procured from the local market and DNA was extracted from onions using detergent and methylated spirit. The isolated DNA was selected for synthesis of AgNPs which acts as capping and reducing agent. About 10 ml of the DNA extract was added to 90 ml of 0.1 N silver nitrate solution. After 24 h incubation, the solution turned dark brown, which indicates the formation of AgNPs. The synthesized DNA AgNPs were characterized by ultraviolet-visible, Fourier transform infrared (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD), and dynamic light scattering (DLS) studies.


Results: The results revealed that the particles were uniform in shape with face-centered cubic structure. The particles are 153±20.4 nm in size and were no signs of agglomeration measured by DLS studies. The FTIR spectroscopy revealed B form of DNA along with strong N-H stretching, C=N stretching, and also asymmetric vibrations of phosphate groups characteristic for DNA molecule. The XRD studies revealed the face-centered cubic structure. SEM studies revealed the spherical structure with average particle size of 150±0.1 nm for single DNA nanoparticles. The onion DNA AgNPs were further investigated for its antityrosinase activity against the standard kojic acid and were to have anticancer potential nearer to the standard.


Conclusion: From the results, it is evident that the synthesized onion DNA AgNPs have antityrosinase potential and can be further investigated for in vivo anticancer potential in future.

Keywords: DNA, Silver nanoparticles, Antityrosinase activity, Kojic acid

References

1. Patil SD, Rhodes DG, Burgess DJ. DNA-based therapeutics and DNA delivery systems: A comprehensive review. AAPS J 2005;7:E61-77.
2. Lo PK, Metera KL, Sleiman HF. Self-assembly of three-dimensional DNA nanostructures and potential biological applications. Curr Opin Chem Biol 2010;14:597-607.
3. Wong KK, Liu X, et al. Silver nanoparticles the real silver bullet in clinical medicine. MedChemComm 2010;1:125-31.
4. Martinez-Gutierrez F, Olive PL, Banuelos A, Orrantia E, Nino N, Sanchez EM, et al. Synthesis, characterization, and evaluation of antimicrobial and cytotoxic effect of silver and titanium nanoparticles. Nanomedicine 2010;6:681-8.
5. Wink M. An Introduction to Molecular Biotechnology: Molecular Fundamentals, Methods and Application in Modern Biotechnology. Weinheim, Germany: Wiley-VCH; 2006.
6. Algar WR, Prasuhn DE, Stewart MH, Jennings TL, Blanco-Canosa JB, Dawson PE, et al. The controlled display of biomolecules on nanoparticles: A challenge suited to bioorthogonal chemistry. Bioconjug Chem 2011;22:825-58.
7. Kasyanenko N, Varshavskii M, Ikonnikov E, Tolstyko E, Belykh R, Sokolov P, et al. DNA modified with metal nanoparticles: Preparation and characterization of ordered Metal-DNA nanostructures in a solution and on a substrate. J Nanomater 2016;2016:3237250.
8. Marta CM, Villamiel M. An Overview of Bioactivity of Onion. New York: Nova Science Publishers, Inc.; 2012.
9. Harper A, Nickels K. DNA Extractions. Australia: Queensland University of Technology; 2008. p. 1-4.
10. Al-Sheddi ES, Farshori NN, Al-Oqail MM, Al-Massarani SM, Saquib Q, Wahab R, et al. Anticancer potential of green synthesized silver nanoparticles using extract of Nepeta deflersiana against human cervical cancer cells (HeLA). Bioinorg Chem Appl 2018;2018:9390784.
11. Ajitha B, Reddy YA, Shameer S, Rajesh KM, Suneetha Y, Reddy PS, et al. Lantana camara leaf extract mediated silver nanoparticles: Antibacterial, green catalyst. J Photochem Photobiol B 2015;149:84-92.
12. Devika R, Elumalai S, Manikandan E, Eswaramoorthy D. Biosynthesis of silver nanoparticles using the fungus Pleurotus ostreatus and their antibacterial activity. Open Access Sci Rep 2012;1:557.
13. Huang J, Li Q, Sun D, Lu Y, Su Y, Yang X, et al. Biosynthesis of silver and gold nanoparticles by novel sundried Cinnamomum camphora leaves. Nanotechnology 2007;18:105104-15.
14. Elassal M, El-Manofy N. Chitosan nanoparticles as drug delivery system for cephalexin and its antimicrobial activity against multiidrug resistent bacteria. Int J Pharm Pharm Sci 2019;11:14-27.
15. Gardouh A, Faheim SH, Solyman SM. Design, optimization and in vitro evaluation of antifungal activity of nanostructured lipid carriers of tolnaftate. Int J Pharm Pharm Sci 2019;11:109-15.
16. Mady M, El-Guendy N, Awad W, Elsayed AA. Interaction of DNA and polyethylamine: Fourier transform infrared (FTIR) differential scanning calorimetry (DSC) studies. Int J Phys Sci 2011;6:7238-334.
17. Volkov IL, Ramazanov RR, Ubyivovk EV, Rolich VI, Kononov AI, Kasyanenko NA, et al. Fluorescent silver nanoclusters in condensed DNA. Chem Phys Chem 2013;14:3543-50.
18. Zheng ZP, Cheng KW, Zhu Q, Wang XC, Lin ZX, Wang M, et al. Tyrosinase inhibitory constituents from the roots of Morus nigra: A structure-activity relationship study. J Agric Food Chem 2010;58:5368-73.
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How to Cite
SNEHA THAKUR, and KRISHNA MOHAN G. “THE GREEN SYNTHESIS OF SILVER NANOPARTICLES OF ONION DNA AND SCREENING FOR IN VITRO ANTITYROSINASE ACTIVITY”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 9, July 2019, pp. 271-5, https://innovareacademics.in/journals/index.php/ajpcr/article/view/34745.
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