• NUZHAT QURESHI Department of Pharmacy, College of Pharmacy, Indore, Madhya Pradesh, India,
  • RAGHVENDRA DUBEY Department of Pharmacy, College of Pharmacy, Indore, Madhya Pradesh, India,
  • NILESH KUMAR PATHAK Department of , Royal Institute of Management and Advanced Studies, Ratlam, Madhya Pradesh, India.


Objective: The objective of this research article is to develop and evaluate polyherbal preparation and comparative studies on diet-induced hyperlipidemia.

Methods: After the extraction, pharmacognostical and phytochemical screening was done. The lipid-lowering activity of polyherbal formulation (T1, T2, T3, T4, and T5) may be attributed to the phytoconstituents present such as alkaloids, carbohydrates, steroids, proteins, tannins, carbohydrates, flavonoids, phenols, glycosides, and triterpenes. In acute oral toxicity study, there were no behavioral changes seen up to 4 h and no mortality was observed up to the end of 24 h even at the maximum tested dose level of 2000 mg/kg per oral. It was considered maximum safe dose. Male and female albino rats weighing 150–200 g were used for the study. Hydroalcoholic extract of all plants was prepared having a dose of 2000 mg/kg. The doses were selected according to the Organisation for Economic Cooperation and Development guideline no. 425. The procedure was divided into two phases: Phase I (observation made on day 1) and Phase II (observed the animals for the next 14 days of drug administration). Animals received a single dose of 2000 mg/kg. After the administration of Healthcare Administration, food was withheld for 3–4 h. If the animal dies, conduct the main test to determine the LD50. The study was conducted by measuring various parameters, namely, daily feed intake (g), water intake (ml), body weight (g), lipid profile high-density lipoprotein (HDL), low-density lipoprotein (LDL), CHL level (mg/dl), and blood glucose level (mg/dl).

Results: Results showed a significant decrease in blood glucose level and serum lipid profile such as total cholesterol, LDL, and increasing serum HDL level, so could be useful in the treatment of hypolipidemia.

Conclusion: Polyherbal formulations (T1, T2, T3, T4, and T5) have hypoglycemic activity and significantly improve lipid profile levels in diet-induced experimental rats.

Keywords: Hyperlipidemia, atherosclerosis, Allium sativum, Moringa olerferus, Cicer arietinum, Hibiscus rosa sinensis, Quisqualis indica


1. Fattepur S, Nilugal CK, Rajendran R, Asmani F, Yusuf E. Anti-hyperlipidemic activity of methanolic extract of Boesenbergia pandurata (finger root) in experimental induced hypercholestrolemic sprague dawley rats. Asian J Pharm Clin Res 2018;11:8.
2. Verma N. Introduction to hyperlipidemia and its treatment: A review. Int J Curr Pharm Res 2017;9:6-14.
3. Nouh F, Omar M, Younis M. Risk factors and management of hyperlipidemia (review). Asian J Cardiol Res 2019;2:1-10.
4. Venkateshan S, Subramaniyan V, Chinnasamy V, Chandiran S. Anti-oxidant and anti-hyperlipidemic activity of Hemidesmus indicus in rats fed with high-fat diet. Avicenna J Phytomed 2016;6:516-25.
5. Iyer A, Panchal S, Poudyal H, Brown L. Potential health benefits of Indian spices in the symptoms of the metabolic syndrome: A review. Indian J Biochem Biophys 2009;46:467-81.
6. Lankin VZ, Tikhaze AK, Kukharchuk VV, Konovalova GG, Pisarenko OI, Kaminnyi AI, et al. Antioxidants decreases the intensification of low density lipoprotein in vivo peroxidation during therapy with statins. Mol Cell Biochem 2003;249:129-40.
7. Sarvesh CN, Fernandes J. Evaluation of antihyperlipidemic activity of leaves of Achyranthes aspera Linn. Using hyperlipidemic rats. Asian J Pharm Clin Res 2017;10:211-5.
8. Mamun MA, Hasan N, Shirin F, Belal MH, Khan MA, Tasnin MN, et al. Antihyperglycemic and antihyperlipidemic activity of ethanol extract of garlic (Allium sativum) in streptozotocin-induced diabetic mice. Int J Med Health Res 2017;3:63-6.
9. Keshetty V, Pabba S, Gudipati R, Kandukuri JM, Allenki V. Antihyperlipidemic activity of methanolic extract of garlic (Allium sativum L.) in triton X-100 induced hyperlipidemic rats. J Pharm Res 2009;2:777-80.
10. Jain RC, Vyas CR. Garlic and alloxan induced diabetic rabbits. Am J Clin Nutr 1975;28:684-5.
11. Bais S, Singh GS, Sharma R. Antiobesity and hypolipidemic activity of Moringa oleifera leaves against high fat diet-induced obesity in rats. Adv Biol 2014;2014:157895.
12. Jain PG, Patil SD, Haswani NG, Girase MV, Surana SJ. Hypolipidemic activity of Moringa oleifera Lam., moringaceae, on high fat diet induced hyperlipidemia in albino rats. Braz J Pharmacogn 2010;20:969-73.
13. Harini S, Adilaxmamma K, Mohan EM, Srilatha C, Raj MA. Antihyperlipidemic activity of chickpea sprouts supplementation in ovariectomy induced dyslipidemia in rats. J Ayurveda Integr Med 2015;6:104-10.
14. Sikarwar MS, Patil MB. Antihyperlipidemic activity of Hibiscus rosa-sinensis Linn. ethanolic extract fractions. Int J Health Allied Sci 2015;4:73-8.
15. Jyoti S, Kumar PP, Kaur CD. Effect of Quisqualis indica extract on cholesterol diet induced hyperlipidemia in rats. Res J Pharmacol Pharmacodyn 2013;5:317-20.
16. Sahu JJ, Patel PK, Dubey B. Effects of methanolic extracts of Quisqualis indica (aerial parts) on passive smoking induced hyperlipidemia in rats. Asian J Pharm Tech 2013;3:26-9.
17. Bairagi VA, Sadu N, Senthilkumar KL, Ahir Y. Antidiabetic potential of Quisqualis indica linn in rats. Int J Pharm Phytopharmacol Res 2012;1:16671.
18. Bisht A, Madhav NV, Upadhyaya K. Screening of polyherbal formulation for its potential anti-hyperlipidemic and antioxidant activity. J Pharmacogn Phytochem 2015;3:134-9.
19. Sharma S, Khare S, Dubey BK, Joshi A, Jain A. Analgesic activity of polyherbal formulation in experimental rats by acetic acid induced hot plate model. J Drug Deliv Ther 2019;9:276-80.
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How to Cite
NUZHAT QURESHI, RAGHVENDRA DUBEY, and NILESH KUMAR PATHAK. “POLYHERBAL PREPARATION AND COMPARATIVE STUDIES ON DIET-INDUCED HYPERLIPIDEMIA”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 12, Nov. 2019, pp. 190-6, doi:10.22159/ajpcr.2019.v12i12.34872.
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