PROTECTIVE EFFECT OF PHYLLANTHUS EMBLICA EXTRACT PREVENT CONTRAST-INDUCED ACUTE KIDNEY INJURY IN RATS

  • SUPRANEE KONGKHAM Department of Preclinical Sciences, Division of Biochemistry, Faculty of Medicine, Thammasat University (Rangsit Campus), Pathumthani, Thailand.
  • ADIS TASANARONG Department of Medicine, Nephrology Unit, Faculty of Medicine, Thammasat University (Rangsit Campus), Pathumthani, Thailand.
  • ARUNPORN ITHARAT Division of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University (Rangsit Campus), Pathumthani, Thailand.

Abstract

Objective: The objective of the study was to investigate the anti-apoptosis effect of the extract from Phyllanthus emblica (PE) for the prevention of contrast-induced acute kidney injury (CI-AKI).


Methods: Male Sprague Dawley rats were given saline (control) or PE extracts (500 mg/kg/day) for 5 days before the induction of CI-AKI. Renal tissues were collected for an evaluation of gene expression and immunohistochemistry (IHC). To indicate anti-apoptotic effect, the expression levels of Bax, Bcl-2, and caspase in kidney were also determined, using real-time polymerase chain reaction (RT-PCR) and Western blot analysis.


Results: In the CI-AKI group, RT-PCR and Western blot analysis revealed that the expression levels of Bax and cleaved-caspase-3 were upregulated in the CI-AKI group, whereas the expression of Bcl-2 was downregulated. However, the pre-treatment with PE increased Bcl-2 expression. Moreover, decreased cleaved-caspases-3 activity was also detected using IHC.


Conclusion: These findings suggested that pretreatment with PE extract provided the anti-apoptotic effect against CI-AKI in the rat model.

Keywords: Anti-apoptosis, Contrast-induced acute kidney injury, Phyllanthus emblica

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SUPRANEE KONGKHAM, ADIS TASANARONG, and ARUNPORN ITHARAT. “PROTECTIVE EFFECT OF PHYLLANTHUS EMBLICA EXTRACT PREVENT CONTRAST-INDUCED ACUTE KIDNEY INJURY IN RATS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 10, Aug. 2019, pp. 197-01, https://innovareacademics.in/journals/index.php/ajpcr/article/view/35042.
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