IN VITRO EVALUATION OF ANTIDIABETIC AND CYTOTOXICITY POTENTIALS OF THE RHIZOME EXTRACT OF DRYNARIA QUERCIFOLIA (L.) J. SMITH

  • PRASANNA G Department of Biochemistry, Sengamala Thayaar Educational Trust Women’s College, Thiruvarur, Tamil Nadu, India.
  • DEVI R Department of Biochemistry, Sengamala Thayaar Educational Trust Women’s College, Thiruvarur, Tamil Nadu, India.
  • ISHWARYA G Department of Biochemistry, Sengamala Thayaar Educational Trust Women’s College, Thiruvarur, Tamil Nadu, India.

Abstract

Objective: In the present study, an attempt has been made to evaluate the in vitro antidiabetic and cytotoxic potentials of the rhizome extract of Drynaria quercifolia (L.) J. Smith.


Methods: In vitro antidiabetic activity was determined by two different assays such as alpha-amylase inhibition assay and glucose uptake assay. The plant extract with three different concentrations was used for this assay. L6 rat myogenic cells were selected and subjected to glucose uptake assay. The cytotoxic activity of the different concentrations of the plant extract on HepG2 cell line was also investigated in vitro through 3-(4,5, dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay.


Results: The findings of the study provide evidence that the rhizome extract of D. quercifolia possesses significant anti-diabetic activity. In MTT assay, the significant cytotoxic effect of plant extract was observed by measuring the percentage of cell viability on the HepG2 cell line.


Conclusion: The findings indicated that rhizome extracts of D. quercifolia have potential as a medicinal drug against diabetes mellitus (DM) and liver cancer. Further, studies with in vivo and clinical trials need to be conducted to establish rhizome extract as a safe agent for DM and liver cancer therapy.

Keywords: Anticancer, Antidiabetic, Cytotoxicity, Drynaria quercifolia, HepG2, L6 rat myogenic cells, 3-(4,5, dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, Rhizome extract

References

1. Kumar PJ. Clark M. Textbook of Clinical Medicine. London: Saunders; 2002. p. 1099-121.
2. Ahmed AM. History of diabetes mellitus. Saudi Med J 2002;23:373-8.
3. Ford ES, Li C, Zhao G. Prevalence and correlates of metabolic syndrome based on a harmonious definition among adults in the US. J Diabetes 2010;2:180-93.
4. Jahan S, Fariduddin M, Sultana N, Aktar Y, Hasan M. Predictors of post-partum persistence of glucose intolerance and its association with cardio-metabolic risk factors in gestational diabetes mellitus. J Diabetes Metab 2015;6:609.
5. Das A, McGuire PG, Monickaraj F. Novel pharmacotherapies in diabetic retinopathy: Current status and what’s in the horizon? Indian J Ophthalmol 2016;64:4-13.
6. Ghosh S, Suryawanshi SA. Effect of Vinca rosea extracts in treatment of alloxan diabetes in male albino rats. Indian J Exp Biol 2001;39:748-59.
7. Majid DG, Iqbal M. Antihyperglycemic and antioxidative effects of Lygodium microphyllum in alloxan induced diabetic rats. Int J Pharm Pharm Sci 2018;10:75-9.
8. Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, et al. Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer 2015;136:E359-86.
9. Crissien AM, Frenette C. Current management of hepatocellular carcinoma. Gastroenterol Hepatol (N Y) 2014;10:153-61.
10. Stopeck AT, Thompson PA. Breast Cancer Treatment and Management. WebMD Health Professional Network; 2012. Available from: http:// www.emedicine.medscape.com/article/1947145-treatment.
11. Lachenmayer A, Alsinet C, Chang CY, Llovet JM. Molecular approaches to treatment of hepatocellular carcinoma. Dig Liver Dis 2010;42 Suppl 3:S264-72.
12. Cumming R, Sked J. Drynaria and Platycerium: Interesting fern genera. Soc Growing Aust Plants 2006;27:2011.
13. Prasanna G, Chitra M. Phytochemical screening and GC-MS analysis of Drynaria quercifolia rhizome. Am J Adv Drug Deliv 2015;3:72-8.
14. Prasanna G, Chitra M, Suvitha N. In vitro antimicrobial activity of Drynaria quercifolia L. rhizome. Asian J Biochem Pharm Res 2014;4:342-9.
15. Nejad BS, Deokule SS. Anti-dermatophytic activity of Drynaria quercifolia (L.) J. Smith, Jundishapur. J Microbiol 2009;2:25-30.
16. Mohanta MC, Ganguly A, Begum SM, Rahman A. Evaluation of anti-nociceptive properties of Drynaria quercifolia rhizome in Swiss-albino mice. J Pharm Res 2014;8:41-4.
17. Prasanna G, Chitra M. In vitro antioxidant activity of Drynaria quercifolia L. Rhizome. Int J Pharm Sci Res 2015;6:3061-6.
18. Prasanna G, Anuradha R. Evaluation of in vitro antioxidant activity of rhizome extract of Drynaria quercifolia L. Int J ChemTech Res 2015;8:183-7.
19. Devika V, Prasanna G. In vitro hepatoprotective activity of Drynaria quercifolia L. Rhizome. World J Pharm Pharm Sci 2016;5:512-21.
20. Janaranjani B, Prasanna G, Chitra M. Anti-inflammatoryand antipyretic activities of Drynaria quercifolia rhizome in rats. Int J Pharm Sci Rev Res 2014;29:57-61.
21. Anuja GI, Latha PG, Suja SR, Shyamal S, Shine VJ, Sini S, et al. Anti-inflammatory and analgesic properties of Drynaria quercifolia (L.) J. Smith. J Ethnopharmacol 2010;132:456-60.
22. Soni DK, Mohan AJ, Mantena VR, Raju K. Antiulcer activity of ethanolic extract of Drynaria quercifolia Linn. Leaves. J Pharm Res 2012;5:117-9.
23. Miller GL. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 1959;31:426-8.
24. Sundarrajan T, Velmurugan V, Srimathi R. Phytochemical evaluation and in vitro antidiabetic activity of ethanolic extract of Alternanthera ficodia Linn. Res J Pharm Technol 2017;10:2981-3.
25. Masters RW. Animal Cell Culture, Cytotoxicity and Viability Assays. 3rd ed. Oxford University Press: Oxford; 2000.
26. Patel DK, Kumar R, Prasad SK, Sairam K, Hemalatha S. Antidiabetic and in vitro antioxidant potential of Hybanthus enneaspermus (Linn) F. Muell in streptozotocin-induced diabetic rats. Asian Pac J Trop Biomed 2011;1:316-22.
27. Patel DK, Prasad SK, Kumar R, Hemalatha S. An overview on antidiabetic medicinal plants having insulin mimetic property. Asian Pac J Trop Biomed 2012;2:320-30.
28. Saxena A, Vikram NK. Role of selected Indian plants in management of Type 2 diabetes: A review. J Altern Complement Med 2004;10:369-78.
29. Bhalodi M., Shukla Saluja AK. In vitro antioxidant activity of the flowers of Ipomoea aquatica forsk. Pharmacogn Mag 2008;4:220-6.
30. Bhandari MR, Anurakkun NJ, Hong G, Kawabata J. Alpha glucosidase and alpha amylase inhibitory activities of Nepalese medicinal herb Pakhanbhed (Bergenia ciliata, Haw). Food Chem 2008;106:247-52.
31. Jung M, Park M, Lee HC, Kang YH, Kang ES, Kim SK, et al. Antidiabetic agents from medicinal plants. Curr Med Chem 2006;13:1203-18.
32. Viswanathan MB. Industries Meet Cum Seminar on Biodiversity and Information on Medicinal and Aromatic Plants. New Delhi: NISCOM; 1995. p. 60.
33. Chopra RN, Nayar SL, Chopra IC. Glossary of Indian Medicinal Plants. New Delhi: Council of Scientific and Industrial Research India; 1956. p. 57, 102, 139, 198, 256.
34. Lebovitz HE. Alpha-glucosidase inhibitors as agents in the treatment of diabetes. Diabetes Rev 1998;6:132-45.
35. Scott LJ, Spencer CM. Miglitol: A review of its therapeutic potential in Type 2 diabetes mellitus. Drugs 2000;59:521-49.
36. Ali H, Houghton PJ, Soumyanath A. Alpha-amylase inhibitory activity of some malaysian plants used to treat diabetes; with particular reference to Phyllanthus amarus. J Ethnopharmacol 2006;107:449-55.
37. Conforti F, Statti G, Loizzo MR, Sacchetti G, Poli F, Menichini F, et al. In vitro antioxidant effect and inhibition of alpha-amylase of two varieties of Amaranthus caudatus seeds. Biol Pharm Bull 2005;28:1098-102.
38. Loizzo MR, Tundis R, Menichini F, Saab AM, Statti GA, Menichini F, et al. Cytotoxic activity of essential oils from labiatae and lauraceae families against in vitro human tumor models. Anticancer Res 2007;27:3293-9.
39. Srividya AR, Dhanabal P, Bavadia P, Vishnuvarthan VJ, Kumar MN. Antioxidant and antidiabetic activity of Curcuma aromatica. Int J Res Ayurveda Pharm 2012;3:401-5.
40. Yeh GY, Eisenberg DM, Kaptchuk TJ, Phillips RS. Systematic review of herbs and dietary supplements for glycemic control in diabetes. Diabetes Care 2003;26:1277-94.
41. Rang HP, Dale MM, Ritter JM, and Flower RJ. The Endocrine Pancreas and the Control of Blood Glucose in Rang and Dales Pharmacology. 6th ed. Edinburgh: Church Livingstone 2007. p. 397-409.
42. Nolte M. Pancreatic hormones and antidiabetic drugs. In: Katzung BG, Masters SB, Trevor AJ, editors. Basic and Clinical Pharmacology. 11th ed. New Delhi: McGraw-Hill Education; 2009. p. 727-51.
43. Ramesh C, Rani AP. In vivo and in vitro evaluation of Tephrosia calophylla for anti-diabetic properties. Int J Pharm Pharm Sci 2018;10:138-44.
44. Reaven G, Abbasi F, McLaughlin T. Obesity, insulin resistance, and cardiovascular disease. Recent Prog Horm Res 2004;59:207-23.
45. Kumar M, Zhao X, Wang XW. Molecular carcinogenesis of hepatocellular carcinoma and intrahepatic cholangiocarcinoma: One step closer to personalized medicine? Cell Biosci 2011;1:5.
46. Tanaka H, Fujita N, Sugimoto R, Urawa N, Horiike S, Kobayashi Y, et al. Hepatic oxidative DNA damage is associated with increased risk for hepatocellular carcinoma in chronic hepatitis C. Br J Cancer 2008;98:580-6.
47. Rao MR, Adagale UR, Shetty A, Namjoshi P, Gaitonde P, Jain P. Cancer Immunotherapy; 2007. Available from: http://www.pharmainfo.net/ reviews/cancer immunotherapy.
48. Cragg GM, Newman DJ. Antineoplastic agents from natural sources: Achievements and future directions. Expert Opin Investig Drugs 2000;9:2783-97.
49. Newman DJ, Cragg GM, Snader KM. Natural products as sources of new drugs over the period 1981-2002. J Nat Prod 2003;66:1022-37.
50. Govind P, Sahni YP. Phytotherapy of malnutritional cancers in animals. Int J Pharm Sci Rev Res 2011;8:14-20.
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PRASANNA G, DEVI R, and ISHWARYA G. “IN VITRO EVALUATION OF ANTIDIABETIC AND CYTOTOXICITY POTENTIALS OF THE RHIZOME EXTRACT OF DRYNARIA QUERCIFOLIA (L.) J. SMITH”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 11, Sept. 2019, pp. 72-76, doi:10.22159/ajpcr.2019.v12i11.35078.
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