CONDITIONED MEDIA OF MESENCHYMAL STEM CELLS RECOVERS PLACENTAL GROWTH FACTOR IN PRISTANE MICE MODEL

  • MUHAMMAD ADRIANES BACHNAS Doctorate Program in Medical Sciences, Universitas Sebelas Maret, Solo, Indonesia.
  • AMBAR MUDIGDO Doctorate Program in Medical Sciences, Universitas Sebelas Maret, Solo, Indonesia.
  • BAMBANG PURWANTO Doctorate Program in Medical Sciences, Universitas Sebelas Maret, Solo, Indonesia.
  • SRI SULISTYOWATI Department of Obstetrics and Gynecology, Division of Maternal-Fetal Medicine, Faculty of Medicine, Universitas Sebelas Maret, Dr. Moewardi Hospital, Solo, Indonesia.

Abstract

Objectives: A low level of placental growth factor (PlGF) expression in placenta is strongly correlated with pregnancy loss, prematurity, fetal growth restriction, and preeclampsia. Pristane-induced lupus in mice model has been widely used to present placental damage and poor pregnancy outcomes. Conditioned media (CM), which is medium for culturing mesenchymal stem cells (MSCs), have grown much attention recently. It has rich amount of growth factors, exosomes, microvesicles, and immunomodulatory molecules. These could become beneficial resources to recover poor placentation.


Methods: CM taken from the 3rd to 4th passage of the culture were underdone by using explant method of human umbilical cord (hUC). Ninety BALB/c mice were randomly distributed into three groups: Normal, pristane, and pristane + CM group. Mice in normal group were injected with 0.5 ml normal saline and other groups with 0.5 ml pristane. Four weeks later, mice were mated and only pristane + CM group received therapy of CM-MSCs 0.5 ml single dose after pregnancy was confirmed. On day 16, pregnancy was terminated and the placenta was analyzed for PlGF expression using immunoreactive score and statistically tested with analysis of variance.


Results: The average of PlGF expression in normal, pristane, and pristane + CM group was 9.528, 3.428, and 10.085, respectively. CM have significantly increase PlGF (p<0.01**) and equal to normal (p=0.301).


Conclusion: CM of hUC-MSCs has shown its therapeutical effect among the low level of PlGF in damaged placenta, and potentially would prevent fetal growth restriction and preeclampsia arose from spiral artery remodeling failure.

Keywords: Conditioned media, Mesenchymal stem cells, Placental growth factor

Author Biographies

AMBAR MUDIGDO, Doctorate Program in Medical Sciences, Universitas Sebelas Maret, Solo, Indonesia.

Professor Ambar Mudigdo

      Head of Department of Pathology Anatomy,

      Faculty of Medicine, Universitas Sebelas Maret,

      Dr. Moewardi Hospital, Solo - Indonesia

      Doctorate Program in Medical Sciences, Postgraduate-Universitas Sebelas Maret

      Phone: +628122600040

      email: ambar.mudigdo@yahoo.com

BAMBANG PURWANTO, Doctorate Program in Medical Sciences, Universitas Sebelas Maret, Solo, Indonesia.

Professor Bambang Purwanto

      Head of Department of Internal Medicine

      Faculty of Medicine, Universitas Sebelas Maret,

      Dr. Moewardi Hospital, Solo - Indonesia

      Doctorate Program in Medical Sciences, Postgraduate-Universitas Sebelas Maret

      Phone: +628122988079

      email: bambangp.solo@yahoo.com

SRI SULISTYOWATI, Department of Obstetrics and Gynecology, Division of Maternal-Fetal Medicine, Faculty of Medicine, Universitas Sebelas Maret, Dr. Moewardi Hospital, Solo, Indonesia.

Professor Sri Sulistyowati

     Full-time Professor

      Maternal-Fetal Medicine Division,

      Department of Obstetrics and Gynecology,

      Faculty of Medicine, Universitas Sebelas Maret,

      Dr. Moewardi Hospital, Solo - Indonesia

      Phone: +628122968215

      email: elis_spog@yahoo.co.id

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MUHAMMAD ADRIANES BACHNAS, AMBAR MUDIGDO, BAMBANG PURWANTO, and SRI SULISTYOWATI. “CONDITIONED MEDIA OF MESENCHYMAL STEM CELLS RECOVERS PLACENTAL GROWTH FACTOR IN PRISTANE MICE MODEL”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 10, Sept. 2019, pp. 242-4, https://innovareacademics.in/journals/index.php/ajpcr/article/view/35198.
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