GASTROPROTECTIVE ACTIVITY OF SESAME (SESAMUM INDICUM) SEEDS EXTRACT IN ALENDRONATE INDUCED GASTRIC ULCER IN RATS.

  • Balaji Ommurugan Post graduate , Department of Pharmacology, Kasturba Medical college , Manipal university , Manipal.
  • Vanishree Rao Post graduate, Department of Pharmacology, Kasturba Medical College, Manipal Academy of Higher Education, Manipal.
  • Shubhangi Mishra Post graduate, Department of Pharmacology, Kasturba Medical College, Manipal Academy of Higher Education, Manipal.
  • Shalini Adiga 3. Professor, Department of Pharmacology, Kasturba Medical College, Manipal Academy of Higher Education, Manipal

Abstract

ABSTRACT


Background: Bisphosphonates are known to cause gastritis and only few studies have evaluated the use of plant extracts in the treatment of bisphosphonate induced peptic ulcers.   In the present study, we examined the effect of sesame seed extract in the alendronate-induced gastric ulcer in adult Wistar rats.


Methods: Thirty adult Wistar rats (180-250 g) of both sexes, divided into five groups (N=6) were used. Group 1 (vehicle control)-0.9% saline, Group 2 (ulcer control)- Alendronate 60mg/kg, Group 3 (standard)- Alendronate 60mg/kg + Pantoprazole 30mg/kg/day, Group 4 (test groups)- Alendronate 60mg/kg + Sesame 0.5mg/kg, Group 5 (test groups) – Alendronate 60mg/kg + Sesame 1mg/kg. All drugs given orally once daily for 7 days except for alendronate given only on day one. On day 8, rats were sacrificed, and stomach tissues were analysed macroscopically for ulcers. Estimation of gastric pH, acidity and volume was done along with mucin content measurement. One- way Anova followed by Tukey’s post hoc test was used for statistical analysis with p value (<0.05) taken as significant.


Results: Our study found that sesame extract given therapeutically at doses of 0.5 and 1 mg/kg showed comparable results with that of pantoprazole 30mg/kg. Both the doses of sesame, 0.5 & 1mg/kg showed similar gastroprotective activity against alendronate induced gastric ulcers in Wistar rats.


Conclusion: The findings of this study clearly demonstrate the protective effects of Sesame against Alendronate-induced gastric ulceration.


Key words: Gastroprotection, Sesame, alendronate, gastric ulcers.


 

Keywords: Gastroprotection, Sesame, alendronate, gastric ulcers.

References

1. Cai S, García Rodríguez LA, Massó-González EL and Hernández-Díaz S. Uncomplicated peptic ulcer in the UK: trends from 1997 to 2005. Aliment Pharmacol. Ther. (2009) 30: 1039-1048.
2. Malfertheiner P, Chan FK, McColl KE. Peptic ulcer disease “The Lancet” 2009;374(9699):1449–1461.
3. Fleisch H. Bisphosphonates in Bone Disease: From the Laboratory to the Patient. New York: The Parthenon Group; 1997: 32–163
4. Chesnut CH, McClung MR, Ensrud KE, Bell NH, Genant HK, Harris ST, et al. Alendronate treatment of the postmenopausal osteoporotic woman: effect of multiple dosages on bone mass and bone remodelling. The American journal of medicine. 1995 Aug 1;99(2):144-52.
5. De Groen PC, Lubbe DF, Hirsch LJ, Daifotis A, Stephenson W, Freedholm D, et al. Esophagitis associated with the use of alendronate. New England Journal of Medicine. 1996 Oct 3;335(14):1016-21.
6. Graham DY, Malaty HM. Alendronate gastric ulcers. Alimen. Pharmacol Ther. 1999; 13:515–519.
7. Graham DY. What the gastroenterologist should know about the gastrointestinal safety profiles of bisphosphonates. Dig Dis Sci. 2002; 47:1665–1678.
8. Ohashi Y, Aihara E, Takasuka H, Takahashi K, Takeuchi K. Antral ulcers induced by alendronate, a nitrogen-containing biphophonate, in rat stomachs–prophylactic effect of rebamipide. J Physiol Pharmacol 2009; 60: 85–93.
9. Silva RO, Lucetti LT, Wong DV, Aragão KS, Junior EM, Soares PM, et al. Alendronate induces gastric damage by reducing nitric oxide synthase expression and NO/cGMP/K ATP signaling pathway. Nitric Oxide. 2014 Aug 31; 40:22-30.
10. Shinohara T, Takahashi N, Kohno H, et al. Mitochondria are targets for geranylgeranylacetone-induced cardioprotection against ischemia-reperfusion in the rat heart. Am J Physiol Heart Circ Physiol 2007; 293: H1892–H1899.
11. Nagano Y, Matsui H, Muramatsu M, et al. Rebamipide significantly inhibits indomethacin-induced mitochondrial damage, lipid peroxidation, and apoptosis in gastric epithelial RGM-1 cells. Dig Dis Sci 2005; 50 (Suppl 1): S76–S83
12. Wallace JL, Dicay M, McKnight W, Bastaki S, Blank MA. N-bisphosphonates cause gastric epithelial injury independent of effects on the microcirculation. Alimentary Pharmacology and Therapeutics. 1999 Dec 1;13(12):1675-82.
13. Hsu DZ, Chen KT, Li YH, Chuang YC, Liu MY: Sesamol delays mortality and attenuates hepatic injury after cecal ligation and puncture in rats: role of oxidative stress. Shock 25:528Y532, 2006.
14. Amagase K, Hayashi S, Nishikawa K, Aihara E, Takeuchi K. Impairment of gastric ulcer healing by alendronate, a nitrogen-containing bisphosphonate, in rats. Digestive diseases and sciences. 2007 Aug 1;52(8):1879-89.
15. Lee HJ, Han YM, Kim EH, Kim YJ, Hahm KB. A possible involvement of Nrf2-mediated heme oxygenase-1 up-regulation in protective effect of the proton pump inhibitor pantoprazole against indomethacin-induced gastric damage in rats. BMC gastroenterology. 2012 Oct 16;12(1):143.
16. Hsu DZ, Chen YW, Chu PY, Periasamy S, Liu MY. Protective effect of 3, 4-methylenedioxyphenol (sesamol) on stress-related mucosal disease in rats. BioMed research international. 2013 Jul 25;2013.
17. Rao CM, Ramesh KU, Bairy KL, Kulkarni OR. Indian drugs. 1990:28;64-7.
18. Khayyal MT, El-Ghazaly MA, Kenawy SA, Seif-El-Nasr M, Mahran LG, Kafafi YA. Antiulcerogenic effect of some gastrointestinally acting plant extracts and their combination. Arzneimittelforschung. 2001 Jul;51(07):545-53.
19. Anoop A, Jegadeesan M. Biochemical studies on the anti-ulcerogenic potential of Hemidesmus indicus R. Br. var. indicus. Journal of ethnopharmacology. 2003 Feb 28;84(2):149-56.
20. Elliott SN, McKnight W, Davies NM, MacNaughton WK, Wallace JL. Alendronate induces gastric injury and delays ulcer healing in rodents. Life sciences. 1997 Nov 28;62(1):77-91.
21. Peter CP, Handt LK, Smith SM. Esophageal irritation due to alendronate sodium tablets (possible mechanisms). Digestive diseases and sciences. 1998 Sep 1;43(9):1998-2002.
22. Graham DY, Malati HM, Goodgame R. Primary amino-bisphosphonates: a new class of gastrotoxic drugs – comparison of alendronate and aspirin. Am J Gastroenterol 1997;92: 1322-5.
23. Morris JB. Food, industrial, nutraceutical, and pharmaceutical uses of sesame genetic resources. Trends in new crops and new uses. 2002:153-6.
24. Sirato-Yasumoto S, Katsuta M, Okuyama Y, Takahashi Y, Ide T. Effect of sesame seeds rich in sesamin and sesamolin on fatty acid oxidation in rat liver. Journal of Agricultural and Food Chemistry. 2001 May 21;49(5):2647-51.
25. Anilkumar, K., Pal, A., Khanum, F., Bawa, A. 2010. Nutritional, Medicinal and Industrial Uses of Sesame (Sesamum indicum L.) Seeds - An Overview. Agriculturae Conspectus Scientificus ;75(4): 159-168.
26. Hsu DZ et al. Protective Effect of 3,4-Methylenedioxyphenol (Sesamol) on Stress-Related Mucosal Disease in Rats. BioMed Research International 2013; Article ID 481827.
27. Hsu DZ, Chu PY, Liu MY. Effect of Sesame Oil on Acidified Ethanol-Induced Gastric Mucosal Injury in Rats. Journal of Parenteral and Enteral Nutrition. 2009;33 (4): 423 – 427.
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How to Cite
Ommurugan, B., V. Rao, S. Mishra, and S. Adiga. “GASTROPROTECTIVE ACTIVITY OF SESAME (SESAMUM INDICUM) SEEDS EXTRACT IN ALENDRONATE INDUCED GASTRIC ULCER IN RATS.”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 10, Sept. 2019, pp. 214-7, https://innovareacademics.in/journals/index.php/ajpcr/article/view/35203.
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