Chemical Constituents, Anti-Oxidative, Cytotoxic and Genotoxic Effects of Miscathus Capensis Roots Extract.

  • IDOWU JONAS SAGBO School of Biology and Environmental Sciences, University of Mpumalanga, Private Bag X11283, Mbombela, 1200, South Africa.
  • WILFRED OTANG MBENG School of Biology and Environmental Sciences, University of Mpumalanga, Private Bag X11283, Mbombela, 1200, South Africa.


Objective: Miscanthus capensis (Nees) Andersson (Poaceae family) is used in traditional medicine to treat pimples, wounds, eczema, acne, and other ailments. The present study investigates the possible chemical constituents, antioxidant, cytotoxic, and genotoxic effects of the methanol extract of the M. capensis roots.

Methods: Gas chromatography-mass spectrometry (GC-MS) analysis was carried out to identify the chemical constituents of the plant extract. Cytotoxicity and mode of cell death toward human dermal fibroblasts (MRHF) cells were assessed using the ImageXpress® Micro XLS analysis system. Genotoxic effect toward Vero cells was also investigated using micronucleus assay. Furthermore, ferric reducing power (Ferric reducing antioxidant potential [FRAP]) and reactive oxygen species (ROS) levels in MRHF cells were used to estimate the antioxidant activity of the plant extract.

Results: The GC-MS results showed a maximum amount of bioactive components (77 compounds) with rosifoliol (33.66%) being the major component detected. However, the extract was not toxic to MRHF cells but demonstrated anti-proliferation rather than cell death at the tested concentrations (25, 50, and 100 μg/ml). In addition, the plant extract also displayed non-genotoxic toward Vero cells with no significant increase in micronucleus formation at the tested concentration while a concentration dependence response in FRAP values and ROS levels in MRHF cells lines were observed indicating better antioxidant activities of the plant extract.

Conclusion: The results suggest that M. capensis could be a promising candidate for preventing or eradicating skin and oxidative-stress related diseases.

Keywords: Antioxidant, Poaceae, Oxidative stress, Skin


1. Luo H, Yamamoto Y, Liu Y, Jung JS, Kahng HY, Koh YJ, et al. The in vitro antioxidant properties of Chinese highland lichens. J Microbiol Biotechnol 2010;20:1524-8.
2. Hwang HS, Winkler Moser JK, Liu SX. Structural Polymerization, effect of lignans and sesamol on Temperature of soybean oil at frying. J Am Oil Chem Soc 2012;89:1067-76.
3. Choi JS, Chung HY, Jung HA, Park HJ, Yokozawa T. Comparative evaluation of antioxidant potential of alaternin (2-hydroxyemodin) and emodin. J Agric Food Chem 2000;48:6347-51.
4. Gupta-Elera G, Garrett AR, Robison RA, O’Neill KL. The role of oxidative stress in prostate cancer. Eur J Cancer Prev 2012;21:155-62.
5. Stohs SJ. The role of free radicals in toxicity and disease. J Basic Clin Physiol Pharmacol 1995;6:205-28.
6. Siddiqui IA, Adhami VM, Saleem M, Mukhtar H. Beneficial effects of tea and its polyphenols against prostate cancer. Mol Nutr Food Res 2006;50:130-43.
7. Costa DA, de Oliveira GA, Lima TC, dos Santos PS, de Sousa DP, de Freitas RM, et al. Anticonvulsant and antioxidant effects of cyano-carvone and its action on acetylcholinesterase activity in mice hippocampus. Cell Mol Neurobiol 2012;32:633-40.
8. da Rocha ML, Oliveira LE, Patrício Santos CC, de Sousa DP, de Almeida RN, Araújo DA, et al. Antinociceptive and anti-inflammatory effects of the monoterpene ?,?-epoxy-carvone in mice. J Nat Med 2013;67:743-9.
9. Jafari S, Saeidnia S, Abdollahi M. Role of natural phenolic compounds in cancer chemoprevention via regulation of the cell cycle. Curr Pharm Biotechnol 2014;15:409-21.
10. Unnati S, Ripal S, Sanjeev A, Niyati A. Novel anticancer agents from plant sources. Chin J Nat Med 2013;11:16-23.
11. Sagbo IJ, Otang Mbeng W. Plants used for cosmetics in the Eastern Cape Province of South Africa: A case study of skin care. Phcogn Rev 2018;12:139-56.
12. Odeyemi S. A Comparative Study of the in vitro Antidiabetic Properties, Mechanism of Action and Cytotoxicity of Albuca setosa and Albuca bracteata Bulb Extracts. [PhD Thesis]. Department of Biochememistry and Microbiology: University of Fort Hare; 2016.
13. Lima LA, Johann S, Cisalpino PS, Pimenta LP, Boaventura MA. In vitro antifungal activity of fatty acid methyl esters of the seeds of Annona cornifolia A.St.-Hil. (Annonaceae) against pathogenic fungus Paracoccidioides brasiliensis. Rev Soc Bras Med Trop 2011;44:777-80.
14. Ho CL, Liao PC, Wang EI, Su YC. Composition and antifungal activities of the leaf essential oil of Neolitsea parvigemma from Taiwan. Nat Prod Commun 2011;6:1357-60.
15. Tung YT, Huang CC, Ho ST, Kuo YH, Lin CC, Lin CT, et al. Bioactive phytochemicals of leaf essential oils of Cinnamomum osmophloeum prevent lipopolysaccharide/D-galactosamine (LPS/D-GalN)-induced acute hepatitis in mice. J Agric Food Chem 2011;59:8117-23.
16. Wieczorek P. Structure of natural antibiotic CP-47,444. Chemik 2006;59:55-9.
17. Dandekar R, Fegade B, Bhaskar VH. GC-MS analysis of phytoconstituents in alcohol extract of Epiphyllum oxypetalum leaves. J Pharmacogn Phytochem 2015;4:149-54.
18. Soosairaj S, Dons T. Bio-active compounds analysis and characterization in ethanolic plant extracts of Justicia tranquebariensis L. (Acanthaceae)- using GC-MS. Int J ChemTech Res 2016;9:260-5.
19. Hase JG, Deshumkh KK, Pokharkar DR, Gaje RT, Phatanagre DN. Phytochemical studies on Nerium oleander L. using GC-MS. Int J Pharmacogn Phytochem Res 2017;9:885-91.
20. Ashraf A, Sarfraz RA, Rashid MA, Mahmood A, Shahid M, Noor N, et al. Chemical composition, antioxidant, antitumor, anticancer and cytotoxic effects of Psidium guajava leaf extracts. Pharm Biol 2016;54:1971-81.
21. Yang D, Michel L, Chaumont JP, Millet-Clerc J. Use of caryophyllene oxide as an antifungal agent in an in vitro experimental model of onychomycosis. Mycopathologia 1999;148:79-82.
22. Prakasia P, Nair A. Chemical fingerprint of essential oil components from fresh leaves of Glycosmis pentaphylla (Retz.) Correa. Pharm Innov J 2015;3:50-6.
23. Durán P, Maria JA, José MB, James RB, Collado B, Isidro Rosario G, et al. Biological activity of natural sesquiterpenoids containing a gem – Dimethylcyclopropane unit. Nat Prod Rep 2012;32:1-3.
24. Ishii N, Senoo-Matsuda N, Miyake K, Yasuda K, Ishii T, Hartman PS, et al. Coenzyme Q10 can prolong C. elegans lifespan by lowering oxidative stress. Mech Ageing Dev 2004;125:41-6.
25. Zhuanhua W, Xiaoli M, Jiao L, Xiaodong C. Peptides from sesame cake extend healthspan of Caenorhabditis elegans, via upregulation of skn-1, and inhibition of intracellular ROS levels. Exp Gerontol 2016;82:139-49.
26. Kumar GP, Khanum F. Neuroprotective potential of phytochemicals. Pharmacogn Rev 2012;6:81-90.
27. Olajuyigbe OO, Afolayan AJ. Phenolic content and antioxidant property of the bark extracts of Ziziphus mucronata willd. Subsp. Mucronata willd. BMC Complement Altern Med 2011;11:130.
28. Arora A, Byrem TM, Nair MG, Strasburg GM. Modulation of liposomal membrane fluidity by flavonoids and isoflavonoids. Arch Biochem Biophys 2000;373:102-9.
29. Fernandes RP, Trindade MA, Tonin FG, Lima CG, Pugine SM, Munekata PE, et al. Evaluation of antioxidant capacity of 13 plant extracts by three different methods: Cluster analyses applied for selection of the natural extracts with higher antioxidant capacity to replace synthetic antioxidant in lamb burgers. J Food Sci Technol 2016;53:451-60.
30. Alia M, Horcajo C, Bravo L, Goya L. Effect of grape antioxidant dietary fiber on the total antioxidant capacity and the activity of liver antioxidant enzymes in rats. Nutr Res 2003;23:1251-67.
31. Rao AS, Reddy SG, Babu PP, Reddy AR. The antioxidant and antiproliferative activities of methanolic extracts from njavara rice bran. BMC Complement Altern Med 2010;10:4.
32. Jagadish LK, Krishnan VV, Shenbhagaraman R, Kaviyarasan V. Comparative study on the antioxidant, anticancer and antimicrobial property of Agaricus bisporus Imbach before and after boiling. Afr J Biotechnol 2009;8:654-61.
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How to Cite
IDOWU JONAS SAGBO, and WILFRED OTANG MBENG. “CHEMICAL CONSTITUENTS, ANTIOXIDATIVE, CYTOTOXIC, AND GENOTOXIC EFFECTS OF MISCANTHUS CAPENSIS ROOTS EXTRACT”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 12, Nov. 2019, pp. 227-32, doi:10.22159/ajpcr.2019.v12i12.35302.
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