IN VITRO STUDIES ON THE ANTIDIABETIC ACTIVITY OF 2-THIOHYDANTOIN USING α-amylase AND α-GLUCOSIDASE

  • UMA S Department of Biotechnology, Dwaraka Doss Goverdhan Doss Vaishnav College, Chennai, Tamil Nadu, India.
  • DEVIKA PT Department of Biochemistry, Mohamed Sathak College of Arts and Science, Tamil Nadu, India.

Abstract

Objective: The aim of the present study was to investigate the inhibitory effect of 2-thiohydantoin on the enzymes α-amylase and α-glucosidase in vitro as an antidiabetic therapeutic approach to reduce gastrointestinal glucose production.


Methods: Antidiabetic activity of the compound, 2-thiohydantoin was measured using the enzymes α-amylase and α-glucosidase.


Results: Half-maximal inhibitory concentration of the antidiabetic α-amylase was found to be 410.35 μg/ml and α-glucosidase was found to be 356.33 μg/ml, respectively.


Conclusion: The result obtained in the in vitro enzyme assay suggests the antidiabetic activity of 2-thiohydantoin.

Keywords: 2-thiohydantoin, α-amylase, α-glucosidase, Antidiabetic activity

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UMA S, and DEVIKA PT. “IN VITRO STUDIES ON THE ANTIDIABETIC ACTIVITY OF 2-THIOHYDANTOIN USING α-Amylase AND α-GLUCOSIDASE”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 12, Oct. 2019, pp. 155-7, doi:10.22159/ajpcr.2019.v12i12.35320.
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