SCREENING AND EVALUATION OF SECONDARY METABOLITES PRESENT IN PIPER CUBEBA
Bioactive compounds like phytochemicals are extracted mainly from plants, as they serve as traditional herbal medicine. The secondary metabolite such as alkaloids, flavonoids, phenolic compounds etc., serve as natural antioxidants and has been widely used because of its therapeutic applications. The objective of the present study was to do preliminary phytochemical screening of the spice Piper cubeba. Out of all the three extracts used, methanolic extract showed higher release of secondary metabolites when compared to ethyl acetate and hexane extract. Further quantitative analysis was carried out with the methanolic extract. Significant result such as 1040 µg of GAE/g of total concentration of flavonoid and were found to be 1280 µg of QE/g of total phenolic content was obtained.
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