EFFECTS OF ETHANOLIC EXTRACT OF RHINACANTHUS NASUTUS (L) KURZ AS AN ANTI MICROBIAL AGAINST ESCHERICHIA COLI BACTERIA USING IN VITRO METHOD
Objective:The objective of this research was to discover the inhibitory efficacy of ethanolic extract of Rhinacanthus nasutus (L) Kurz against Escherichia coli bacteria using in vitro method.
Methods:This is an experimental research using a laboratory test with Kirby Bauer or Paper disk method by observing and measuring the zone of inhibition of ethanolic extractof R. nasutus against Escherichia coli bacteria with extract concentrations of 15%, 30% and 60% consisting of control groups and treatment group. Positive control group used chloramphenicol antibiotics and negative control groups used aquadest. Escherichia coli was incubated at 37oC for 1x24 hours. Then the plates were incubated for 24 hours at 37oC and the diameter of the inhibition zone was observed until the third day with 3 repetitions.
Results:The results of the research showed that the mean of inhibition zone of Escherichia coli bacteria was 10.93 mm; 12.09 mm; and 18.90 mm. The results of the Shapiro-Wilk test was p= 0.199. The results of the One way Anova test was p< 0.05 and that of Post Hoc test indicated significant value of p<0.05. Based on the results of the research there were significant differences in the inhibition zone between the control group and the treatment group at a concentration of 15%, 30% and 60%.
Conclusion:R. nasutus extract was effective to inhibit the growth of Escherichia coli bacteria at concentrations of 15%, 30% and 60%.
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