Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar -125 001 (Haryana)

  • Dinesh Dhingra Dept. of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, HISAR-125001 (Haryana)
  • Sudha Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar -125 001 (Haryana)


Objectives: The present study was undertaken to investigate the antidepressant potential of trans-anethole in unstressed and stressed male mice.

Methods: Swiss albino male mice were exposed to chronic unpredictable mild stress for 21 successive days. Simultaneously, trans-anethole (12.5 mg/kg, 25 mg/kg, 50 mg/kg) and fluoxetine (20 mg/kg) per se were administered for 21 successive days to separate groups of unstressed and stressed mice.  Effect of drugs on depressive-like behavior of mice was tested by tail suspension test and sucrose preference test. 

Results: Trans-anethole (25 mg/kg) and fluoxetine significantly decreased immobility period of unstressed and stressed mice in TST as compared to their respective control. These drugs significantly restored the reduced sucrose preference (%) in stressed mice. Trans-anethole did not show any significant effect on locomotor activity of mice. Antidepressant-like activity of trans-anethole (25 mg/kg) was found to be comparable to fluoxetine. Trans-anethole and fluoxetine significantly inhibited brain MAO-A activity, decreased plasma nitrite, brain malondialdehyde; and increased brain reduced glutathione levels and catalase activity in unstressed and stressed mice. The drugs significantly reversed stress-induced increase in plasma corticosterone levels.

Conclusion: Trans-anethole produced significant antidepressant-like activity in unstressed and stressed mice possibly through inhibition of brain MAO-A activity and alleviation of oxidative stress. Reversal of stress- induced increase in plasma corticosterone levels might also be responsible for antidepressant-like activity of trans-anethole in stressed mice.

Keywords: Antidepressant, Chronic unpredictable mild stress, Depression, Trans-anethole, Tail suspension test.


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How to Cite
Dhingra, D., and Sudha. “ANTIDEPRESSANT- LIKE ACTIVITY OF TRANS-ANETHOLE IN UNSTRESSED MICE AND STRESSED MICE”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 12, Oct. 2019,
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