AMELIORATION OF ACETAMINOPHEN-INDUCED HEPATOTOXICITY USING SOLANUM TORVUM SW. FRUIT EXTRACT IN WISTAR RATS

  • UMERA BEGAM AK Department of Biochemistry, Holy Cross College (Affiliated to Bharathidasan University), Tiruchirappalli, Tamil Nadu, India.
  • SENTHILKUMAR R Department of Biochemistry, Periyar E.V.R. College (Affiliated to Bharathidasan University), Tiruchirappalli, Tamil Nadu, India.
  • SIRAJUDEEN J Department of Chemistry, Jamal Mohammed College (Affiliated to Bharathidasan University), Tiruchirappalli, Tamil Nadu, India.

Abstract

Objective: Ayurveda and Chinese pharmacopeia have highlighted the traditional medicinal uses of Solanum torvum Sw. The fruits are ethnomedical used in the treatment of liver and spleen enlargement, cough, and also used as a hematopoietic, antimicrobial, and analgesic agent. In the present study, the amelioration of acetaminophen (APAP)-induced hepatotoxicity of the aqueous extract of S. torvum Sw. fruits is evaluated.


Methods: The hepatoprotective activity of the fruit extract against APAP insult was evaluated by assessing it is in vivo antioxidants status, membrane-bound adenosine triphosphatases (ATPases), and tricarboxylic acid (TCA) cycle marker enzymes and also through histopathological studies of the liver.


Results: Administration of the aqueous fruit extract of the plant caused a significant increase in the in vivo antioxidant status as evident from the reduction in lipid peroxidation caused by APAP and improvement in the mitochondrial membrane stability which is proved from the activity of membrane-bound ATPases and TCA cycle marker enzymes. Histological studies also supported the fact that the plant extract proved to revive the architecture of the toxin damaged liver tissues in par with silymarin. The chemical pathological changes were consistent with histopathological observations suggesting marked hepatoprotective effect of the aqueous extract of S. torvum.


Conclusion: The results showed that the extract of S. torvum Sw. fruits has hepatoprotective potential which may be due to the antioxidant activity of its phytoconstituents, especially flavonoids, alkaloids, phenolics, etc.

Keywords: Hepatoprotective, Liver, Paracetamol, Acetaminophen, Solanum torvum, Antioxidant, Adenosine triphosphatases, Tricarboxylic acid marker enzymes

References

1. Bernal W, Donaldson N, Wyncoll D, Wendon J. Blood lactate as an early predictor of outcome in paracetamol-induced acute liver failure: A cohort study. Lancet 2002;359:558-63.
2. Moukette BM, Pieme CA, Njimou JR, Biapa CP, Marco B, Ngogang JY. In vitro antioxidant properties, free radicals scavenging activities of extracts and polyphenol composition of a non-timber forest product used as spice: Monodora myristica. Biol Res 2015;48:15.
3. Nguelefacka TB, Catherine BF, Gilber A, Pierre W, Simplice T, Albert DA, et al. Anti-ulcerogenic properties of the aqueous and methanol extracts from the leaves of Solanum torvum Swartz (Solanaceae) in rats. J Ethnopharmacol 2008;119:135-40.
4. Deepak K, Nayak G, Rajalingam D, Sengupta P, Maity K. Evaluation of hepatoprotective and antioxidant activity of Ichnocarpus frutescens (Linn.) R.Br. On paracetamol induced hepatotoxicity in rats. Trop J Pharm Res 2007;6:755-65.
5. Sun F, Hamagawa E, Tsutsui C, Sakaguchi N, Kakuta Y, Tokumaru S, et al. Evaluation of oxidative stress during apoptosis and necrosis caused by D-galactosamine in rat liver. Biochem Pharmacol 2003;65:101-7.
6. Abirami A, Nagarani G, Siddhuraju P. Hepatoprotective effect of leaf extracts from Citrus hystrix and C. Maxima against paracetamol induced liver injury in rats. Food Sci Hum Wellness 2015;4:35-41.
7. Zoubair B, Azzahra LF, Fouzia H, Mohammed L, Brahim B, Noureddine B. Evaluation of acetaminophen effect on oxidative stressed mice by peroxide hydrogen. Am J Biomed Res 2013;1:75-9.
8. Asadi-Samani M, Kafash-Farkhad N, Azimi N, Fasihi A, Alinia- Ahandani E, Rafieian-Kopa M. Medicinal plants with hepatoprotective activity in Iranian folk medicine. Asian Pac J Trop Biomed 2015;5:146-57.
9. Younes M, Siegers CP. The role of iron in the paracetamol and CCl4- induced lipid peroxidation and hepatotoxicity. Chem Biol Interact 1985;55:327-34.
10. Haraguchi H, Yosh, Jhlkawa H. ???. J Pharm Pharmacol 2000;52:315.
11. Papadimitrious JC, Phelps PC, Shin ML, Smith MW, Trump BF. Effects of Ca2+ deregulation on mitochondrial membrane potential and cell viability in nucleated cells following lytic complement attack. Cell Calcium 1994;15:217-27.
12. Hebbel RP, Shalev O, Foker W, Rank BH. Inhibition of erythrocyte Ca2+-ATPase by activated oxygen through thiol-and lipid-dependant mechanisms. Biochem Biophys Acta 1986;862:8-16.
13. Al-Numair KS, Chandramohan G, Alsaif MA, Veeramani C, El Newehy AS. Morin, a flavonoid, on lipid peroxidation and antioxidant status in experimental myocardial ischemic rats. Afr J Tradit Complement Altern Med 2014;11:14-20.
14. de Hingh YC, Meyer J, Fischer JC, Berger R, Smeitink JA, Op den kamp JA. Direct measurement of lipid peroxidation in submitochondrial particles. Biochemistry 1995;34:12755-60.
15. Capetenaki Y. Desmin cytoskeleton: A potential regulator of muscle mitochondria behavior and function. Trends Cardiovasc Med 2008;12:339-48.
16. Arathi G, Sachdanandam P. Therapeutic effect of Semecarpus anacardium Linn. Nut milk extract on carbohydrate metabolizing and mitochondrial TCA cycle and respiratory chain enzymes in mammary carcinoma rats. J Pharm Pharmacol 2003;55:1283-90.
17. Sohal RS, Weindruch R. Oxidative stress calorie restriction and aging. Science 1996;273:59-63.
18. Yogi B, Mishra A. Hepatoprotective effects of polyherbal formulation against carbon tetrachloride-induced hepatic injury in albino rats: A toxicity screening approach. Asian J Pharm Clin Res 2017;10:192-5.
19. Saha P, Talukdar AD, DuttaChoudhury M. Antioxidant potential profile of Pajanelia longifolia (willd.) K. Schuman; potential new sources of natural antioxidant. Asian J Pharm Clin Res 2017;10:184-8.
Statistics
69 Views | 11 Downloads
Citatons
How to Cite
UMERA BEGAM AK, SENTHILKUMAR R, and SIRAJUDEEN J. “AMELIORATION OF ACETAMINOPHEN-INDUCED HEPATOTOXICITY USING SOLANUM TORVUM SW. FRUIT EXTRACT IN WISTAR RATS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 1, Dec. 2019, pp. 238-42, doi:10.22159/ajpcr.2020.v13i1.35564.
Section
Original Article(s)