PHYTOCHEMICAL SCREENING, GAS CHROMATOGRAPHY–MASS SPECTROMETRY PROFILING, IN VITRO CYTOTOXICITY, AND ANTHELMINTIC ACTIVITY OF HYDROALCOHOLIC EXTRACT OF DURANTA ERECTA LEAVES

  • RAJESWARI R Department of Pharmacology, The Tamilnadu Dr. MGR. Medical University, Chennai, Tamil Nadu, India.
  • PRABHAKARAN V Department of Pharmacology, The Tamilnadu Dr. MGR. Medical University, Chennai, Tamil Nadu, India.
  • SARAVANAKUMAR A Department of Pharmacology, The Tamilnadu Dr. MGR. Medical University, Chennai, Tamil Nadu, India.

Abstract

Objective: The present study aims at the preliminary phytochemical evaluation, gas chromatography–mass spectrometry (GC–MS) analysis, in vitro cytotoxicity, and anthelmintic activity of the hydroalcoholic extract of D. erecta leaves.


Methods: The hydroalcoholic extraction of the plant material is done with Soxhlet apparatus and the GC–MS profiling of the extract is performed. The in vitro cytotoxicity activity of the extract is done using the MOLT-3 cell lines. The anthelmintic activity is performed using Pheretima posthuma.


Results: The GC–MS analysis shows the presence of various secondary metabolites with proved pharmacological activity. The in vitro cytotoxicity and the anthelmintic activity of the extract show the beneficial effect of the plant.


Conclusion: The flavonoids, phenolic compounds, and tannins present in the plant extract may support the cytotoxic and anthelmintic activity of the plant.

Keywords: Gas chromatography–mass spectrometry, MOLT, Cytotoxicity, Anthelmintic

Author Biography

RAJESWARI R, Department of Pharmacology, The Tamilnadu Dr. MGR. Medical University, Chennai, Tamil Nadu, India.

Asst Professor Department of Pharmacology

References

1. Duraiswamy B, Singanan M, Varadarajan V. Physicochemical, phytochemicals and antioxidant evaluation of Guazuma ulmifolia fruit. Int J Pharm Pharm Sci 2018;10 Suppl 9:87. Available from: https://www.innovareacademics.in/journals/index.php/ijpps/article/view/26778.
2. Sahil K, Prashant B, Akanksha M, Premjeet S, Devashish R. GC-MS: Applications. Int J Pharma Biol Arch 2011;2:1544-60.
3. Krone N, Hughes BA, Lavery GG, Stewart PM, Arlt W, Shackleton CH. Gas chromatography/mass spectrometry (GC/MS) remains a pre-eminent discovery tool in clinical steroid investigations even in the era of fast liquid chromatography tandem mass spectrometry (LC/MS/MS). J Steroid Biochem Mol Biol 2010;121:496-504.
4. Vijayarathna S, Sasidharan S. Cytotoxicity of methanol extracts of Elaeis guineensis on MCF-7 and vero cell lines. Asian Pac J Trop Biomed 2012;2:826-9.
5. Omale J, Ojonugwa T. In vitro antihelmintic activity of Saba Florida (benth) extracts against Nigerian adult earth worm (Terrestris lumbricoides). Planta Med 2014;80 Suppl 16:P1L77.
6. Prashant T, Bimlesh K, Mandeep K, Gurpreet K, Harleen K. Phytochemical screening and extraction: A review. Int Pharm Sciencia 2011;1:98-106.
7. Harborne JB. Phytochemical Methods. London; Chapman and Hall, Ltd.; 1973. p. 49-188.
8. Vogel AI. Text Book of Practical Organic Chemistry. London: The English Language Book Society and Longman; 1978. p. 1368.
9. Dhivya R, Manimegalai K. Preliminary phytochemical screening and GC-MS profiling of ethanolic flower extract of Calotropis gigantea Linn. (Apocynaceae). J Pharmacogn Phytochem 2013;2 Suppl 3:28-32. Available from: https://www.pdfs.semanticscholar.org/ea63/10ca7adbe3164b4744f5a761edb1142d0ca2.pdf?_ga=2.27559740.1824053717.1546633376-376688916.1546633376.
10. Delcastillo J, Demello WC, Morales T. Mechanism of the paralysing action of piperazine on Ascaris muscle. Br J Pharmacol Chemother 1964;22 Suppl 3:463-77.
11. Aziz A, Sarwar Raju G, Das A, Ahmed J, Moghal MM. Evaluation of in vitro anthelmintic activity, total phenolic content and cytotoxic activity of Crinum latifolium L. (Family: Amaryllidaceae). Adv Pharm Bull 2014;4:15-9.
12. Tiwari P, Kumar B, Kumar M, Kaur M, Debnath J, Sharma P. Comparative anthelmintic activity of aqueous and ethanolic stem extract of Tinospora cordifolia. Int J Drug Dev Res 2011;3 Suppl 1:70-83.
13. Dewan SM, Das A. Investigation of in vitro thrombolytic potential and phytochemical nature of Crinum latifolium L. leaves growing in coastal region of Bangladesh. Int J Biol Pharm Res 2013;4 Suppl 1:1-7.
14. Gulilat H, Sahabjada S, Jafri A, Arshad M, Malik T. The phytochemical investigation, GC-MS profile and antimicrobial activity of a medicinal plant Ruta graveolens L. from Ethiopia. Int J Pharm Pharm Sci 2017;9 Suppl 6:29-34.
15. Antoney J, Britto AJ, Abida P, Stephan TL. In vitro cytotoxicity studies on methanolic leaf extract of Embelia Ribes Burm F an important traditional medicinal plant of Kerala. Adv Cytol Pathol 2016;1 Suppl 1:2.
16. Mioc M, Pavel IZ, Ghiulai R, Coricovac DE, Farca? C, Mihali CV, et al. The cytotoxic effects of betulin-conjugated gold nanoparticles as stable formulations in normal and melanoma cells. Front Pharmacol 2018;9:429.
17. Pretorius E, Oberholzer HM, Becker PJ. Comparing the cytotoxic potential of Withania somnifera water and methanol extracts. Afr J Tradit Complement Altern Med 2009;6:275-80.
18. Fulda S. Betulinic acid for cancer treatment and prevention. Int J Mol Sci 2008;9:1096-107.
19. Zhao J, Li R, Pawlak A, Henklewska M, Sysak A, Wen L, et al. Antitumor activity of betulinic acid and betulin in canine cancer cell lines. In Vivo 2018;32:1081-8.
20. Lee IC, Choi BY. Withaferin-A--A natural anticancer agent with pleitropic mechanisms of action. Int J Mol Sci 2016;17:290.
21. Król SK, Kielbus M, Rivero-Müller A, Stepulak A. Comprehensive review on betulin as a potent anticancer agent. Biomed Res Int 2015;2015:584189.
22. Kalmobé J, Ndjonka D, Boursou D, Vildina JD, Liebau E. Phytochemical analysis and in vitro anthelmintic activity of Lophira lanceolata (Ochnaceae) on the bovine parasite Onchocerca ochengi and on drug resistant strains of the free-living nematode Caenorhabditis elegans. BMC Complement Altern Med 2017;17 Suppl 1:404.
23. Mute VM. Anthelmintic effect of Tamarind indica Linn leaves juice exract on Pheretima posthuma. Int J Pharm Res Dev 2009;7:1-6.
24. Swargiary A, Daimari A, Daimari M, Basumatary N, Narzary E. Phytochemicals, antioxidant, and anthelmintic activity of selected traditional wild edible plants of lower Assam. Indian J Pharmacol 2016;48 Suppl 4:418-23.
25. Yu Y, Katiyar SP, Sundar D, Kaul Z, Miyako E, Zhang Z, et al. Withaferin-A kills cancer cells with and without telomerase: Chemical, computational and experimental evidences. Cell Death Dis 2017;8:e2755.
26. Sasi S, Anjum N, Tripathi YC. Ethnomedicinal, phytochemical and pharmacological aspects of Flacourtia jangomas: A review. Int J Pharm Pharm Sci 2018;10 Suppl 3:9-15. Available from: https://www.innovareacademics.in/journals/index.php/ijpps/article/view/23998.
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R, R., PRABHAKARAN V, and SARAVANAKUMAR A. “PHYTOCHEMICAL SCREENING, GAS CHROMATOGRAPHY–MASS SPECTROMETRY PROFILING, IN VITRO CYTOTOXICITY, AND ANTHELMINTIC ACTIVITY OF HYDROALCOHOLIC EXTRACT OF DURANTA ERECTA LEAVES”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 1, Nov. 2019, pp. 63-68, doi:10.22159/ajpcr.2020.v13i1.35592.
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