ISOLATION OF UROPATHOGENS AND THEIR ANTIBIOTIC SUSCEPTIBILITY PATTERN AT A TERTIARY CARE HOSPITAL IN NORTHERN INDIA.

  • Harit Kumar Tutor, Department of Microbiology, MMIMSR, Maharishi Markandeshwar (Deemed to be University)
  • Varsha A Singh Professor and Head, Department of Microbiology, MMIMSR, MMDU
  • Shavi Nagpal Assistant Professor, Department of Microbiology, MMIMSR, Maharishi Markandeshwar (Deemed to be University)
  • Dipankar Biswas Tutor, Department of Microbiology, MMIMSR, Maharishi Markandeshwar (Deemed to be University), Mullana

Abstract

ABSTRACT


Objective:  To know the isolation rate and antibiotic susceptibility pattern of pathogens causing urinary tract infection(UTI).


Methods: A total of 500 urine samples of clinically suspected urinary tract infection were collected from January, 2019 to June 2019. The samples were inoculated on cystine lactose electrolyte deficient (CLED) agar and incubated at 37°C for 24 hrs.


Results: In our study, 303 (60.6%) samples showed growth of isolates. Among 303 isolates, 31 were Candida spp. which was not included in study. Out of 272 isolates, Escherichia coli was 31.68%, followed by Klebsiella spp. (27.72%), Enterococcus faecalis (22.44%), Citrobacter spp. (3.96%), Staphylococcus aureus (2.64%), Pseudomonas spp. (1.32%). For Gram-negative and gram-positive isolates the most effective antibiotic was nitrofurantoin.


Conclusion: The study shows Escherichia coli as the predominant pathogen that causes UTI. nitrofurantoin and fosfomycin showed very high susceptibility to uropathogens and can be used to treat primary or uncomplicated UTI.


Keywords: Urinary tract infection, Escherichia coli, Klebsiella spp., Enterococcus faecalis Nitrofurantoin, Fosfomycin.

Keywords: Urinary tract infection, Escherichia coli, Klebsiella spp., Enterococcus faecalis Nitrofurantoin, Fosfomycin

References

1. Patel HB, Soni ST, Bhagyalaxmi A, Patel NM. Causative agents of urinary tract infections and their antimicrobial susceptibility patterns at a referral center in Western India: An audit to help clinicians prevent antibiotic misuse. J Family Med Prim Care. 2019;8:154-9.
2. Kumar et al. Uropathogens: isolation and antibacterial susceptibility pattern. Asian J Pharm Clin Res. 2017;10(6):222-224.
3. Muraleetharan M, Viswanathan T. Epidemiological studies on varying extended-spectrum ?-lactamases producing uropathogenic bacteria. Int J Pharm Pharm Sci 2014;6(11):57-60.
4. Foxman B. Epidemiology of urinary tract infections: Incidence, morbidity, and economic costs. Dis Mon 2003;49(2):53-70.
5. Mollick S, Dasgupta T, Hasnain MJ, Ahmed M. Isolation and characterization of pathogens responsible for urinary tract infection in Bangladesh and determination of their antibiotic susceptibility pattern. J App Pharm Sci 2016;6(04):72-6.
6. Chaudhary BL, Insan NG, Payal N, Vashistha R, Rajput A. ESBL and AmpC detection in Klebsiella species by non-molecular methods. Int J Curr Microbiol App Sci 2014;3(4):323-9.
7. Spoorenberg V, Hulscher ME, Akkermans RP, Prins JM, Geerlings SE. Appropriate antibiotic use for patients with urinary tract infections reduces length of hospital stay. Clin Infect Dis 2014;58:164?9.
8. Kass EH. Bacteriuria and the diagnosis of infections of the urinary tract; with observations on the use of methionine as a urinary antiseptic. AMA Arch Intern Med 1957;100:709?14.
9. Collee JG, Miles RS, Watt B. Test for identification of bacteria. In: Collee JG, Fraser AG, Marmion BP, Simmons A, editors. Mackie and McCartney Practical Medical Microbiology. 14th ed. London: Churchill Livingstone Inc.; 1996. p. 435
10. CLSI. Performance Standards for Antimicrobial Susceptibility Testing. 29th ed. CLSI Supplement M100. Wayne, PA: Clinical and Laboratory Standards Institute; 2019.
11. Maji SK, Mandal PK, Panja C, Dolai TC, Samanta A, Kundu PK, et al. Prevalence and antibacterial susceptibility pattern of aerobic bacteria causing urinary tract infection in tribal population in rural part of West Bengal, India. Int J Curr Microbiol App Sci 2016;5(6):406-12.
12. Basavaraj MB, Jyothi P. Antibiotic sensitivity pattern of Citrobacter spp. Isolated from patients with urinary tract infections in tertiary care hospital in south India. Int J Pharm Pharm Sci 2015;7(1):252-4.
13. Lawhale MA, Naikwade R. Recent pattern of drug sensitivity of most commonly isolated uropathogens from Central India. Int J Res Med Sci 2017;5:3631-6.
14. Dipak B, Raj PB, Amarullah S et al. Bacterial Uropathogens and their Antibiotic Sensitivity Pattern at a Tertiary Care Hospital in Birgunj, Nepal. MED PHOENIX : An Official Journal of NMC, Birgunj, Nepal 2017;2(1) :74-77.
15. Biswas R, Rabbani R, Ahmed HS et al. Antibiotic sensitivity pattern of urinary tract infection at a tertiary care hospital. Bangladesh Crit Care J March 2014; 2 (1): 21-24
16. George CE, Norman G, Ramana GV, Mukherjee D, Rao T. Treatment of uncomplicated symptomatic urinary tract infections: Resistance patterns and misuse of antibiotics. J Family Med Prim Care 2015;4:416?21.
Statistics
11 Views | Downloads
How to Cite
Kumar, H., V. A. Singh, S. Nagpal, and D. Biswas. “ISOLATION OF UROPATHOGENS AND THEIR ANTIBIOTIC SUSCEPTIBILITY PATTERN AT A TERTIARY CARE HOSPITAL IN NORTHERN INDIA.”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 12, Oct. 2019, https://innovareacademics.in/journals/index.php/ajpcr/article/view/35692.
Section
Original Article(s)