IN VITRO ANALYSIS OF ANTICANCER POTENTIAL OF TRIGONELLA FOENUM-GRAECUM

  • SHIVSHARAN SINGH Department of Life Science, Uttarakhand Technical University, Suddhowala, Dehradun, Uttarakhand, India.
  • SATISH K VERMA Department of Life Science, Kishori Lal (PG) College, Naini, Allahabad, Uttar Pradesh, India.
  • SANTOSH SINGH Department of Life Science, Beehive Group of Colleges, Selaqui, Chakrata Road Dehradun, Uttarakhand, India.

Abstract

Objective: The recent work was carried out to evaluate the presence of bioactive compounds and anticancer activities of selected plant Trigonella foenum-graecum extract.


Methods: The crude extraction of whole plant was carried out using petroleum ether as a solvent by cold percolation as well as hot percolation method (Soxhlet method) both. The presence of phytochemicals was determined using standard protocols. The anticancer activities were evaluated by sulforhodamine B (SRB) dye assay method using Mitomycin-C (anticancer drug) as a positive control.


Results: The qualitative analysis of plant extract showed the presence of bioactive compounds, namely, protein, alkaloids, steroids, phenolics, and flavonoids. The plant extract was tested for in vitro anticancer activity against human liver cancer (HEP-2) and colon cancer (HT-29) cell lines using SRB assay. The plant extract showed significant results against HT-29 and HEP-2 cancer cell lines.


Conclusion: The study concluded that the extract of T. foenum-graecum can be further carefully used in herbal formulations for cancer therapy.

Keywords: Synthetic, Anticancer, Trigonella foenum-graecum, Herbal, Flavonoids

References

1. American Cancer Society. A Biotechnology Company Dedicated to Cancer Treatment on Jan 26. Atlanta, Georgia: American Cancer Society; 2006. Available from: http://www.cancervax.com/info/index.htm.
2. Ahmad I, Mehmood Z, Mohammad F. Screening of some Indian medicinal plants for their antimicrobial properties. J Ethnopharmacol 1998;62:183-93.
3. Ankli A, Heinrich M, Bork P, Wolfram L, Bauerfeind P, Brun R, et al. Yucatec Mayan medicinal plants: Evaluation based on indigenous uses. J Ethnopharmacol 2002;79:43-52.
4. Datta BK, Rahman I, Das TK. Antifungal activity of Indian plant extracts. Mycoses 1998;41:535-536.
5. Neto CC. Antibacterial activity of some Peruvian medicinal plants from the Callejon de Huaylas. J Ethnopharmacol 2002;79:133-8.
6. Shanmugasundaram S. Complementary and alternative therapies in palliative care: A transition from modern medicine to traditional medicine in India. J Cancer Pain Symptom Palliation 2005;1:25-9.
7. Das AM, Dhanabalan R, Palaniswamy M. Phytochemical screening and antibacterial activity of aqueous and methanolic leaf extracts of two medicinal plants against bovine mastitis bacterial pathogens. Ethnobot Leaf 2009;13:131-9.
8. Hartwell JL. Plants used against Cancer. A Survey. Lawrence, MA: Quarterman Publications; 1982.
9. Pandey M, Debnath M, Gupta S, Chikara, SK. Phytomedicine: An ancient approach turning into future potential source of therapeutics. J Pharmacogn Phytother 2011;3:27-37.
10. Denny B, Wansbrough H. The design and development of anti-cancer drugs. Biotech J Cancer Drugs 1995;12:1-12.
11. Rao NJ, Subash KR, Kumar KS. Role of phytotherapy in gingivitis, a review. Int J Pharmacol 2002;8:1-5.
12. Prachayasittikul S, Saraban P, Cherdtrakulkiat R, Ruchirawat S, Prachayasittikul V. New bioactive triterpenoids and antimalarial activity of Diospyros rubra Lec. EXCLI J 2010a;9:1-10.
13. Prachayasittikul V. Antimicrobial and antioxidative activities of bioactive constituents from Hydnophytum formicarum Jack. Molecules 2008;13:904-21.
14. Tiwari KL, Jadhav SK, Joshi V. An updated review on medicinal herb genus Spilanthes. Chin J Integr Med 2011;9:1170-8.
15. Preeti K, Namdeo J. Phytochemical screening and in-vitro anticancer activity of extracts of Tectaria cicutaria. Int J Pharm Sci Res 2018;9:3463-8.
16. Petropoulos GA. Fenugreek the Genus Trigonella. 1st ed. London, New York: Taylor and Francis; 2002. p. 1-127.
17. Basch E, Ulbricht C, Kuo G, Szapary PH, Smith M. Therapeutic applications of Fenugreek. Altern Med Rev 2003;8:20-7.
18. Toppo FA, Akhand R, Pathak AK. Pharmacological actions and potential uses of Trigonella foenum-graecum: A review. Asian J Pharm Clin Res 2009;2:29-38.
19. Trease GE, Evans WC. Pharmacognosy. 15th ed. New York: WB Saunders; 2002. p. 214-393.
20. Verma SK, Singh SK, Singh SS, Mathur A. In vitro cytotoxicity of Cannabis sativa and Trigonella foenum graecum against human cancer cell lines. J Chem Pharm Res 2010;2:861-5.
21. Monks A. Feasibility of a high-flux anticancer drug screen using a diverse panel of cultured human tumor cell lines. J Natl Cancer Inst 1991;83:757-66.
22. Skehan P. New colorimetric cytotoxicity assay for anticancer drug screening. J Natl Cancer Inst 1990;82:1107-12.
23. Vichai V, Kirtikara K. Sulforhodamine B colorimetric assay for cytotoxicity screening. Nature Protoc 2006;1:1112-6.
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SINGH, S., SATISH K VERMA, and SANTOSH SINGH. “IN VITRO ANALYSIS OF ANTICANCER POTENTIAL OF TRIGONELLA FOENUM-GRAECUM”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 1, Nov. 2019, pp. 189-92, doi:10.22159/ajpcr.2020.v13i1.36010.
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