BIOSYNTHESIS OF COPPER NANOPARTICLES USING PARTHENIUM HYSTEROPHORUS LEAF EXTRACT AND SCREENING ITS ANTIMICROBIAL ACTIVITY

  • ROHINI SAHAYA MARY Department of Botany, Teresian College, Mysore, Karnataka, India.
  • MAHESH MK Department of Botany, Yuvaraja’s College, Mysore, Karnataka, India.
  • PRUTHVI ML Department of Botany, Yuvaraja’s College, Mysore, Karnataka, India.

Abstract

Objective: The aim of this study is to synthesis the copper nanoparticles (CuNPs) using the leaf extract of Parthenium hysterophorus.


Methods: Dry and fresh leaf extract was prepared and CuSo4 was added. The color change was noted and recorded by ultraviolet–visible spectrophotometer. The morphological characteristics were analyzed by scanning electron microscopy (SEM). Antimicrobial activities were performed by the disc diffusion method.


Results: The color change indicates the production of CuNPs. Surface plasmon resonance band was observed around 599 nm and 572 nm for fresh and dry samples of P. hysterophorus leaf extract. SEM confirms the formation and the crystalline nature of CuNPs and X-ray diffraction studies show the particle size. The antibacterial potentials of the CuNPs were studied and have shown good high inhibition activity against Staphylococcus aureus, Bacillus subtilis, Proteus vulgaris, and Pseudomonas aeruginosa at different concentrations in compare to fungi species.


Conclusion: This method is effective and environmental friendly for the synthesis of CuNPs using leaf extract of Parthenium hysterophorus.

Keywords: Copper nanoparticles, Parthenium hysterophorus, Antimicrobial activity, Scanning electron microscopy, X-ray diffraction

References

1. Shankar SS, Rai A, Ahmad A, Sastry M. Rapid synthesis of Au, Ag, and bimetallic Au core-Ag shell nanoparticles using neem (Azadirachta indica) leaf broth. J Colloid Interface Sci 2004;275:496-502.
2. Kumar V, Yadav SK. Plant-mediated synthesis of silver and gold nanoparticles and their applications. J Chem Technol Biotechnol 2009;84:151-7.
3. Mukunthan K, Balaji S. Cashew apple juice (Anacardium occidentale L.) speeds up the synthesis of silver nanoparticles. Int J Green Nanotechnol 2012;4:71-9.
4. Sahoo SK, Parveen S, Panda JJ. The present and future of nanotechnology in human health care. Nanomedicine 2007;3:20-31.
5. Mohanraj Y, Chen A. Nanoparticles a review. Trop J Pharm Res 2007;5:561-73.
6. Panyam J, Labhasetwar V. Biodegradable nanoparticles for drug and gene delivery to cells and tissue. Adv Drug Deliv Rev 2003;55:329-47.
7. Han S, Liu Y, Chang A. Cytoplasmic Hsp70 promotes ubiquitination for endoplasmic reticulum-associated degradation of a misfolded mutant of the yeast plasma membrane ATPase, PMA1. J Biol Chem 2007;282:26140-9.
8. Perelshtein I, Applerot G, Perkas N, Wehrschuetz-Sigl E, Hasmann A, Guebitz G, et al. CuO-cotton nanocomposite: Formation, morphology, and antibacterial activity. Surf Coat Technol 2009;204:54-7.
9. Valodkar M. Synthesis and anti-bacterial activity of Cu, Ag and Cu-Ag alloy nanoparticles: A green approach. Mater Res Bull 2011;46:384-9.
10. Kattumuri V, Katti K, Bhaskaran S, Boote EJ, Casteel SW, Fent GM,et al. Gum Arabic as a phytochemical construct for the stabilization of gold nanoparticles: In vivo pharmacokinetics and X-ray-contrast-imaging studies. Small 2007;3:333-41.
11. Virkutyte J, Varma RS. Green synthesis of metal nanoparticles: Biodegradable polymers and enzymes in stabilization and surface functionalization. J Chem Sci 2011;2:837.
12. Wagner MC, Molnar EE, Molitoris BA, Goebl MG. Loss of the homotypic fusion and vacuole protein sorting or golgi-associated retrograde protein vesicle tethering complexes results in gentamicin sensitivity in the yeast Saccharomyces cerevisiae. Antimicrob Agents Chemother 2006;50:587-95.
13. Huang K, Ferrin-O’Connell I, Zhang W, Leonard GA, O’Shea EK, Quiocho FA. Structure of the Pho85-Pho80 CDK-cyclin complex of the phosphate-responsive signal transduction pathway. Mol Cell 2007;28:614-23.
14. Mary S, Mahesh MK. Determination of antibacterial and antifungal activities of endemic tree fern Cyathea nilgirensis plant extracts. Int J Curr Res 2015;7:18276-9.
15. Yoon KY, Hoon Byeon J, Park JH, Hwang J. Susceptibility constants of Escherichia coli and Bacillus subtilis to silver and copper nanoparticles. Sci Total Environ 2007;373:572-5.
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ROHINI SAHAYA MARY, MAHESH MK, and PRUTHVI ML. “BIOSYNTHESIS OF COPPER NANOPARTICLES USING PARTHENIUM HYSTEROPHORUS LEAF EXTRACT AND SCREENING ITS ANTIMICROBIAL ACTIVITY”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 1, Nov. 2019, pp. 93-99, doi:10.22159/ajpcr.2020.v13i1.36076.
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