IN VITRO ANTIMICROBIAL AND ANTI-INFLAMMATORY ACTIVITY OF METHANOL EXTRACT OF ERANTHEMUM CAPENSE
Objective: The present study was aimed to rationalize the scientific basis in traditional use of Eranthemum capense as an antibacterial, antifungal, and anti-inflammatory agent.
Methods: Agar well diffusion method is widely used to evaluate the antimicrobial activity of the E. capense aerial part of methanolic and ethyl acetate plant extracts. The same amount (15–20 mL) of Mueller-Hinton agar was poured on glass Petri plates of same size and allowed to solidify. E. capense aerial part of methanolic and ethyl acetate extracts was evaluated in vitro for their anti-inflammatory activities using the bovine serum albumin protein denaturation assay.
Results: The result of the study shows that methanolic exract (T3) of the plant, E. capense shows 16 mm zone of inhibition against Pseudomonas fluorescens, while the ethyl acetate extract of the same plant shows 14 mm zone of inhibition against P. fluorescens and E. coli. Hence the methanolic extract of T3 sample shows the antibacterial activity against gram negative bacteria, where as the ethyl acetate extract of T3 shows antibacterial activity against both gram positive and gram negative bacteria. The experimental report revealed that, the methanolic and ethyl acetate extract of the same plant produces zero percentage zone of inhibition against Aspergillus niger and Mucor, hence it does not show any antifungal activity.
Conclusion: It is observed that the EA and methanolic extract of E. capense can be used in the treatment of inflammation due to the significant percentage of inhibition of protein denaturation as well as its prove the good antimicrobial agent.
2. Beidokhti MN, Prakash HS. Antioxidant and anti-inflammatory potential of selected medicinal plants of Lamiaceae family. Int J Pharm Pharm Sci 2013;5 Suppl 1:100-4.
3. Mathieu P, Lemieux I, Després JP. Obesity, inflammation, and cardiovascular risk. Clin Pharmacol Ther 2010;87:407-16.
4. Ross R. Atherosclerosis an inflammatory disease. N Engl J Med 1999;340:115-26.
5. Medzhitov R. Inflammation 2010: New adventures of an old flame. Cell 2010;140:771-6.
6. Leyendecker G, Wildt L, Mall G. The pathophysiology of endometriosis and adenomyosis: Tissue injury and repair. Arch Gynecol Obstet 2009;280:529-38.
7. Ferrero-Miliani L, Nielsen OH, Andersen PS, Girardin SE. Chronic inflammation: Importance of NOD2 and NALP3 in interleukin-1beta generation. Clin Exp Immunol 2007;147:227-35.
8. Baron R. Mechanisms of disease: Neuropathic pain a clinical perspective. Nat Rev Neurol 2006;2:95.
9. DeGrado WF, Wasserman ZR, Lear JD. Protein design, a minimalist approach. Science 1989;243:622-8.
10. Chandra S, Chatterjee P, Dey P, Bhattacharya S. Evaluation of in vitro anti-inflammatory activity of coffee against the denaturation of protein. Asian Pac J Trop Biomed 2012;2:S178-80.
11. Chatterjee P, Chandra S, Dey P, Bhattacharya S. Evaluation of anti-inflammatory effects of green tea and black tea: A comparative in vitro study. J Adv Pharm Technol Res 2012;3:136.
12. Santhosh JU, Krishna MJ, Semotiuk AJ, Krishna V. Indigenous knowledge on medicinal plants used by ethnic communities of South India. Ethnobot Res Appl 2019;18:1-12.
13. Chandrashekara UM, Thasini VM. Non-crop edible plants and medicinal plants in homegarden agroforestry system of Palakkad district, Kerala. Int J Ecol Environ Sci 2016;42:183-91.
14. Iswariya S, Uma TS. Evaluation of in vitro anti-inflammatory and antimicrobial activity of aqueous and methanolic seed extracts of Citrullus lanatus. Int J Pharm Pharm Sci 2017;9:29-33.
15. Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: A review. J Pharm Anal 2016;6:71-9.
16. Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999;12:564-82.
17. Stepanovi? S, Anti? N, Daki? I, Švabi?-Vlahovi? M. In vitro antimicrobial activity of propolis and synergism between propolis and antimicrobial drugs. Microbiol Res 2003;158:353-7.
18. Buwa LV, van Staden J. Antibacterial and antifungal activity of traditional medicinal plants used against venereal diseases in South Africa. J Ethnopharmacol 2006;103:139-42.
19. Grant NH, Alburn HE, Kryzanauskas C. Stabilization of serum albumin by anti-inflammatory drugs. Bioche Pharmacol 1970;19:715-22.
20. Krishnananda KK, Ramakrishna S. Comparison of antibacterial activity of leaves extracts of Tectona grandis, Mangifera indica, and Anacardium occidentale. Int J Curr Pharm Res 2017;9:36-9.
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