• SHITAL VALMIKI DESHMUKH Department of Botany, Yashavantrao Chavan Institute of Science, Satara, Maharashtra, India.
  • NAMDEO ANANDRAO GHANAWAT Department of Botany, Yashavantrao Chavan Institute of Science, Satara, Maharashtra, India.


Objective: The present study was aimed to investigate the mineral composition of Hardwickia binata Roxb.

Methods: Shade-dried powdered leaves, seed, and husk of H. binata were used for mineral analysis. Mineral analysis was carried out by acid digestion method. Potassium, calcium, and sodium were determined using a flame photometer (Thermofisher-FP114), whereas magnesium, zinc, iron, manganese, and copper were estimated using atomic absorption spectrophotometer (Thermofisher-AA203). Remaining elements namely phosphorus, sulfur, and boron were estimated by ultraviolet spectrophotometer and nitrogen by Kjeldahl’s apparatus.

Results: The total number of 13 mineral elements was found in H. binata. In case of values obtained from macroelements, magnesium content was found highest in leaves (51.05%), followed by husk (20.59%) and in seed (10.70%), whereas nitrate content was found lowest in seed (172.33 ppm), followed by leaves (158.17 ppm) and in husk (83.73 ppm). In case of microelements, iron was found maximum in husk (1246.48 ppm), followed by leaves (712.63 ppm) and in seed (157.39 ppm), whereas copper was found minimum in husk (5.73 ppm), followed by seed (4.92 ppm) and in leaves (4.68 ppm).

Conclusion: The present investigation of H. binata revealed promising source of magnesium, calcium, iron, and zinc. These elements may serve as nutritional supplement and could be beneficial to the human health as well as livestock to treat against deficiency disorders.

Keywords: Mineral elements, Hardwickia binata, Fabaceae/Caesalpiniaceae


1. Lalitha ST, Vijayalakshmi K. Proximate composition, nutritional evaluation and mineral analysis in the leaves of indigenous medicinal plant, Alternanthera sessilis. Int J Health Sci Res 2018;8:55-62.
2. Beegum GR, Sugunan VS, Beevy SS. Neutracuetical evaluation of Boerhavia diffusa L. Int J Curr Pharm Res 2017;9:101-4.
3. Talreja T, Sirohi P, Sharma T. Proximate composition analysis of two medicinally important plants Achyranthes aspera and Cissus quadrangularis. Int J Pharm Pharm Sci 2015;7:416-8.
4. Nazri NM, Hazali N, Ibrahim M, Masri M, Ayob MK. Preliminary studies on Acalypha indica: Proximate analysis and phytochemical screening. Int J Pharm Pharm Sci 2016;8:406-8.
5. Qotrunnada F, Ade A, Tejaputri NA, Qorina F. Antioxidative activity and phytochemistry profile of Hibiscus sabdariffa herb extracts. Int J Appl Pharm 2019;11:29-32.
6. Varmani A, Navneet, Prabhat, Chauhan A. Physico-chemical analysis of ash of some medicinal plants growing in Uttarakhand, India. Nature Sci 2010;8:88-91.
7. Seetharam YN, Kotresha K. Foliar venation of some species of Bauhinia L. and Hardwickia binata Roxb. (Caesalpinioideae). Phytomorphology 1998;48:51-9.
8. Vijaya SR, Ravikumar K, Goraya GS. Floristic wealth of Jawadhu hills, Eastern Ghats, with special emphasis on threatened plants. In: Special Habitats and Threatened Plants of India. Vol. 11. Dehradun: Wildlife Institute of India; 2008. p. 187-93.
9. Korwar GR. Hardwickia binate a promising MPTS, for agroforestry in Dryland areas. In: Multipurpose Tree Species for Agroforestry in India. Pune, India: BAIF Development Research Foundation Kamdhenu; 1994. p. 72-5.
10. Ranganathan R, Vijayalakshmi R, Parameswari P. Ethnomedicinal survey of Jawadhu hills in Tamil Nadu. Asian J Pharm Clin Res 2012;5:45-9.
11. Gunaselvi G, Kulasekaren V, Gopal V. Anti bacterial and antifungal activity of various leaves extracts of Hardwickia binata Roxb. (Caesalpinaceae). Int J PharmTech Res 2010;2:2183-7.
12. Khare CP. Indian Medicinal Plants. An Illustrated Dictionary. India: Springer; 2007. p. 302.
13. Prabakaran R, Kumar TS, Rao MV. GC-MS analysis and in vitro cytotoxicity studies of root bark exudates of Hardwickia binata Roxb. Am J Phytomed Clin Ther 2014;2:723-33.
14. Toth SJ, Prince AL, Wallace A, Mikkelsen DS. Rapid quantitative determination of eight mineral elements in plant tissue by a systematic procedure involving use of a flame photometer. Soil Sci 1948;66:459-66.
15. Ponmari M, Balasubiramanian KK. Evaluation of mineral contents in some medicinal plants used by traditional healers. Int J Res Pharm Pharm Sci 2017;2:30-4.
16. Preet R, Gupta RC, Pradhan SK. Elemental analysis and biological studies of Physalis angulata L. using wavelength-dispersive X-ray fluorescence technique, wavelength dispersion X-ray fluorescence, from Rajasthan. Asian J Pharm Clin Res 2017;10:220-4.
17. Malgorzata L, Agnieszka S. Recent advances in understanding plant response to sulfur-deficiency stress. Acta Bichim Pol 2008;55:457-71.
18. Prajna PS, Bhat PR. Phytochemical and mineral analysis of root of Loeseneriella arnottiana wight. Int J Curr Biosci Plant Biol 2015;2:67-72.
19. Indrayan AK, Sharma S, Durgapal D, Kumar N, Kumar M. Determination of nutritive value and analysis of mineral elements for some medicinally valued plants from Uttaranchal. Curr Sci 2005;89:1252-5.
20. Pattar M, Kerur BR, Nirmala C. Determination of some minerals and trace elements in medicinal plants Acalypha indica (L.), Datura metel (L.) and Tylophora indica used in the treatment of asthama. Eur J Med Plants 2018;22:1-11.
21. Sharanabasappa GK, Santosh MK, Shaila D, Seetharam YN, Sanjeevarao I. Phytochemical studies on Bauhinia racemosa Lam. Bauhinia purpurea Linn. and Hardwickia binata Roxb. E J Chem 2007;4:21-31.
22. Hefnawy AE, El-khaiat HM. The importance of copper and the effects of its deficiency and toxicity in animal health. Int J Livestock Res 2015;5:1-20.
23. Chaitanya AK, Pal B, Pati S, Badole S. Role of boron in crop production and its management. Pop Kheti 2014;2:38-41.
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How to Cite
DESHMUKH, S. V., and N. A. GHANAWAT. “QUANTIFICATION OF MINERAL ELEMENTS IN HARDWICKIA BINATA ROXB.-AN ENDEMIC PLANT”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 2, Feb. 2020, pp. 44-46, doi:10.22159/ajpcr.2020.v13i2.36180.
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