ANTIULCER ACTIVITY OF ELECTROHOMEOPATHIC DRUG (SPAGYRIC ESSENCE) CANCEROUS 15 - 3rd DILUTION AGAINST INDOMETHACIN-INDUCED GASTRIC ULCER IN RATS

  • PRASANT KUMAR SABAT Department of Pharmaceutics, School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan (Deemed to be) University, Bhubaneswar, Odisha, India.

Abstract

Objective: To evaluate the curative protects activity of electrohomeopathic drug Cancerous15 (C15) against Indomethacin (IND)-induced gastric ulcer in albino rats. None of the data have been reported on antiulcer activity of C15 drug. Hence, the present study focuses on the scientific investigation of antiulcer activity of C15 against Indomethacin-induced peptic ulceration of rat models.


Methods: A total number of 35 albino rats were divided into five groups equally. Pure water was given to Group 1 (normal). IND was given orally to Group 2 (ulcerated control). Cancerous15 third dilution was given to Group 3. IND was given to Group 4 and Group 5 animals, thereafter pretreatment with Esomeprazole (ESM) and C15-third dilution, respectively. The pretreatments were treated in each day’s interval for 21 days before IND administration. There was provision of food and water throughout the experimental period. On the 23rd day, the stomachs of the sacrificed rats were removed, and (1) ulcer index, (2) percentage of ulcer inhibition, (3) gastric volume, (4) pH, (5) pepsin activity, (6) mucin content, (7) malondialdehyde (MDA) concentration, and (8) stearidonic acid activity were studied.


Results: The pre-treated animals with ESM and C15 shown as significantly decreases in ulcer index, gastric volume, pepsin activity and MDA concentration, and significant increase in the percentage ulcer inhibition, pH value, mucin content, and superoxide dismutase activity concentration in comparison to ulcer-induced rats.


Conclusion: The study shows the significant ulcer protective activity of the C15 drug against IND-induced peptic ulceration in rats.

Keywords: Electrohomeopathy, Cancerous 15, Ulceration, Spagyric-medicine

References

1. Bhattacharya S, Chaudhuri SR, Chattopadhyay S, Bandyopadhyay SK. Healing properties of some Indian medicinal plants against indomethacin-induced gastric ulceration of rats. J Clin Biochem Nutr 2007;41:106-14.
2. Mishra S, Balaji O, Rao V, Adiga S. Gastroprotective activity of sesame (Sesamum indicum) seeds extract in alendronate-induced gastric ulcer in rats. Asian J Pharm Clin Res 2019;2:214-7.
3. Pandey D, Joshi A, Hemalata S. Anti ulcer study of standardized ethanolic root extract of Aganosma dichotoma and isolated urosuric acid. Int J Pharm Pharm Sci 2017;9:172-80.
4. Sanyal AR, Denath OK, Bhattacharya SK, Gode KD. The effect of cyproheptadine on gastric acidity. In: Pfeiffer CJ, editor. Peptic Ulcer. Munksgoard: Scandinavian University Books; 1971. p. 312-8.
5. Corne SJ, Morrissey SM, Woods RJ. Proceedings: A method for the quantitative estimation of gastric barrier mucus. J Physiol 1974;242:116-7.
6. Szabo S, Hollander D. Pathways of gastrointestinal protection and repair: Mechanisms of action of sucralfate. Am J Med 1989;86: 23-31.
7. Sayed DA, Fahmi SR, Soliman AM, Hussin NS. Antiulcerogenic efficacy of ethanolic extract of Vitis vinifera leaves in rats. Int J Pharm Pharm Sci 2016;8:63-172.
8. Lichtenberger LM. The hydrophobic barrier properties of gastrointestinal mucus. Annu Rev Physiol 2005;17:178-88.
9. Inas ZA, Abdallah-Hala AH, Khattab H, Gehan HH. Gastroprotective effect of Cordia myxa L. fruit extract against indomethacin-induced gastric ulceration in rats. Life Sci J 2011;8:433-45.
10. Ajani EO, Sabiu S, Bamisaye FA, Adenigba BV, Awomoyi DD, Adeyanju MN. Hepatoprotective and antioxidative effect of ethanolic leaf extract of Langenaria breviflora (bitter gourd) on indomethacin-ulcerated rats. J Pharm Biol Sci 2014;9:61-8.
11. Raji Y, Oyeyemi WA, Shittu ST, Bolarinwa AF. Gastro-protective effect of methanol extract of Ficus asperifolia bark on indomethacin-induced gastric ulcer in rats. Niger J Physiol Sci 2011;26:43-8.
12. Adhikary B, Yadav SK, Roy K, Bandyopadhyay SK, Chattopadhyay S. Black tea and theaflavins assist healing of indomethacin-induced gastric ulceration in mice by antioxidative action. Evid Based Complement Alternat Med 2011;2011:546560.
13. Lüllmann H, Mohr K, Ziegler A, Bieger D. Color Atlas of Pharmacology. 2nd ed. New York: Thieme Stuttgart; 2000. p. 166.
14. Beck PL, Xavier R, Lu N, Nanda NN, Dinauer M, Podolsky DK, et al. Mechanisms of NSAID-induced gastrointestinal injury defined using mutant mice. Gastroenterology 2000;119:699-705.
15. Muhammed AV, Thamotharan G, Sengottuvelu S, Haja-Sherief S, Sivakumar T. Evaluation of antiulcer activity of Ficus pumila L. leaf extract in albino rats. Glob J Res Med Plants Indig Med 2012;1:340-51.
16. Naito Y, Yoshikawa T, Matsuyama K, Yagi N, Arai M, Nakamura Y, et al. Effects of oxygen radical scavengers on the quality of gastric ulcer healing in rats. J Clin Gastroenterol 1995;21 Suppl 1:S82-6.
Statistics
180 Views | 115 Downloads
Citatons
How to Cite
SABAT, P. K. “ANTIULCER ACTIVITY OF ELECTROHOMEOPATHIC DRUG (SPAGYRIC ESSENCE) CANCEROUS 15 - 3rd DILUTION AGAINST INDOMETHACIN-INDUCED GASTRIC ULCER IN RATS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 2, Dec. 2019, pp. 174-7, doi:10.22159/ajpcr.2020.v13i2.36181.
Section
Original Article(s)