EVALUATION OF ANTI-INFLAMMATORY, ANALGESIC, ANTIPYRETIC EFFECT OF EICOSANE, PENTADECANE, OCTACOSANE, AND HENEICOSANE

  • PATRICK NWABUEZE OKECHUKWU Department of Biotechnology, Faculty of Applied Science, UCSI University No. 1, Jalan Menara Gading, UCSI Heights, (Taman Connaught) Cheras 56000 Kuala Lumpur, Malaysia.

Abstract

Objective: Marantodes pumilum (MP) is a plant widely used in traditional medicine in the Southeast Asian region and been reported to possess anti-inflammatory, analgesic, antipyretic, and antiulcer properties. The aim of the study is to identify the bioactive phytochemicals present in the purified leaf extract of MP using gas chromatography mass spectrometry (GC-MS), and to determine their anti-inflammatory, analgesic, and antipyretic effect in a rat model.


Methods: A dichloromethane crude extract of MP was partially purified using column chromatography, and the bioactive compounds were identified using GC-MS. The anti-inflammatory, analgesic, and antipyretic activities of the identified bioactive compounds were evaluated using formalin, carrageenan, acetic acid, and brewer’s yeast to induce the rats.


Results: Eicosane, pentadecane, octacosane, and heneicosane were identified as bioactive compounds in purified leaf extract of MP. These bioactive compounds did not show any toxicity on the rats at 1000 mg/kg. There was no mortality, and no apparent behavioral, physiological, and morphological changes of the internal organs occurred. They exhibited a very significant (p<0.05) dose-dependent inhibition of acetic acid-induced writhing, formalin-induced paw licking, carrageenan-induced paw edema, and brewer’s yeast-induced pyrexia.


Conclusions: Eicosane and pentadecane were able to show very strong anti-inflammatory, analgesic, and antipyretic effects. The observed effects of the bioactive compounds are similar to previous reports on crude and partially purified leaf extract of MP. The finding suggests that eicosane and pentadecane maybe the major bioactive compounds present in the purified leaf extract of MP.

Keywords: Marantodes pumilum, Anti-inflammatory, Analgesic, Antipyretic, Toxicity

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OKECHUKWU, P. N. “EVALUATION OF ANTI-INFLAMMATORY, ANALGESIC, ANTIPYRETIC EFFECT OF EICOSANE, PENTADECANE, OCTACOSANE, AND HENEICOSANE”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 4, Feb. 2020, pp. 29-35, doi:10.22159/ajpcr.2020.v13i4.36196.
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