EVALUATION OF ANTI-INFLAMMATORY, ANALGESIC, ANTIPYRETIC EFFECT OF EICOSANE, PENTADECANE, OCTACOSANE, AND HENEICOSANE
Keywords:Marantodes pumilum, Anti-inflammatory, Analgesic, Antipyretic, Toxicity
Objective: Marantodes pumilum (MP) is a plant widely used in traditional medicine in the Southeast Asian region and been reported to possess anti-inflammatory, analgesic, antipyretic, and antiulcer properties. The aim of the study is to identify the bioactive phytochemicals present in the purified leaf extract of MP using gas chromatography mass spectrometry (GC-MS), and to determine their anti-inflammatory, analgesic, and antipyretic effect in a rat model.
Methods: A dichloromethane crude extract of MP was partially purified using column chromatography, and the bioactive compounds were identified using GC-MS. The anti-inflammatory, analgesic, and antipyretic activities of the identified bioactive compounds were evaluated using formalin, carrageenan, acetic acid, and brewer’s yeast to induce the rats.
Results: Eicosane, pentadecane, octacosane, and heneicosane were identified as bioactive compounds in purified leaf extract of MP. These bioactive compounds did not show any toxicity on the rats at 1000 mg/kg. There was no mortality, and no apparent behavioral, physiological, and morphological changes of the internal organs occurred. They exhibited a very significant (p<0.05) dose-dependent inhibition of acetic acid-induced writhing, formalin-induced paw licking, carrageenan-induced paw edema, and brewer’s yeast-induced pyrexia.
Conclusions: Eicosane and pentadecane were able to show very strong anti-inflammatory, analgesic, and antipyretic effects. The observed effects of the bioactive compounds are similar to previous reports on crude and partially purified leaf extract of MP. The finding suggests that eicosane and pentadecane maybe the major bioactive compounds present in the purified leaf extract of MP.
Harizal SN, Mansor SM, Hasnan J, Tharakan JK, Abdullah J. Acute toxicity study of the standardized methsanolic extract of Mitragyna speciosa Korth in rodent. J Ethnopharmacol 2010;131:404-9.
Atsamo AD, Nguelefack TB, Datté JY, Kamanyi A. Acute and subchronic oral toxicity assessment of the aqueous extract from the stem bark of Erythrina senegalensis DC (Fabaceae) in rodents. J Ethnopharmacol 2011;134:697-702.
Adedapo AA, Sofidiya MO, Maphosa V, Moyo B, Masika PJ, Afolayan AJ. Anti-inflammatory and analgesic activities of aqueous
extract of Cussonia paniculata stem bark. Rec Nat Prod 2008;2:46-53.
Kim HP, Son KH, Chang HW, Kang SS. Anti-inflammatory plant flavonoids and cellular action mechanisms. J Pharmacol Sci 2004;96:229-45.
Jamal W, Mohammed MS, Ahmed WJ, Khalid HS, Mahmoud AM, Garelnabi EA. Di-(2’-ethylhexyl) phthalate and stigmasterol with anti-inflammatory effect from Cyperus rotundus L. Int J Pharm Chem Biol Sci 2014;4:453-9.
Hisham DM, Lip JM, Noh JM, Normah A. Identification and isolation of methyl gallate as a polar chemical marker for Labisia pumila Benth. J Trop Agric Food Sci 2011;39:279-84.
Okechukwu PN, Ekeuku SO, Loshnie S, Akowuah GA. Anti-inflammatory, analgesic, antinociceptive and antipyretic investigation of bioactive constituents from partial purified dichloromethane crude extracts from leaves of Labisia pumila. Int J Pharm Res Scholars 2014;3:743-51.
Stein SE. An integrated method for spectrum extraction. J Am Soc Mass Spectrom 1999;10:770-81.
Sharifi-Rad J, Seyedeh MH, Majid SR, Setzer WN. Chemical composition, antifungal and antibacterial activities of essential oil from Lallemantia royleana (Benth. in Wall.) Benth J Food Saf 2014;35:19-25.
Rana IS, Rana AS, Rajak RC. Evaluation of antifungal activity in essential oil of the Syzygium aromaticum (L.) by extraction, purification and analysis of its main component eugenol. Braz J Microbiol 2011;42:1269-77.
Sacchetti G, Maietti S, Muzzoli M, Scaglianti M, Manfredini S, Radice M, et al. Comparative evaluation of 11 essential oils of different origin as functional antioxidants, antiradicals and antimicrobials in foods. Food Chem 2005;91:621-32.
Samuagam L, Akowuah GA, Okechukwu PN. Partial purification and antinociceptive investigation of extracts of leaves of Labisia pumila. Asian J Pharm Clin Res 2011;4:44-6.
Sulaiman MR, Perimal EK, Akhtar MN, Mohamad AS, Khalid MH, Tasrip NA, et al. Anti-inflammatory effect of zerumbone on acute and chronic inflammation models in mice. Fitoterapia 2010;81:855-8.
Okechukwu PN, Ikujuni CM. The study of anti-inflammatory anti-pyretic and anti-nociceptive activities of extract from leaves of Labisia pumila. Int J Pharmacol Toxicol Sci 2012;2:12-27.
Ekeuku SO, Okechukwu PN. Paw edemaand bronchoconstriction induced by mediators: How they were inhibited in vivo by partially purified extract from dichloromethane leaf extract of Labisia pumila. Middle East J Sci Res 2014;22:1114-21.
Creton S, Dewhurst IC, Earl LK, Gehen SC, Guest RL, Hotchkiss JA, et al. Acute toxicity testing of chemicals-opportunities to avoid redundant testing and use alternative approaches. Crit Rev Toxicol 2010;40:50-83.
Akhila JS, Shyamjith D, Alwar MC. Acute toxicity studies and determination of median lethal dose. Curr Sci 2007;93:917-20.
Gad SC. Reference Module in Biomedical Sciences Encyclopedia of Toxicology. 3rd ed. North Carolina: Elsevier; 2014. p. 58-60.
Bentley GA, Newton SH, Starr J. Evidence for an action of morphine and the enkephalins on sensory nerve endings in the mouse peritoneum. Br J Pharmacol 1981;73:325-32.
Siegmund E, Cadmus R, Lu G. A method for evaluating both non-narcotic and narcotic analgesics. Proc Soc Exp Biol Med 1957;95:729-31.
Satyanarayana PS, Jain NK, Singh A, Kulkarni SK. Isobolographic analysis of interaction between cyclooxygenase inhibitors and tramadol in acetic acid-induced writhing in mice. Prog Neuropsychopharmacol Biol Psychiatry 2004;28:641-9.
Wang MQ, Ge BS, Yang QX, Jiang XY, Huang F. High-level production of biologically active chemokines in Escherichia coli. Process Biochem 2014;49:706-714.
Demir Özkay U, Can OD. Anti-nociceptive effect of vitexin mediated by the opioid system in mice. Pharmacol Biochem Behav 2013;109:23-30.
Pinheiro MM, Bessa SO, Fingolo CE, Kuster RM, Matheus ME, Menezes FS, et al. Antinociceptive activity of fractions from Couroupita guianensis Aubl. Leaves. J Ethnopharmacol 2010;127:407-13.
Pinheiro MM, Boylan F, Fernandes PD. Antinociceptive effect of the Orbignya speciosa mart. (babassu) leaves: Evidence for the involvement of apigenin. Life Sci 2012;91:293-300.
Reuben S, Lopez M, Maciolek H, Fortunato A. NSAIDs and acetaminophen: Postoperative analgesic effects of celecoxib for spinal fusion surgery. J Pain 2004;5:S64.
Robinson C, Alfonso H, Woo S, Olsen N, Bill Musk AW, Robinson BW, et al. Effect of NSAIDS and COX-2 inhibitors on the incidence and severity of asbestos-induced malignant mesothelioma: Evidence from an animal model and a human cohort. Lung Cancer 2014;86:29-34.
Castro P, Nasser H, Abrahão A, Dos Reis LC, Riça I, Valença SS, et al. Aspirin and indomethacin reduce lung inflammation of mice exposed to cigarette smoke. Biochem Pharmacol 2009;77:1029-39.
Sadowsky DW, Haluska GJ, Gravett MG, Witkin SS, Novy MJ. Indomethacin blocks interleukin 1beta-induced myometrial contractions in pregnant rhesus monkeys. Am J Obstet Gynecol 2000;183:173-80.
Wheeler-Aceto H, Cowan A. Neurogenic and tissue-mediated components of formalin-induced edema: Evidence for supraspinal regulation. Agents Actions 1991;34:264-9.
Hunskaar S, Hole K. The formalin test in mice: Dissociation between inflammatory and non-inflammatory pain. Pain 1987;30:103-14.
Shibata M, Ohkubo T, Takahashi H, Inoki R. Modified formalin test: Characteristic biphasic pain response. Pain 1989;38:347-52.
Abbott FV, Franklin KB, Westbrook RF. The formalin test: Scoring properties of the first and second phases of the pain response in rats. Pain 1995;60:91-102.
Santos AR, Calixto JB. Further evidence for the involvement of tachykinin receptor subtypes in formalin and capsaicin models of pain in mice. Neuropeptides 1997;31:381-9.
Rachchh M, Yadav P, Gokani R, Jain S. Anti-inflammatory activity of Benincasa hispida fruit. Int J Pharm Bio Sci 2011;2:98-106.
Sadeghi H, Haijhashemi V, Minaiyan M, Movahedian A, Talebi A. Further studies on anti-inflammatory activity of maprotiline in carrageenan-induced paw edema in rat. Int Immunopharmacol 2013;15:505-10.
Reis EF, Castro SB, Alvesm CC, Oliveira EE, Correa TA, Almeida MV, et al. Lipophilic amino alcohols reduces carrageenan-induced paw edema and anti-OVA DTH in BALB/c mice. Int Immunopharmacol 2013;17:727-32.
Solanki HK, Shah DA, Maheriya PM, Patel CA. Evaluation of anti-inflammatory activity of probiotic on carrageenan-induced paw edema in Wistar rats. Int J Biol Macromol 2015;72:1277-82.
Yun KJ, Koh DJ, Kim SH, Park SJ, Ryu JH, Kim DG, et al. Anti-inflammatory effects of sinapic acid through the suppression of inducible nitric oxide synthase, cyclooxygase-2, and proinflammatory cytokines expressions via nuclear factor-κB inactivation. J Agric Food Chem 2008;56:10265-72.
Tohda C, Nakayama N, Hatanaka F, Komatsu K. Comparison of anti-inflammatory activities of six curcuma rhizomes: A possible curcuminoid-independent pathway mediated by Curcuma phaeocaulis extract. Evid Based Complement Alternat Med 2006;3:255-60.
Saper CB, Breder CD. The neurologic basis of fever. N Engl J Med 1994;330:1880-6.
Luheshi, GN. Cytokines and fever. Mechanisms and sites of action. Ann N Y Acad Sci 1998;856:83-9.
Bhat AS, Tandan SK, Kumar D, Krishna V, Prakash VR. Interaction between inhibitors of inducible nitric oxide synthase and cyclooxygenase in Brewer’s yeast induced pyrexia in mice: An isobolographic study. Eur J Pharmacol 2005;511:137-42.
Zhang YH, Elmquist JK, Saper CB. Specific roles of cyclooxygenase-1 and cyclooxygenase-2 in lipopolysaccharide-induced fever and fos expression in rat brain. J Comp Neurol 2003;463:3-12.
Simon LS. Role and regulation of cyclooxygenase-2 during inflammation. Am J Med 1999;106:37S-42.
Vane JR. Inhibition of prostaglandin synthesis as a mechanism of action for aspirin-like drugs. Nat N Biol 1971;231:232-5.
Popovich PG, Tovar CA, Wei P, Fisherm L, Jakeman LB, Basso DM. A reassessment of a classic neuroprotective combination therapy for spinal cord injured rats: LPS/pregnenolone/indomethacin. Exp Neurol 2012;233:677-85.
Aronoff DM, Neilson EG. Antipyretics: Mechanisms of action and clinical use in fever suppression. Am J Med 2001;111:304-15.
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