IN-VITRO THROMBOLYTIC AND CYTOTOXIC ACTIVITY OF METHANOLIC EXTRACT OF FLEMINGIA MACROPHYLLA LEAVES
An investigation was made to find out the cytotoxic and thrombolytic activity of methanolic extract of leaves of Flamingia macrophylla. In-vitro test to observe thrombolytic and cytotoxic potential was done with the methanolic extract of F. macrophylla in this purpose. Streptokinase was used as positive control and water as negative control in the methodology of thrombolytic testing. In case of cytotoxic activity observation, Brine shrimp lethality bioassay testing method was used, 5% DMSO and brine shrimps were used as test materials, where DMSO as solvent for proper solution. The percent of thrombolysis by the extract showed 43.067 Â± 3.0601%, whereas percent of thrombolysis by streptokinase was found 68.582 Â± 1.7764%. From the cytotxicity study of the crude extract LC50 was found 1.981Âµg/ml with 95% confidence limit was 1.7299-2.2706 Âµg/ml. The current study refers the plant leaves as impressive thrombolytic and cytotoxic agent for further laboratory study.
Keywords: Flemingia macrophylla, thrombolytic, Cytotoxicity, DMSO, 95% confidence limit.
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