IN-VITRO THROMBOLYTIC AND CYTOTOXIC ACTIVITY OF METHANOLIC EXTRACT OF FLEMINGIA MACROPHYLLA LEAVES

Authors

  • Mohammed Abdullah Jainul
  • Shofiul Azam
  • Amin Chowdhury
  • Rukhon Uddin Mubarak
  • Kazi Omar Faruq

Abstract

An investigation was made to find out the cytotoxic and thrombolytic activity of methanolic extract of leaves of Flamingia macrophylla. In-vitro test to observe thrombolytic and cytotoxic potential was done with the methanolic extract of F. macrophylla in this purpose. Streptokinase was used as positive control and water as negative control in the methodology of thrombolytic testing. In case of cytotoxic activity observation, Brine shrimp lethality bioassay testing method was used, 5% DMSO and brine shrimps were used as test materials, where DMSO as solvent for proper solution. The percent of thrombolysis by the extract showed 43.067 ± 3.0601%, whereas percent of thrombolysis by streptokinase was found 68.582 ± 1.7764%. From the cytotxicity study of the crude extract LC50 was found 1.981µg/ml with 95% confidence limit[1] was 1.7299-2.2706 µg/ml. The current study refers the plant leaves as impressive thrombolytic and cytotoxic agent for further laboratory study.

Keywords: Flemingia macrophylla, thrombolytic, Cytotoxicity, DMSO, 95% confidence limit.

References

Matsumura, F; (1985); Toxicology of Insecticides; 2nd ed; Plenum,. pp. 14-16.

Flora of China Editorial Committee, Flora of China (Fabaceae) (10th ed.); (2010); Science Press & Missouri Botanical Garden Press, Beijing & St. Louis; pp. 1–642.

Gutteridge, RC, Shelton, HM; (1994); Forage Tree Legumes in Tropical Agriculture; CAB International, Wallingford, UK.

Orwa, C, Mutua, A, Kindt, R, Jamnadass, R, Anthony, S; (2009); Agroforestree Database: a Tree Reference and Selection Guide; Version 4.0 (Flemingia macrophylla); World Agroforestry Center; pp. 1-5.

http:www.mpbd.info/plants/flemingia-macrophylla/

http://www.tropicalforages.info/key/Forages/Media/Html/Flemingia_macrophylla/

Sikri, N, Bardia, A; (2007) "A history of streptokinase use in acute myocardial infarction". Tex Heart Inst J; 34 (3): 318–27.

Micharl Dockey & Stephen Tonkins. "Brine shrimp ecology". British Ecological Society.

Prasad, S, Kashyap, RP, Deopujari, JY, Purohit, HJ, Taori, GM and Daginawala, HF; (2007); Effect of Fagonia Arabica (Dhamasa) on in vitro thrombolysis; BMC, Complementary and Alternative Medicine; 7:36 doi:10.1186/1472-6882-7-36

Mayer, BN, Ferrigni, NR, Putnam, JE, Jacobsen, LB, Nichols, DE and Mclaughlin, JL (1982); Brine shrimp: a convenient bioassay for active plant constituents; Plant Medica; 45:31-34.

Jainul, MA; Azam, S; Chowdhury, A; (2013) In-vitro cytotoxic activity of methanolic extract of M. pachycarpa Benth. leaves; The Pharma Innovation; vol-2, issue-1, pp:10-13.

Published

2013-09-01

How to Cite

Jainul, M. A., S. Azam, A. Chowdhury, R. U. Mubarak, and K. O. Faruq. “IN-VITRO THROMBOLYTIC AND CYTOTOXIC ACTIVITY OF METHANOLIC EXTRACT OF FLEMINGIA MACROPHYLLA LEAVES”. Asian Journal of Pharmaceutical and Clinical Research, vol. 6, no. 8, Sept. 2013, pp. 86-88, https://innovareacademics.in/journals/index.php/ajpcr/article/view/362.

Issue

Section

Articles