SCREENING OF PHYTOCHEMICAL CONTENT AND IN VITRO BIOLOGICAL INVESTIGATION OF CANTHIUM DICOCCUM (GAERTN.) AND AMISCHOPHACELUS AXILLARIS (L.)
DOI:
https://doi.org/10.22159/ajpcr.2020.v13i1.36200Keywords:
Antihypertensive, Anthelmintic, Canthium dicoccum (Gaertn), Amischophacelus axillaris (L), Angiotensin-Converting Enzyme inhibitorsAbstract
Objective: The objective of the study was to study the pet ether, ethyl acetate, and ethanol leaf extracts of Canthium dicoccum and Amischophacelus axillaris for anthelmintic activity and antihypertensive activity.
Methods: The antihypertensive activity was carried out by employing a colorimetric assay based on the hydrolysis of Histidyl-Hippuryl-Leucine and anthelmintic activity carried out against Indian earthworm Pheritimaposthuma.
Results: The pet ether leaf extract both the plants exhibited the maximum antihypertensive activity with a percent inhibition of 64.82 for C. dicoccum (Gaertn.) and 84.12 for A. axillaris (L.) as compared with Captopril showing percent inhibition 85.37 and for anthelmintic activity, it is found that ethanol extract of C. dicoccum and ethyl acetate extract of A. axillaris exhibited significant activity against the standard drug albendazole.
Conclusion: This study investigated the potential of C. dicoccum and A. axillaris as a new source against the antihypertensive activity. The outcome of anthelmintic activity revealed that the ethyl acetate and ethanol extracts exhibited a considerable amount of anthelmintic activity, which is mainly due to the active phytoconstituents present in the extracts.
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Amery A, Birkenhäger W, Brixko P, Bulpitt C, Clement D, Deruyttere M, et al. Mortality and morbidity results from the European working party on high blood pressure in the elderly trial. Lancet 1985;1:1349-54.
Dahlöf B, Lindholm LH, Hansson L, Scherstén B, Ekbom T, Wester PO. Morbidity and mortality in the Swedish Trial in Old Patients with Hypertension (STOP-Hypertension) Lancet 1991;338:1281-5.
Lewis WH, Lewis MP. Medicinal Botany Plants Affecting Man’s Health. New York: John Wiley and Sons; 1977. p. 515.
Staessen JA, Fagard R, Thijs L, Celis H, Arabidze GG, Birkenhäger WH, et al. Randomised double-blind comparison of placebo and active treatment for older patients with isolated systolic hypertension. The Systolic Hypertension in Europe (Syst-Eur) Trial Investigators. Lancet 1997;350:757-64.
Dhar DN, Sharma RL, Bansal GC. Gastro-intestinal nematodes in sheep in Kashmir. Vet Parasitol 1982;11:271-7.
Bundy DA. Immunoepidemiology of intestinal helminthic infections. 1. The global burden of intestinal nematode disease. Trans R Soc Trop Med Hyg 1994;88:259-61.
Satyavati GV, Raina MK, Sharma M. Medicinal Plants of India. New Delhi, India: Indian Council of Medical Research; 1976. p. 201-6.
Rajeswari NR, Ramalakshmi S, Muthuchelian K. GC-MS Analysis of bioactive components from the ethanolic leaf extract of Canthium dicoccum (Gaertn.). J Chem and Pharm Res 2011;3:792-8.
Neelima M, Prasad GP, Sudarsanam G, Penchala PG, Jothi B. Ethnobotanical studies in rapur forest division of Nellore district in Andhra Pradesh. Life Sci Lealf 2011;11:333-45.
Santhan S, Janarthan M, Ali MZ. Evaluation of antidiabetic and nephroprotective activity of 95% ethanol extract of Canthium dicoccum whole plant by using albino rats. J Chem Pharm Sci 2013;6:218-22.
Gupta AK. Cyanotis axillaris. The IUCN Red List of Threatened Species; 2011.
Vidyarthi RD, Pandey PN. Textbook of Zoology. Vol. 14. New Delhi: Chand and Company; 2005. p. 329.
Thorn GW, Adams RD, Brauwald EK, Isselbacher KJ, Petersdoft RG. Harrison’s Principles of Internal Medicine. Vol. 10. New York: McGraw Hill and Company; 1977. p. 1088.
Harborne JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. Vol. 3. Boca Raton: Chapman and Hall Publication; 1998. p. 11.
Jimsheena VK, Gowda LR. Colorimetric, high-throughput assay for screening Angiotensin I-converting enzyme inhibitors. Anal Chem 2009;81:9388-94.
Hooper NM, Turner AJ. Isolation of two differentially glycosylated forms of peptidyl-dipeptidase A (angiotensin converting enzyme) from pig brain: A re-evaluation of their role in neuropeptide metabolism. Biochem J 1987;241:625-33.
Thorn GW, Adams RD, Brauwald EK. Harrison’s Principles of Internal Medicine. New York: McGraw Hill and Company; 1977. p. 1088.
Vigar Z. Atlas of Medical Parasitology. Vol. 2. Singapore: PG Publishing House; 1984. p. 216.
Toker G, Kupeli E, Memizolu M, Yesilada EJ. Ethnopharmacol 2004;95:393.
Satyanarayana V, Kumari SJ. Preliminary phytochemical screening and antioxidant activity of selected four plants. Int J Green Pharm 2017;11:S116.
Loizzo MR, Tundis R, Menichini F, Statti GA, Menichini F. Hypotensive natural products: Current status. Mini Rev Med Chem 2008;8:828-55.
Elbl G, Wagner H. A new method for the in vitro screening of inhibitors of angiotensin-converting enzyme (ACE), using the chromophore and fluorophore-labelled substrate, dansyltriglycine. Planta Med 1991;57:137-41.
Girirajasekhar D, Prasanna DG, Chandrakanth P. Prescribing pattern of antihypertensive drugs based on compelling indications with hypertension. Int J Pharm Pharm Sci 2016;8:72-5.
Shil D, Laloo D, Das SR, Dash S. Pharmacognostical and phytochemical standardization of Physalis minima L. Leaf. Int J Pharm Pharm Sci 2019;11:20-5.
Thompson DP, Geary TG. The structure and function of helminths surfaces. In: Marr JJ, editor. Biochemistry and Molecular Biology of Parasites. Cambridge: Academic Press; 1995. p. 203-32.
Iqbal Z, Sarwar M, Jabbar A, Ahmed S, Nisa M, Sajid MS, et al. Direct and indirect anthelmintic effects of condensed tannins in sheep. Vet Parasitol 2007;144:125-31.
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