• ANANTHULA MOUNIKA Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Hyderabad, Telangana, India.
  • BHARGAVI POKALA Equine Veterinary Medical Centre, Qatar Foundation, Education City, Doha, Qatar.
  • ZAHOORULLAH S MD. Atal Incubation Centre, Aleap Wehub, Aleap Industrial Estate, Hyderabad, Telangana, India.
  • ANJANEYULU MUSINI Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Hyderabad, Telangana, India.


Objective: Salacia oblonga Wall. is an important medicinal plant belonging to the family Celastraceae. The study reports the effect of S. oblonga root extracts against clinical isolate Staphylococcus aureus

Methods: Antibacterial activity was evaluated by agar diffusion method and assay for minimum inhibitory concentration (MIC) of extract. Further, the effect of S. oblonga extract determined by DNA fragmentation and respiratory dehydrogenase enzyme activity assays.

Results: S. oblonga ethyl acetate root extract was evaluated for antibacterial activity towards clinical isolate S. aureus. Bacterial growth was determined in treated and control cells. Extract displayed good growth inhibition and MIC of the extract was 80 μg/ml. DNA fragmentation assay was carried out, this result has shown that treated bacterial cell has DNA damage compared to the control cell. Further, respiratory dehydrogenase enzyme activity was determined. In the treated cells, enzyme activity was low compared to the control cells.

Conclusion: Salacia oblonga root extract inhibiting the growth of S. aureus by different modes of action.

Keywords: Clinical isolate, Staphylococcus aureus, DNA fragmentation assay, Respiratory dehydrogenase, Salacia oblonga


1. Bhatia R, Narain JP. The growing challenge of antimicrobial resistance in the South-East Asia Region-are we losing the battle? Indian J Med Res 2010;132:482-6.
2. Boucher HW, Talbot GH, Bradley JS, Edwards JE, Gilbert D, Rice LB, et al. Bad bugs, no drugs: No ESKAPE! An update from the infectious diseases society of America. Clin Infect Dis 2009;48:1-2.
3. Giamarellou H. Multidrug-resistant gram-negative Bacteria: How to treat and for how long. Int J Antimicrob Agents 2010;36:50-4.
4. Oguntibeju OO. Antidiabetic, anti-inflammatory, antibacterial, anti-helminthic, antioxidant and nutritional potential of musa paradisiacal. Asian J Pharm Clin Res 2019;12:19-3.
5. Amulkars S, Tale V, Khilari S, Pawar J. Antibacterial and antibiofilm activity of Quercus Infectoria gallls on Rothia Dentocariosa isolated from dental caries. Asian J Pharm Clin Res 2019;12:159-62.
6. Chawla A, Singh S, Sharma AK. Salacia oblonga wall: A review on its pharmacognostic, phytochemical and pharmacological aspects. Int J Res Pharm Biomed Sci 2013;4:1215-28.
7. Li Y, Huang TH, Yamahara J. Salacia root, a unique ayurvedic medicine, meets multiple targets in diabetes and obesity. Life Sci 2008;82:1045-9.
8. Ismail TS, Gopalakrishnan S, Begum VH, Elango V. Anti-inflammatory activity of Salacia oblonga wall. And Azima tetracantha Lam. J Ethnopharmacol 1997;56:145-52.
9. Musini A, Rao JP, Giri A. Isolation of potential antimicrobial compounds from Salacia oblonga Wall and their synergistic effect on human pathogens. J Microbiol Biotech Food Sci 2015;5:7-11.
10. Perez CP, Bazerque PM. An antibiotic assay by the well agar method. Acta Biol Med Exp 1990;15:113-5.
11. Li WR, Xie XB, Shi QS, Zeng HY, Ou-Yang YS, Chen YB. Antibacterial activity and mechanism of silver nanoparticles on Escherichia coli Appl Microbiol Biotechnol 2010;85:1115-22.
12. Radulovi? NS, Deki? MS, Stojanovi?-Radi? ZZ, Zorani? SK. Geranium macrorrhizum L. (Geraniaceae) essential oil: A potent agent against Bacillus subtilis. Chem Biodivers 2010;7:2783-800.
13. van Vuuren S, Viljoen A. Plant-based antimicrobial studies--methods and approaches to study the interaction between natural products. Planta Med 2011;77:1168-82.
14. Anandhi D, Srinivasan PT, Kumar P, Jagatheesh S. DNA fragmentation induced by the glycosides and flavonoids from C. Coriaria. Int J Curr Microbiol App Sci 2014;3:666-73.
15. Tapia L, Torres J, Mendoza L, Urzúa A, Ferreira J, Pavani M, et al. Effect of 13-epi-sclareol on the bacterial respiratory chain. Planta Med 2004;70:1058-63.
185 Views | 101 Downloads
How to Cite
ANANTHULA MOUNIKA, BHARGAVI POKALA, Z. S. MD., and ANJANEYULU MUSINI. “EFFECT OF SALACIA OBLONGA ROOT EXTRACT AGAINST CLINICAL ISOLATES STAPHYLOCOCCUS AUREUS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 1, Nov. 2019, pp. 171-3, doi:10.22159/ajpcr.2020.v13i1.36245.
Original Article(s)