A NOVEL REVERSE-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR SIMULTANEOUS ESTIMATION OF ALOE-EMODIN AND PIPERINE IN AYURVEDIC FORMULATION

  • LEENA TANDEL Department of Quality Assurance, Oriental College of Pharmacy, Navi Mumbai, Maharashtra, India.
  • REVATI SONONE Department of Quality Assurance, Oriental College of Pharmacy, Navi Mumbai, Maharashtra, India.
  • VANDANA JAIN Department of Quality Assurance, Oriental College of Pharmacy, Navi Mumbai, Maharashtra, India.

Abstract

Objective: The objective of this study was to develop and validate a novel, simple, rapid, accurate, and precise reverse-phase high-performance liquid chromatographic (RP-HPLC) method for simultaneous estimation of aloe-emodin and piperine in the ayurvedic formulation as per the International Conference on Harmonization guidelines.


Methods: Chromatographic separation was achieved using a Prontosil C18 (250 × 4.6 mm, 5 μ), with a mobile phase consisting of 0.05% orthophosphoric acid and acetonitrile in the ratio of 50:50, at a flow rate of 1 ml/min and column temperature maintained at 28°C and ultraviolet (UV) detection at 225 nm.


Results: The retention time of aloe-emodin and piperine was found to be 9.38±0.2 min and 13.45±0.2 min, respectively. The linearity of aloe-emodin and piperine was tested in the range of 1–20 μg/ml. The correlation coefficient for aloe-emodin and piperine was found to be 0.998 and 0.997, respectively. The recovery values (98–102%) indicate a satisfactory accuracy. The percentage relative standard deviation for precision was found to be <2% which indicates that the method is precise.


Conclusion: A precise method for the simultaneous quantification of aloe-emodin and piperine was developed using high-performance liquid chromatography. Hence, the developed method can be used for quantitative and quality control analysis of formulations containing these phytoconstituents.

Keywords: Aloe-emodin, Piperine, Ayurvedic formulation, Reverse-phase high-performance liquid chromatographic, Validation

Author Biography

LEENA TANDEL, Department of Quality Assurance, Oriental College of Pharmacy, Navi Mumbai, Maharashtra, India.

Associate Professor at oriental college of pharmacy, recipient of ugc-jrf in engg and tech.....successfully completed 2 minor research project granted by mumbai university.......several publications in national and international journal of good impact factor...

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TANDEL, L., R. SONONE, and V. JAIN. “A NOVEL REVERSE-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR SIMULTANEOUS ESTIMATION OF ALOE-EMODIN AND PIPERINE IN AYURVEDIC FORMULATION”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 2, Dec. 2019, pp. 51-54, doi:10.22159/ajpcr.2020.v13i2.36326.
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