PRELIMINARY PHYTOCHEMICAL SCREENING, CHARACTERIZATION, AND ANTIDIABETIC ACTIVITY OF LEAF EXTRACT OF CANNA INDICA L. IN STREPTOZOTOCIN-INDUCED DIABETIC MODEL

  • PRASHANT KUMAR YADAV Department of Pharmacy, Faculty of Pharmacy, Bhupal Nobles University, Udaipur, Rajasthan, India.
  • SISODIA SS Department of Pharmacy, Faculty of Pharmacy, Bhupal Nobles University, Udaipur, Rajasthan, India.

Abstract

Objective: This study aimed to evaluate the antidiabetic effects of the leaf extract of Canna indica L. in streptozotocin (STZ)-induced diabetic model. The present investigation indicated that the leaf of C. indica L. possessed significant antihyperglycemic potential which may prove the claimed use of the plant in amelioration of diabetes.


Methods: Male Swiss albino mice fasted for 12–14 h and weights were recorded prior to the induction of diabetes. Experimental diabetes was induced by a single intraperitoneal injection of 150 mg/kg of STZ, freshly dissolved in 0.1 M citrate buffer (pH=4.5). Then, the solution was immediately administered intraperitoneally to each mouse. Thirty minutes after the injection, the mice were allowed free access to food and water. After 6 h STZ injection, mice were given a 5% dextrose solution for the next 24 h. The development of diabetes was confirmed after 3 days of the STZ injection and mice with fasting blood glucose level (BGL) >200 mg/dl were considered as diabetic and were selected for the experiments. After successfully developing the diabetes animals were divided into six groups and each group contains six mice. Group I: Normal control mice administered vehicle only; Group II: Diabetic control mice administered vehicle only; Group III: Tested mice administered glibenclamide 5 mg/kg; and Group IV–VI: Tested mice administered C. indica L. at doses of 100, 200, and 400 mg/kg, respectively. All groups received treatments once daily for 14 days. The fasting BGL and body weight were determined at 0, 7th, and 14th days.


Results: The effect of C. indica L. on BGL having significant (p<0.05 and p<0.001) reduction in BGL starting 1 h when compared to the negative control. Administration of glucose (2 g/kg) to the mice produced significant (p<0.001) increase in BGL 30 min following 1 h after glucose loading, confirming the induction of hyperglycemia. The extract with three doses (100, 200, and 400 mg/kg) showed a significant reduction in BGL. The phytochemical screening of the leaves extract was done for the presence of alkaloid, saponin, terpene, carbohydrate, steroid, protein, cholesterol, flavonoids. Amino acid was absent in C. indica L. leaves.


Conclusion: It is concluded from this study that the alcohol extracts of C. indica L. leaves possess significant antihyperglycemic effects.

Keywords: Canna indica L., Physicochemical, Phytochemical, Antihyperglycemic

References

1. Dhodi JB, Mestry SN, Juvekar AR. Diabetic nephropathy: Genesis, prevention and treatment. Int J Pharm Pharm Sci 2014;6:42-7.
2. Kumar KE, Jyotsna PD, Ram KR, Swathi P, Gupta MN. Parmacodynamic and pharmacokinetic drug interaction of gliclazide and risperidone in animal models. Int J Pharm Pharm Sci 2012;4:659-62.
3. Kishore L, Kaur N, Singh R. Distinct biomarkers for early diagnosis of diabetic nephropathy. Curr Diabetes Rev 2017;13:598-605.
4. Kim Y, Park CW. New therapeutic agents in diabetic nephropathy. Korean J Int Med 2017;32:11-25.
5. Acharya VN. Diabetic and hypertensive nephropathy in India. J Assoc Physicians India 2011;59:143.
6. Pradeepa R, Mohan V. Prevalence of Type 2 diabetes and its complications in India and economic costs to the nation. Eur J Clin Nutr 2017;71:816-24.
7. Bener A, Al-Laftah F, Al-Hamaq AO, Daghash M, Abdullatef WK. A study of diabetes complications in an endogamous population: An emerging public health burden. Diabetes Metab Syndr 2014;8:108-14.
8. Tripathi SM, Singh DK. Molluscicidal activity of Punica granatum bark and Canna indica root. Braz J Med Biol Res 2000;33:1351-5.
9. Jamal P, Barkat AA, Amid A. Distribution of phenolics in various Malaysian medicinal plants. J Appl Sci 2010;10:2658-62.
10. Indrayan AK, Bhojak NK, Kumar N, Shatru A, Gaur A. Chemical composition and antimicrobial activity of the essential oil from the rhizome of Canna indica Linn. Indian J Chem B Org Med Chem 2011;50B:1136-9.
11. Joshi YM, Kadam VJ, Kaldhone PR. Antioxidant activity of methanolic extract of aerial parts of Canna indica L. J Pharm Res 2009;2:1712-5.
12. Nirmal SA, Shelke SM, Gagare PB, Jadhav PR, Dethe PM. Antinociceptive and anthelmintic activity of Canna indica. Nat Prod Res 2007;21:1042-7.
13. Prakash SL, Kumar AK. Antioxidant activity and wound healing potential of selected medicinal plants. J Chem Pharm Sci 2014;2:100-4.
14. Gupta A, Naraniwal M, Kothari V. Modern extraction methods for preparation of bioactive plant extracts. Int J Appl Nat Sci 2012;1:8-26.
15. OECD/OECDE, Test No. 407: Repeated Dose 28 day Oral Toxicity Study in Rodents; 2008.
16. Fröde TS, Medeiros YS. Animal models to test drugs with potential antidiabetic activity. J Ethnopharmacol 2008;115:173-83.
17. Mustaffa F, Indurkar J, Shojai MS. A review of Malaysian medicinal plants with potential antidiabetic activity. J Pharm Res 2011;4:4217-24.
18. Hammeso WW, Emiru YK, Getahun KA, Kahaliw W. Antidiabetic and antihyperlipidemic activities of the leaf latex extract of Aloe megalacantha baker (Aloaceae) in streptozotocin-induced diabetic model. Evid Based Complement Alternat Med 2019;2019:1-9.
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YADAV, P. K., and S. SS. “PRELIMINARY PHYTOCHEMICAL SCREENING, CHARACTERIZATION, AND ANTIDIABETIC ACTIVITY OF LEAF EXTRACT OF CANNA INDICA L. IN STREPTOZOTOCIN-INDUCED DIABETIC MODEL”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 2, Dec. 2019, pp. 58-62, doi:10.22159/ajpcr.2020.v13i2.36396.
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