COMPARATIVE STUDY BETWEEN OPTIMAL AND REDUCED FORMALIN-BASED FIXATIVE FOR NORMAL AND PATHOLOGICAL TISSUES

optimal fixative versus reduced formalin -based fixative

  • SAWSAN SALIH ALHAROON Department of Pathology and Forensic Medicine, College of Medicine, University of Basrah, Basrah, Iraq.
  • NAWAL MUSTAFA ABDULLAH Department of Anatomy, Histology and Embryology, Al-Zahra College of Medicine, University of Basrah, Basrah, Iraq.
  • NADA HASHIM MOHAMED Department of Anatomy, Al-Zahra College of Medicine, University of Basrah, Basrah, Iraq.

Abstract

Objectives: This study aims to use an alternative fixative compound with reduced concentration of formalin to minimize the exposure and related side effects.


Methods: Human normal and pathological tissue samples of different organs were immediately immersed in a modified fixative containing formalin, glycerin, ethanol, and hypotonic saline with pH around 7. Two time schedules were used at 8 and 12 h. Tissue samples were undergone routine processing procedure used in histopathological laboratories, scoring of samples was done, tissue sections of new compound fixative were examined for cytoplasmic, nuclear, and architectural properties and compared with 10% neutral buffered formalin (NBF).


Results: Tissue samples treated with modified fixative showed good and acceptable preservation of structural, cellular details with less harmful effects when compared with those preserved with routine 10% (NBF).


Conclusion: The suitability and effectiveness of reduced formalin-based fixative in histological and histopathological routine examination with minimal harmful effects for laboratory worker were clearly indicated in this study.

Keywords: Formalin, New fixative, Histopathology, Tissue sections

References

1. Titford ME, Horenstein MG. Histomorphologic assessment of formalin substitute fixatives for diagnostic surgical pathology. Arch Pathol Lab Med 2005;129:502-6.
2. Vincek V, Nassiri M, Nadji M, Morales AR. A tissue fixative that protects macromolecules (DNA, RNA, and protein) and histomorphology in clinical samples. Lab Invest 2003;83:1427-35.
3. Moelans CB, ter Hoeve N, van Ginkel JW, ten Kate FJ, van Diest PJ. Formaldehyde substitute fixatives. Analysis of macroscopy, morphologic analysis, and immunohistochemical analysis. Am J Clin Pathol 2011;136:548-56.
4. Zhang L, Tang X, Rothman N, Vermeulen R, Ji Z, Shen M, et al. Occupational exposure to formaldehyde, hematotoxicity, and leukemia-specific chromosome changes in cultured myeloid progenitor cells. Cancer Epidemiol Biomarkers Prev 2010;19:80-8.
5. Gardner MJ, Pannett B, Winter PD, Cruddas AM. A cohort study of workers exposed to formaldehyde in the British chemical industry: An update. Br J Ind Med 1993;50:827-34.
6. Akbar-Khanzadeh F, Mlynek JS. Changes in respiratory function after one and three hours of exposure to formaldehyde in non-smoking subjects. Occup Environ Med 1997;54:296-300.
7. Kulle TJ, Sauder LR, Hebel JR, Green DJ, Chatham MD. Formaldehyde dose-response in healthy nonsmokers. JAPCA 1987;37:919-924.
8. Lang I, Bruckner T, Triebig G. Formaldehyde and chemosensory irritation in humans: A controlled human exposure study. Regul Toxicol Pharmacol 2008;50:23-36.
9. Porter JA. Letter: Acute respiratory distress following formalin inhalation. Lancet 1975;2:603-4.
10. Rager JE, Smeester L, Jaspers I, Sexton KG, Fry RC. Epigenetic changes induced by air toxics: Formaldehyde exposure alters miRNA expression profiles in human lung cells. Environ Health Perspect 2011;119:494-500.
11. Kim KH, Jahan SA, Lee JT. Exposure to formaldehyde and its potential human health hazards. J Environ Sci Health C Environ Carcinog Ecotoxicol Rev 2011;29:277-99.
12. Costa S, Coelho P, Costa C, Silva S, Mayan O, Santos LS, et al. Genotoxic damage in pathology anatomy laboratory workers exposed to formaldehyde. Toxicology 2008;252:40-8.
13. Binetti R, Costamagna FM, Marcello I. Development of carcinogenicity classifications and evaluations: The case of formaldehyde. Ann Ist Super Sanita 2006;42:132-43.
14. International Agency for Research on Cancer. Overall Evaluations of Carcinogenicity. IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals to Humans. Lyon, France: International Agency for Research on Cancer; 1987. p. 440.
15. Schwilk E, Zhang L, Smith MT, Smith AH, Steinmaus C. Formaldehyde and leukemia: An updated meta-analysis and evaluation of bias. J Occup Environ Med 2010;52:878-86.
16. Hauptmann M, Stewart PA, Lubin JH, Freeman LE, Hornung RW, Herrick RF, et al. Mortality from lymphohematopoietic malignancies and brain cancer among embalmers exposed to formaldehyde. J Natl Cancer Inst 2009;101:1696-708.
17. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans Volume 100F: A Review of Human Carcinogens: Chemical Agents and Related Occupations. In Formaldehyde; 2012. Available from: http:// www.monographs.iarc.fr/ENG/Monographs/vol100F/mono100F-29.pdf.
18. Code of Federal Regulations. Occupational safety and health administration code of federal regulations: Toxic and hazardous substances. In: Formaldehyde. USA: Code of Federal Regulations; 2007. p. 1910-1048.
19. International Agency for Research on Cancer. Formaldehyde, 2-butoxyethanol, and 1-tertbutoxy-2propanol: IARC Monographs on the Evaluation of Carcinogenic Risks to Human. Geneva, Switzerland: International Agency for Research on Cancer; 2006. p. 88.
20. Cogliano VJ, Grosse Y, Baan RA, Straif K, Secretan MB, El Ghissassi F. Meeting report: Summary of IARC monographs on formaldehyde, 2-butoxyethanol, and 1-tert-butoxy-2-propanol. Environ Health Perspect 2005;113:1205-8.
21. Acton A, Harvey T, Grow MW. An examination of non-formalin-based fixation methods for Xenopus embryos. Dev Dyn 2005;233:1464-9.
22. Buesa RJ. Histology without formalin? Ann Diagn Pathol 2008;12:387 96.
23. Balbi T, Cicognani A, Esposti PD, Pierini G. Microwave processing and ethanol-based fixation in forensic pathology: An addendum of further scanning electron microscope observations. Am J Forensic Med Pathol 2009;30:242-5.
24. Warmington AR, Wilkinson JM, Riley CB. Evaluation of ethanol-based fixatives as a substitute for formalin in diagnostic clinical laboratories. J Histotechnol 2000;23:299-308.
25. Zanini C, Gerbaudo E, Ercole E, Vendramin A, Forni M. Evaluation of two commercial and three home-made fixatives for the substitution of formalin: A formaldehyde-free laboratory is possible. Environ Health 2012;11:59.
26. Masir N, Ghoddoosi M, Mansor S, Abdul-Rahman F, Florence CS, Mohamed-Ismail NA, et al. RCL2, a potential formalin substitute for tissue fixation in routine pathological specimens. Histopathology 2012;60:804-15.
27. Griesemer RA. Report of the federal panel on formaldehyde. Environ Health Perspect 1982;43:139-68.
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SALIH ALHAROON, S., N. MUSTAFA ABDULLAH, and N. H. MOHAMED. “COMPARATIVE STUDY BETWEEN OPTIMAL AND REDUCED FORMALIN-BASED FIXATIVE FOR NORMAL AND PATHOLOGICAL TISSUES”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 2, Dec. 2019, pp. 119-22, doi:10.22159/ajpcr.2020.v13i2.36400.
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