IN VITRO CYTOTOXIC ACTIVITY OF SECOISOLARICIRESINOL DIGLUCOSIDE ON HT-29, PA-1 CELL LINES, AND α-AMYLASE INHIBITORY ACTIVITY

  • GUNABHUSHANA DADDALA Department of Biotechnology, Jawaharlal Nehru Technological University, Ananthapur, Andhra Pradesh, India.
  • SWAROOPARANI A Department of Biotechnology, Jawaharlal Nehru Technological University, Ananthapur, Andhra Pradesh, India.

Abstract

Objective: The present study was conducted to evaluate the in vitro cytotoxic activity and α- amylase inhibitory activity of secoisolariciresinol diglucoside (SDG).


Methods: The cytotoxic activity was conducted on HT-29 (human colon cancer cell line) and PA-1 (human ovarian cancer cell line) by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay and the α- amylase inhibitory activity using acarbose as a standard. Both the tests were evaluated at different concentrations, 3.125–100 μg/ml and 50–2000 μg correspondingly and the concentration required for a 50% inhibition of viability (IC50) was determined graphically. The effect of the samples on the proliferation of HT-29 and PA-1 was expressed as the percentage cell viability.


Results: SDG exhibited a considerable dose- and time-dependent inhibition on both HT-29 and PA-1 and also observed a concentration-dependent α-amylase inhibitory activity that leads in reduction of starch hydrolysis and hence eventually to lowered glucose levels.


Conclusion: The present in vitro study concluded that SDG can be a potent anticancer and moderate hyperglycemic component.

Keywords: Secoisolariciresinol diglucoside, HT-29 cell lines, PA-1 cell lines, α-amylase inhibition

Author Biography

GUNABHUSHANA DADDALA, Department of Biotechnology, Jawaharlal Nehru Technological University, Ananthapur, Andhra Pradesh, India.

Biotechnology

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DADDALA, G., and S. A. “IN VITRO CYTOTOXIC ACTIVITY OF SECOISOLARICIRESINOL DIGLUCOSIDE ON HT-29, PA-1 CELL LINES, AND α-AMYLASE INHIBITORY ACTIVITY”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 2, Dec. 2019, pp. 168-73, doi:10.22159/ajpcr.2020.v13i2.36414.
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