• MUJAWAR SALMA BEGUM HUSSAIN Department of Biotechnology and Microbiology, Karnatak University, Dharwad, Karnataka, India.
  • MURIGENDRA B HIREMATH Department of Biotechnology and Microbiology, Karnatak University, Dharwad, Karnataka, India.


Objective: The objective of the study was to evaluate the effect of leaf extracts on foot ulcer and urinary tract pathogens from patients having diabetes.

Methods: The pus and urine sample from the patients having diabetes were collected and cultured on nutrient agar plates. The morphological characters were studied for the colonies obtained. Antimicrobial activity of ethanol and aqueous extracts was studied for Aegle marmelos leaves.

Results: Ethanol extract exhibited good results for both foot ulcer and urinary tract infected pathogens as compared to aqueous extract. Thin-layer chromatography was performed for methanol extract and then was subjected to column chromatography. The fractions obtained were again checked for the antibacterial activity and of three fractions, fraction 1 showed better results for pus and urine pathogens. Fourier-transformed infrared analysis was carried out for fraction 1 of methanol extract for verifying the presence of bioactive compounds.

Conclusion: From the present study, it can be terminated that the bioactive compounds derived from plant extracts can be used for the development of new pharmaceutical drugs to reach out to various therapeutic needs.

Keywords: Medicinal plant, Antimicrobial activity, Aegle marmelos, Thin-layer chromatography, Column chromatography, Fourier-transformed infrared


1. Grover JK, Yadav S, Vats V. Medicinal plants of India with anti-diabetic potential. J Ethnopharmacol 2002;81:81-100.
2. Priyanka, Rita S. A systematic review on Indian floral biodiversity as eminent reserves for alternative treatment strategy of diabetes mellitus. Int J Pharm Pharm Sci 2016;8:10-9.
3. Eloff JN. Which extractant should be used for the screening and isolation of antimicrobial components from plants? J Ethnopharmacol 1998;60:1-8.
4. Bowler PG, Davies BJ. The microbiology of infected and noninfected leg ulcers. Int J Dermatol 1999;38:573-8.
5. Stapleton A. Urinary tract infections in patients with diabetes. Am J Med 2002;113:80-4.
6. Foxman B, Somsel P, Tallman P, Gillespie B, Raz R, Colodner R, et al. Urinary tract infection among women aged 40 to 65: Behavioral and sexual risk factors. J Clin Epidemiol 2001;54:710-8.
7. Badam L, Bedekar SS, Sonawane KB, Joshi SP. In vitro antiviral activity of bael (Aegle marmelos Corr) upon human coxsackieviruses B1-B6. J Commun Dis 2002;34:88-99.
8. Porter IA, Brodie J. Boric acid preservation of urine samples. Br Med J 1969;2:353-5.
9. Breed RS, Murray EG, Smith NR. Bergey’s Manual of Determinative Bacteriology. Baltimore: The Williams and Wilkins Company; 1962.
10. Dubey RC. The Practical book of microbiology. 2nd ed. New York: Times Books; 2007. p. 37-9.
11. Jerine PS, Evan PS. Global current trends in natural products for diabetes management: A review. Int J Pharm Pharm Sci 2016;8:20-8.
12. Anita P, Anthoni AS, Raj JS. In vitro antibacterial activity of Aegiceras corniculatum and Bruguiera cylindrica against isolated bacterial urinary tract infections. Int J Pharm Res Dev 2012;3:1120-5.
13. Rojas R, Bustamante B, Bauer J, Fernandez I, Albán J, Lock O. Antimicrobial activity of selected Peruvian medicinal plants. J Ethnopharmacol 2003;88:199-204.
14. Duraipandiyan V, Ayyanar M, Ignacimuthu S. Antimicrobial activity of some ethnomedicinal plants used by paliyar tribe from Tamil Nadu, India. BMC Complement Altern Med 2006;6:35.
15. Parekh J, Chanda S. In vitro antimicrobial activity and phytochemical analysis of some Indian medicinal plants. Turk J Biol 2007;31:53-8.
16. Lakshmi SS, Chelladurai G, Suresh B. In vitro studies on medicinal plants used against bacterial diabetic foot ulcer (BDFU) and urinary tract infected (UTI) causing pathogens. J Parasit Dis 2014;40:667-73.
17. Venkatesan D, Karunakaran M, Kumar S, Palaniswamy P, Ramesh G. Antimicrobial activity of Aegle marmelos against pathogenic organism compared with control drug. J Ethnobot Leaflets 2009;13:968-74.
18. Francisco A, Harborne JB, editors. The Flavonoids Advances in Research Since 1986. Vol. 6. London, U.K: Chapman and Hall; 1995. p. 55.
19. Rice-Evans CA, Miller NJ, Bolwell PG, Bramley PM, Pridham JB. The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free Radic Res 1995;22:375-83.
20. Krupa AN, Raghavan V. Biosynthesis of silver nanoparticles using Aegle marmelos (Bael) fruit extract and its application to prevent adhesion of Bacteria: A strategy to control microfouling. Bioinorg Chem Appl 2014;2014:1-8.
139 Views | 107 Downloads
How to Cite
MUJAWAR SALMA BEGUM HUSSAIN, and MURIGENDRA B HIREMATH. “IN VITRO EVALUATION OF AEGLE MARMELOS LEAF EXTRACTS ON FOOT ULCER AND URINARY TRACT INFECTED PATHOGENS FROM DIABETIC PATIENTS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 1, Dec. 2019, pp. 229-33, doi:10.22159/ajpcr.2020.v13i1.36514.
Original Article(s)